FlyBase Allele Report: Dmel\AMPKα[TD.UAS]

General Information

Symbol

Dmel\AMPKα^TD.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0213121

Feature type

allele

Associated gene

Dmel\AMPKα

Associated Insertion(s)

Carried in Construct

P{UAS-AMPKα.TD}

Also Known As

UAS-AMPK^TD, UAS-dAMPK-TD

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Lee et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-AMPKα.TD}

Cytology

Description

UASt regulatory sequences drive expression of a constitutively active form of AMPKα.

(Lee et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

AMPKα

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by Hsap\LRRK2^{G2019S.UAS.Tag:MYC}

(Ng et al., 2017, Ng et al., 2012)

modeled by park¹

(Ng et al., 2012)

DOES NOT ameliorate Parkinson's disease

modeled by srl^KK100201

(Ng et al., 2017)

ameliorates Parkinson's disease 6

modeled by Pink1^B9

(Ham et al., 2021)

ameliorates Parkinson's disease 2

modeled by park¹

(Ham et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Scer\GAL4^elav.PLu-mediated expression of SNF1A^TD.Scer\UAS has no significant effect on phototaxis, neuromuscular junction size or excitatory junctional potentials.

(Dimitroff et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

AMPKα^TD.UAS, Scer\GAL4^elav.PLu is an enhancer of abnormal neurophysiology phenotype of Rheb^UAS.cPa, Scer\GAL4^elav.PLu

(Dimitroff et al., 2012)

Suppressor of

Statement

Reference

Scer\GAL4^ple.PF/AMPKα^TD.UAS is a suppressor of abnormal locomotor behavior | adult stage phenotype of Pink1^B9

(Ham et al., 2021)

Scer\GAL4^ple.PF/AMPKα^TD.UAS is a suppressor of decreased cell number | adult stage phenotype of Pink1^B9

(Ham et al., 2021)

Scer\GAL4^ple.PF/AMPKα^TD.UAS is a suppressor of abnormal locomotor behavior | adult stage phenotype of park¹

(Ham et al., 2021)

Scer\GAL4^ple.PF/AMPKα^TD.UAS is a suppressor of decreased cell number | adult stage phenotype of park¹

(Ham et al., 2021)

AMPKα^TD.UAS, Scer\GAL4^Ddc.PL is a suppressor of abnormal locomotor behavior | adult stage phenotype of Hsap\LRRK2^{G2019S.UAS.Tag:MYC}, Scer\GAL4^Ddc.PL

(Ng et al., 2017)

AMPKα^TD.UAS, Scer\GAL4^Ddc.PL is a suppressor of decreased cell number | adult stage phenotype of Hsap\LRRK2^{G2019S.UAS.Tag:MYC}, Scer\GAL4^Ddc.PL

(Ng et al., 2017)

AMPKα^TD.UAS, Scer\GAL4^elav.PLu is a suppressor of abnormal neuroanatomy phenotype of Rheb^UAS.cPa, Scer\GAL4^elav.PLu

(Dimitroff et al., 2012)

AMPKα^TD.UAS, Scer\GAL4^elav.PLu is a suppressor of abnormal phototaxis phenotype of Rheb^UAS.cPa, Scer\GAL4^elav.PLu

(Dimitroff et al., 2012)

AMPKα^TD.UAS/Scer\GAL4^hs.PB is a suppressor of abnormal cell polarity phenotype of Lkb1^X5

(Lee et al., 2007)

AMPKα^TD.UAS/Scer\GAL4^hs.PB is a suppressor of abnormal mitotic cell cycle phenotype of Lkb1^X5

(Lee et al., 2007)

NOT Suppressor of

Statement

Reference

AMPKα^TD.UAS, Fundc1^NIG.5676R, Scer\GAL4^hs.PB is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of park¹

(Ham et al., 2021)

AMPKα^TD.UAS, Fundc1^NIG.5676R, Scer\GAL4^hs.PB is a non-suppressor of decreased cell number | adult stage phenotype of park¹

(Ham et al., 2021)

AMPKα^TD.UAS, Fundc1^NIG.5676R, Scer\GAL4^hs.PB is a non-suppressor of increased cell death | adult stage phenotype of park¹

(Ham et al., 2021)

AMPKα^TD.UAS, Fundc1^NIG.5676R, Scer\GAL4^hs.PB is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of Pink1^B9

(Ham et al., 2021)

AMPKα^TD.UAS, Fundc1^NIG.5676R, Scer\GAL4^hs.PB is a non-suppressor of decreased cell number | adult stage phenotype of Pink1^B9

(Ham et al., 2021)

AMPKα^TD.UAS, Fundc1^NIG.5676R, Scer\GAL4^hs.PB is a non-suppressor of increased cell death | adult stage phenotype of Pink1^B9

(Ham et al., 2021)

AMPKα^TD.UAS, Scer\GAL4^Ddc.PL is a non-suppressor of decreased cell number | adult stage phenotype of Scer\GAL4^Ddc.PL, srl^KK100201

(Ng et al., 2017)

AMPKα^TD.UAS/srl^KK100201, Scer\GAL4^Ddc.PL is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of Hsap\LRRK2^{G2019S.UAS.Tag:MYC}, Scer\GAL4^Ddc.PL

(Ng et al., 2017)

AMPKα^TD.UAS/srl^KK100201, Scer\GAL4^Ddc.PL is a non-suppressor of decreased cell number | adult stage phenotype of Hsap\LRRK2^{G2019S.UAS.Tag:MYC}, Scer\GAL4^Ddc.PL

(Ng et al., 2017)

Other

Statement

Reference

AMPKα^TD.UAS, Scer\GAL4^Ddc.PL, srl^KK100201 has abnormal locomotor behavior | adult stage phenotype

(Ng et al., 2017)

Phenotype Manifest In

Enhancer of

Statement

Reference

AMPKα^TD.UAS, Scer\GAL4^elav.PLu is an enhancer of embryonic/larval neuromuscular junction phenotype of Rheb^UAS.cPa, Scer\GAL4^elav.PLu

(Dimitroff et al., 2012)

Suppressor of

Statement

Reference

Scer\GAL4^ple.PF/AMPKα^TD.UAS is a suppressor of dopaminergic PPM1 neuron | decreased number phenotype of Pink1^B9

(Ham et al., 2021)

Scer\GAL4^ple.PF/AMPKα^TD.UAS is a suppressor of dopaminergic PPM2 neuron | decreased number phenotype of Pink1^B9

(Ham et al., 2021)

Scer\GAL4^ple.PF/AMPKα^TD.UAS is a suppressor of dopaminergic PPM1 neuron | decreased number phenotype of park¹

(Ham et al., 2021)

Scer\GAL4^ple.PF/AMPKα^TD.UAS is a suppressor of dopaminergic PPM2 neuron | decreased number phenotype of park¹

(Ham et al., 2021)

AMPKα^TD.UAS, Scer\GAL4^Ddc.PL is a suppressor of dopaminergic PPL1 neuron | adult stage phenotype of Hsap\LRRK2^{G2019S.UAS.Tag:MYC}, Scer\GAL4^Ddc.PL

(Ng et al., 2017)

AMPKα^TD.UAS, Scer\GAL4^elav.PLu is a suppressor of photoreceptor cell R7 phenotype of Rheb^UAS.cPa, Scer\GAL4^elav.PLu

(Dimitroff et al., 2012)

AMPKα^TD.UAS, Scer\GAL4^elav.PLu is a suppressor of photoreceptor cell R8 phenotype of Rheb^UAS.cPa, Scer\GAL4^elav.PLu

(Dimitroff et al., 2012)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ple.PF/AMPKα^TD.UAS is a non-suppressor of dopaminergic PPL1 neuron | decreased number phenotype of Pink1^B9

(Ham et al., 2021)

AMPKα^TD.UAS, Fundc1^NIG.5676R, Scer\GAL4^hs.PB is a non-suppressor of dopaminergic PPM1 neuron | decreased number phenotype of Pink1^B9

(Ham et al., 2021)

AMPKα^TD.UAS, Fundc1^NIG.5676R, Scer\GAL4^hs.PB is a non-suppressor of dopaminergic PPM2 neuron | decreased number phenotype of Pink1^B9

(Ham et al., 2021)

AMPKα^TD.UAS, Fundc1^NIG.5676R, Scer\GAL4^hs.PB is a non-suppressor of mitochondrion | adult stage phenotype of Pink1^B9

(Ham et al., 2021)

AMPKα^TD.UAS, Fundc1^NIG.5676R, Scer\GAL4^hs.PB is a non-suppressor of flight muscle cell | adult stage phenotype of Pink1^B9

(Ham et al., 2021)

Scer\GAL4^ple.PF/AMPKα^TD.UAS is a non-suppressor of dopaminergic PPM1 neuron | decreased number phenotype of park¹

(Ham et al., 2021)

Scer\GAL4^ple.PF/AMPKα^TD.UAS is a non-suppressor of dopaminergic PPM2 neuron | decreased number phenotype of park¹

(Ham et al., 2021)

Scer\GAL4^ple.PF/AMPKα^TD.UAS is a non-suppressor of dopaminergic PPL1 neuron | decreased number phenotype of park¹

(Ham et al., 2021)

AMPKα^TD.UAS, Fundc1^NIG.5676R, Scer\GAL4^hs.PB is a non-suppressor of dopaminergic PPM1 neuron | decreased number phenotype of park¹

(Ham et al., 2021)

AMPKα^TD.UAS, Fundc1^NIG.5676R, Scer\GAL4^hs.PB is a non-suppressor of dopaminergic PPM2 neuron | decreased number phenotype of park¹

(Ham et al., 2021)

AMPKα^TD.UAS, Fundc1^NIG.5676R, Scer\GAL4^hs.PB is a non-suppressor of mitochondrion | adult stage phenotype of park¹

(Ham et al., 2021)

AMPKα^TD.UAS, Fundc1^NIG.5676R, Scer\GAL4^hs.PB is a non-suppressor of flight muscle cell | adult stage phenotype of park¹

(Ham et al., 2021)

Scer\GAL4^ple.PF/AMPKα^TD.UAS is a non-suppressor of dopaminergic PPM1 neuron | decreased number phenotype of Pink1^B9

(Ham et al., 2021)

Scer\GAL4^ple.PF/AMPKα^TD.UAS is a non-suppressor of dopaminergic PPM2 neuron | decreased number phenotype of Pink1^B9

(Ham et al., 2021)

AMPKα^TD.UAS, Scer\GAL4^Ddc.PL is a non-suppressor of dopaminergic PPL1 neuron | adult stage phenotype of Scer\GAL4^Ddc.PL, srl^KK100201

(Ng et al., 2017)

AMPKα^TD.UAS/srl^KK100201, Scer\GAL4^Ddc.PL is a non-suppressor of dopaminergic PPL1 neuron | adult stage phenotype of Hsap\LRRK2^{G2019S.UAS.Tag:MYC}, Scer\GAL4^Ddc.PL

(Ng et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of AMPKα^TD.Scer\UAS fails to suppress the dopaminergic neuron loss seen in flies expressing srl^KK100201 under the control of Scer\GAL4^Ddc.PL, and additionally leads to climbing defects in these flies, as compared to controls.

(Ng et al., 2017)

Co-expression of SNF1A^TD.Scer\UAS significantly rescues the phototaxis and axon guidance phenotypes, while it enhances the synapse overgrowth and excitatory junctional potential phenotypes, of Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu flies.

(Dimitroff et al., 2012)

Expression of SNF1A^TD.Scer\UAS under the control of Scer\GAL4^how-24B suppresses the mitochondrial abnormalities and climbing ability defects seen in park¹ mutant flies. The wing posture defects seen in park¹ mutant flies are also rescued.

(Ng et al., 2012)

Expression of SNF1A^TD.Scer\UAS under the control of Scer\GAL4^fat does not rescue lipid levels in Sik3⁴⁸ flies.

(Wang et al., 2011)

The epithelial polarity defects and genomic instability seen in lkb1^X5 larvae are suppressed by expression of SNF1A^TD.Scer\UAS under the control of Scer\GAL4^hs.PB.

(Lee et al., 2007)

Xenogenetic Interactions

Statement

Reference

Co-expression of AMPKα^TD.Scer\UAS fully suppresses the climbing defects and loss of dopaminergic neurons seen in flies expressing Hsap\LRRK2^{G2019S.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Ddc.PL.

Co-expression of both srl^KK100201 and AMPKα^TD.Scer\UAS fails to rescue the climbing defects and loss of dopaminergic neurons seen in flies expressing Hsap\LRRK2^{G2019S.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Ddc.PL.

(Ng et al., 2017)

Expression of SNF1A^TD.Scer\UAS suppresses the climbing defects seen in flies expressing Hsap\LRRK2^{G2019S.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Ddc.PL. The protocerebral posterior lateral 1 (PPL1) dopaminergic neuron loss is also suppressed. The low dopamine levels seen in flies expressing Hsap\LRRK2^{G2019S.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Ddc.PL are also restored in the presence of SNF1A^TD.Scer\UAS. Expression of SNF1A^TD.Scer\UAS also rescues the climbing defects and mitochondrial abnormalities seen when Hsap\LRRK2^{G2019S.Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^how-24B.

(Ng et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

AMPK^TD

(Lee et al., 2007)

AMPKα^TD.Scer\UAS

AMPKα^TD.UAS

SNF1A^TD.Scer\UAS

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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