FlyBase Allele Report: Dmel\ds[ecto.UAS]

General Information

Symbol

Dmel\ds^ecto.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0213079

Feature type

allele

Associated gene

Dmel\ds

Associated Insertion(s)

Carried in Construct

P{UAS-ds.ecto}

Also Known As

UAS.ectoDs

Key Links

Transgenic product class

inframe_deletion

(Casal et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Casal et al., 2006)

Mutagen

in vitro construct

(Casal et al., 2006)

Progenitor genotype

ds^UAS.cTa

(Casal et al., 2006)

Carried in construct

P{UAS-ds.ecto}

Cytology

Description

Deletion of the ds cytosolic domain.

(Casal et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity | cell non-autonomous | somatic clone, with Scer\GAL4^αTub84B.PL

(Casal et al., 2006)

abnormal planar polarity | heat sensitive, with Scer\GAL4^ptc-559.1, Scer\GAL80^ts.αTub84B

(Repiso et al., 2010)

Phenotype Manifest In

trichome & abdominal tergite | somatic clone | cell non-autonomous, with Scer\GAL4^αTub84B.PL

(Casal et al., 2006)

ventral denticle belt | heat sensitive, with Scer\GAL4^ptc-559.1, Scer\GAL80^ts.αTub84B

(Repiso et al., 2010)

Detailed Description

Statement

Reference

Expression of ds^{ecto.Scer\UAS} under the control of Scer\GAL4^ptc-559.1 and Scer\GAL80^ts.αTub84B at 17[o]C has no effect on denticle belt polarity. However, growth at 29[o]C results in severe denticle polarity defects. Defects are also seen when the temperature is shifted from 17[o]C to 29[o]C before the moult to second stage larva. The converse shift, from 29[o]C to 17[o]C at the second instar larval stage results in wild-type denticle belt polarity.

(Repiso et al., 2010)

Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) that are expressing ds^{ecto.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL in a wild-type background usually result in a reversal of cell polarity in wild-type cells posterior to the clone (hairs behind the clone point forwards), with a range of several cells.

Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) that are expressing ds^{ecto.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL in a ds^unspecified background do not change the cell polarity of cells surrounding the clone.

Clones in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are mutant for ds^unspecified and are also expressing ds^{ecto.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL can reverse the cell polarity of cells posterior to the clone.

(Casal et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^αTub84B.PL, ds^UAO71, ds^ecto.UAS, ft¹⁵ has abnormal planar polarity | somatic clone | cell non-autonomous phenotype, suppressible | somatic clone by fj^UAS.cZa/Scer\GAL4^αTub84B.PL

(Brittle et al., 2010)

Other

Statement

Reference

Scer\GAL4^αTub84B.PL, ds^UAO71, ds^ecto.UAS, ft¹⁵ has abnormal planar polarity | somatic clone | cell non-autonomous phenotype

(Brittle et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^αTub84B.PL, ds^UAO71, ds^ecto.UAS, ft¹⁵ has trichome & tergite | cell non-autonomous | somatic clone phenotype, suppressible | somatic clone by fj^UAS.cZa/Scer\GAL4^αTub84B.PL

(Brittle et al., 2010)

Other

Statement

Reference

Scer\GAL4^αTub84B.PL, ds^UAO71, ds^ecto.UAS, ft¹⁵ has trichome & tergite | somatic clone | cell non-autonomous phenotype

(Brittle et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

ds^UAO71 ft¹⁵ double mutant clones in the dorsal adult abdomen where the clones are also expressing ds^{ecto.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL strongly repolarise hairs behind the clone, reversing their polarity (100% of clones repolarise with a maximum range of up to seven cells). This repolarisation is largely suppressed by co-expression of fj^Scer\UAS.cZa within the clone (48% of clones show some repolarisation with a range of one to three cells).

(Brittle et al., 2010)

The reversal of cell polarity that is seen in cells posterior to clones in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are expressing ds^{ecto.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL is still seen if the clones are induced in a stan^- background (the cells within the clone are stan^E59/stan^E59 while the cells surrounding the clone are stan³/stan^E59).

The reversal of cell polarity that is seen in cells posterior to clones in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are expressing ds^{ecto.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL is still seen if the clones are induced in a fz^unspecified stan^- background (cells within the clone are stan^E59/stan^E59 while the cells surrounding the clone are stan³/stan^E59).

Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) that are expressing ds^{ecto.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL in a ft¹²/ft^unspecified background do not change the cell polarity of cells surrounding the clone.

Clones in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are mutant for ft^unspecified and are also expressing ds^{ecto.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL can reverse the cell polarity of cells posterior to the clone.

Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are co-expressing both fj^Scer\UAS.cZa and ds^{ecto.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL result in a reversal of cell polarity in cells anterior to the clone.

Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are co-expressing both ft^Scer\UAS.cMa and ds^{ecto.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL result in a reversal of cell polarity in cells anterior to the clone.

The reversal of cell polarity that is seen in cells posterior to clones in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are expressing ds^{ecto.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL is enhanced if the clones are induced in a fj^unspecified background compared to a wild-type background; the repolarisation occurs over a greater range in the fj^unspecified background.

(Casal et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ds^{ecto.Scer\UAS}

(Brittle et al., 2010)

ds^ecto.UAS

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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