FlyBase Allele Report: Dmel\PlexA[GD14483]

General Information

Symbol

Dmel\PlexA^GD14483

Species

D. melanogaster

Name

FlyBase ID

FBal0210356

Feature type

allele

Associated gene

Dmel\PlexA

Associated Insertion(s)

Carried in Construct

P{GD14483}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD14483}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD14483

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^VGlut-OK371

(Syed et al., 2016)

abnormal neuroanatomy | adult stage, with Scer\GAL4^GMR60B11

(Syed et al., 2016)

abnormal neuroanatomy | adult stage, with Scer\GAL4^{VGlut.VGN6341}

(Syed et al., 2016)

abnormal neuroanatomy | adult stage, with Scer\GAL4^VGlut-OK371

(Syed et al., 2016)

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

abnormal size | oogenesis, with Scer\GAL4^NP1624

(Stedden et al., 2019)

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

partially lethal, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

some die during pupal stage, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible | oogenesis, with Scer\GAL4^NP1624

(Stedden et al., 2019)

Phenotype Manifest In

axon | adult stage, with Scer\GAL4^GMR60B11

(Syed et al., 2016)

axon | adult stage, with Scer\GAL4^{VGlut.VGN6341}

(Syed et al., 2016)

axon | adult stage, with Scer\GAL4^VGlut-OK371

(Syed et al., 2016)

egg, with Scer\GAL4^NP1624

(Stedden et al., 2019)

motor neuron | adult stage, with Scer\GAL4^GMR60B11

(Syed et al., 2016)

motor neuron | adult stage, with Scer\GAL4^{VGlut.VGN6341}

(Syed et al., 2016)

motor neuron | adult stage, with Scer\GAL4^VGlut-OK371

(Syed et al., 2016)

somatic muscle cell of leg, with Scer\GAL4^GMR60B11

(Syed et al., 2016)

somatic muscle cell of leg, with Scer\GAL4^{VGlut.VGN6341}

(Syed et al., 2016)

somatic muscle cell of leg, with Scer\GAL4^VGlut-OK371

(Syed et al., 2016)

Detailed Description

Statement

Reference

Eggs from females expressing PlexA^GD14483 under the control of Scer\GAL4^NP1624 are rounder than from controls.

Follicle cell clones expressing PlexA^GD14483 under the control of Scer\GAL4^Act5C.PU show mostly normal basal protrusions.

(Stedden et al., 2019)

Expression of PlexA^GD14483 under the control of Scer\GAL4^VGlut-OK371 results in defective walking pattern (uncoordinated walk with short steps and leg dragging) and impaired innervation of the leg muscles in proximal femur (failed defasciculation or insufficient elongation of axon branches) as well as distal tibia (increase in the number of branches that exit the main nerve). Similarly, expression under the Scer\GAL4^{VGlut.VGN6341} driver results in axon mis-projection phenotypes in the leg muscles - lack of innervation of the muscles of the femur and excessive innervation of the muscles of the tibia, expression under Scer\GAL4^GMR60B11 also leads to lack of innervation in the femur.

(Syed et al., 2016)

Adults expressing PlexA^GD14483 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

(Neely et al., 2010)

Adults expressing PlexA^GD14483 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development