FlyBase Allele Report: Dmel\Lkb1[X5]

General Information

Symbol

Dmel\Lkb1^X5

Species

D. melanogaster

Name

FlyBase ID

FBal0213122

Feature type

allele

Associated gene

Dmel\Lkb1

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Lee et al., 2006)

Mutagen

P-element activity

(Lee et al., 2006)

Progenitor genotype

Cytology

Description

Imprecise excision of lkb1^EP-1 results in a specific deletion of the lkb1 locus (76208-77847th nucleotides of AE003701).

(Lee et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:13,676,752..13,678,391 [+]

Comment:

Imprecise excision deletion of nucleotides 76208-77847 (GB:AE003701).

(Lee et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity

(Lee et al., 2007)

abnormal mitotic cell cycle

(Lee et al., 2007)

decreased cell death

(Lee et al., 2006)

lethal - all die before end of pupal stage

(Lee et al., 2006)

some die during pupal stage

(Lee et al., 2006)

Phenotype Manifest In

embryonic/larval brain

(Lee et al., 2006)

follicle cell | somatic clone

(O'Farrell et al., 2017)

larval ventral nerve cord

(Lee et al., 2006)

Detailed Description

Statement

Reference

Lkb1^X5 somatic clones in the follicular epithelium exhibit multilayering in about a third of cases, which is not observed in controls.

(O'Farrell et al., 2017)

lkb1^X5 mutant eye disc clones do not show an increase in apoptosis following fasting compared to wild type controls.

(Zhang et al., 2014)

Mutant embryos derived from homozygous female germ-line clones contain cells with an excessive DNA content in the nucleus and have epithelial polarity defects.

Mutant larvae have mitotic and epithelial polarity defects.

(Lee et al., 2007)

lkb1^X5 mutants die before the mid-pupal stage.

During nerve cord condensation (embryonic stage 16), significantly fewer apoptotic cells are observed, both in the ventral nerve cord and embryonic head of lkb1^X5 mutants.

Homozygous lkb1^X5 mutant larvae exhibit dramatically enlarged brain hemispheres and ventral ganglion, compared to wild-type.

A small portion (~10%) of lkb1^X5 homozygotes exhibit various dorsal-open phenotypes in hatched or unhatched first-instar larvae.

(Lee et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Lkb1^X5 has abnormal mitotic cell cycle phenotype, suppressible by sqh^E20.E21

(Lee et al., 2007)

Lkb1^X5 has abnormal mitotic cell cycle phenotype, suppressible by AMPKα^TD.UAS/Scer\GAL4^hs.PB

(Lee et al., 2007)

Lkb1^X5 has abnormal cell polarity phenotype, suppressible by AMPKα^TD.UAS/Scer\GAL4^hs.PB

(Lee et al., 2007)

Lkb1^X5 has abnormal cell polarity phenotype, suppressible by sqh^E20.E21

(Lee et al., 2007)

Lkb1^X5 has decreased cell death phenotype, suppressible by hep^Act.UAS/Scer\GAL4^hs.PB

(Lee et al., 2006)

Enhancer of

Statement

Reference

Lkb1^X5 is an enhancer of abnormal starvation stress response | somatic clone phenotype of Axn¹²⁷

(Zhang et al., 2014)

Lkb1^X5 is an enhancer of increased cell death | somatic clone | nutrition conditional phenotype of Axn¹²⁷

(Zhang et al., 2014)

Lkb1^X5 is an enhancer of increased cell death | somatic clone phenotype of Axn^S044230, Rbf^15aΔ

(Zhang et al., 2014)

Suppressor of

Statement

Reference

Lkb1^X5 is a suppressor of abnormal cell polarity | somatic clone phenotype of Vps15⁵

(O'Farrell et al., 2017)

Lkb1^X5 is a suppressor | somatic clone of neoplasia | somatic clone | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, Vps15⁵

(O'Farrell et al., 2017)

Lkb1^X5 is a suppressor | somatic clone of abnormal developmental rate phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, Vps15⁵

(O'Farrell et al., 2017)

Lkb1^X5 is a suppressor of neoplasia | somatic clone | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI

(O'Farrell et al., 2017)

Lkb1^X5, Scer\GAL4^e22c is a suppressor of increased cell death phenotype of Scer\GAL4^e22c, Sln^{6.UAS.Tag:MYC}

(Jang et al., 2008)

NOT Suppressor of

Statement

Reference

lkb1[+]/Lkb1^X5 is a non-suppressor of increased cell death phenotype of Scer\GAL4^GMR.PF, egr^UAS.cLa

(Lee et al., 2006)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Lkb1^X5 has follicle cell | somatic clone phenotype, non-enhanceable by Vps15⁵

(O'Farrell et al., 2017)

Suppressed by

Statement

Reference

Lkb1^X5 has embryonic/larval brain phenotype, suppressible by hep^Act.UAS/Scer\GAL4^hs.PB

(Lee et al., 2006)

Lkb1^X5 has larval ventral nerve cord phenotype, suppressible by hep^Act.UAS/Scer\GAL4^hs.PB

(Lee et al., 2006)

Enhancer of

Statement

Reference

Lkb1^X5 is an enhancer of eye disc | somatic clone | nutrition conditional phenotype of Axn¹²⁷

(Zhang et al., 2014)

Lkb1^X5 is an enhancer of eye disc | somatic clone phenotype of Axn^S044230, Rbf^15aΔ

(Zhang et al., 2014)

Suppressor of

Statement

Reference

Lkb1^X5 is a suppressor of follicle cell | somatic clone phenotype of Vps15⁵

(O'Farrell et al., 2017)

Lkb1^X5 is a suppressor | somatic clone of eye-antennal disc | somatic clone | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, Vps15⁵

(O'Farrell et al., 2017)

Lkb1^X5 is a suppressor of eye-antennal disc | somatic clone | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI

(O'Farrell et al., 2017)

Lkb1^X5, Scer\GAL4^e22c is a suppressor of wing disc phenotype of Scer\GAL4^e22c, Sln^{6.UAS.Tag:MYC}

(Jang et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Vps15⁵, Lkb1^X5 double mutant somatic clones in the follicular epithelium exhibit multilayering at a frequency similar to that of Lkb1^X5 single clones rather than the high frequency observed in Vps15⁵ single clones. The mis-localization of cell polarity markers presented by Vps15⁵ homozygous follicle cell clones is also suppressed by Lkb1^X5 homozygosity in the clones.

The moderate overgrowth of eye-antennal discs bearing clones expressing Ras85D^V12.Scer\UAS under the control of Scer\GAL4^Act5C.PI is suppressed by Lkb1^X5 homozygosity in the clones.

The severe eye-antennal disc tumor phenotype and delayed pupation resulting from larval eye-antennal disc clones both homozygous for Vps15⁵ and expressing Ras85D^V12.Scer\UAS under the control of Scer\GAL4^Act5C.PI are both suppressed by additional Lkb1^X5 homozygosity in the clones.

(O'Farrell et al., 2017)

Scer\GAL4^e22c Sln^{6.Scer\UAS.T:Hsap\MYC}-mediated apoptosis upon butyrate treatment is considerably suppressed in a lkb1^X5 mutant background.

(Jang et al., 2008)

The epithelial polarity defects and genomic instability seen in lkb1^X5 larvae are suppressed by expression of SNF1A^TD.Scer\UAS under the control of Scer\GAL4^hs.PB.

The epithelial polarity defects and genomic polyploidy defects seen in lkb1^X5 larvae are suppressed by sqh^E20.E21.

(Lee et al., 2007)

Ectopic activation of JNK signaling by overexpression of hep^Act.Scer\UAS (under the control of Scer\GAL4^hs.PB) completely rescues the 'big brain' phenotype of lkb1^X5 mutants and restores embryonic apoptosis.

Trans-heterozygotic combinations between lkb1^X5 and bsk¹ or bsk² induces significant dorsal open phenotypes in unhatched first instar larvae.

Dosage reduction of lkb1^X5 does not affect Scer\GAL4^GMR.PF; egr^Scer\UAS.cla induced apoptosis.

(Lee et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Lkb1^X5 is rescued by Lkb1^UAS.cLa/Scer\GAL4^hs.PB

(Lee et al., 2006)

Comments

Transgenic expression of lkb1^Scer\UAS.cLa using a Scer\GAL4^hs.PB driver successfully rescues the lethality of lkb1^X5 mutants.

Ectopic expression of lkb1^Scer\UAS.cLa under the control of Scer\GAL4^hs.PB successfully suppresses the 'big brain' phenotype observed in lkb1^X5 mutants.

(Lee et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

LKB1^X5

(Choi et al., 2015, Jang et al., 2008, Lee et al., 2006)

Lkb1^X5

(Rackley et al., 2021, Couderc et al., 2017)

lkb1^X5

(O'Farrell et al., 2017, Zhang et al., 2014, Lee et al., 2012, Lee et al., 2007)

lkb1^x5

(Dogliotti et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (10)

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