FlyBase Allele Report: Dmel\Lkb1[UAS.cLa]

General Information

Symbol

Dmel\Lkb1^UAS.cLa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Lee

FlyBase ID

FBal0213123

Feature type

allele

Associated gene

Dmel\Lkb1

Associated Insertion(s)

Carried in Construct

P{UAS-Lkb1.L}

Also Known As

UAS-LKB1^WT

Key Links

Transgenic product class

cDNA

(Lee et al., 2006)

wild_type

(Lee et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Lee et al., 2006)

wild_type

(Lee et al., 2006)

Mutagen

in vitro construct

(Lee et al., 2007, Lee et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-Lkb1.L}

Cytology

Description

UASt regulatory sequences drive expression of wild-type Lkb1 coding sequences derived from the full-length cDNA GM01569.

(Lee et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Lkb1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death, with Scer\GAL4^GMR.PF

(Jang et al., 2008, Lee et al., 2006)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Jang et al., 2008, Lee et al., 2006)

wing, with Scer\GAL4^ap-md544

(Jang et al., 2008, Lee et al., 2006)

wing, with Scer\GAL4^Bx-MS1096

(Lee et al., 2006)

wing blade, with Scer\GAL4^Bx-MS1096

(Lee et al., 2006)

wing disc, with Scer\GAL4^Bx-MS1096

(Lee et al., 2006)

wing disc, with Scer\GAL4^FRT.Act5C

(Lee et al., 2006)

Detailed Description

Statement

Reference

Expression of lkb1^Scer\UAS.cLa in the dorsal wing (under the control of Scer\GAL4^ap-md544) results in 'bent-up' small wings.

Expression of lkb1^Scer\UAS.cLa in the eye (under the control of Scer\GAL4^GMR.PF) results in a small and rough eye phenotype.

Expression of lkb1^Scer\UAS.cLa in the developing eye, under the control of Scer\GAL4^GMR.PF does not affect neuronal differentiation.

Apoptosis induced by expression of lkb1^Scer\UAS.cLa under the control of Scer\GAL4^e22c, is further enhanced by butyrate treatment.

(Jang et al., 2008)

Eye-specific expression of lkb1^Scer\UAS.cLa (under the control of Scer\GAL4^GMR.PF) induces a slight reduction in overall eye size. This reduction becomes more severe with elevated lkb1 levels.

Dorsal tissue-specific expression of lkb1^Scer\UAS.cLa (under the control of Scer\GAL4^ap-md544) induces a dramatic 'bent-up' wing phenotype, in a dose-dependent manner.

Scer\GAL4^Bx-MS1096-driven expression of lkb1^Scer\UAS.cLa dose-dependently reduces the overall wing size.

lkb1^Scer\UAS.cLa-expressing cells (generated through clonal analysis in wing imaginal discs with Scer\GAL4^{Scer\FRT.Act5C}) expand very poorly. However, this does not cause any alterations in the bristle size.

lkb1^Scer\UAS.cLa-expressing cells (generated through clonal analysis in wing imaginal discs with Scer\GAL4^Bx-MS1096), while displaying a dramatic reduction in cell expansion, fail to reduce wing marginal bristle size in the adult.

Overexpression of lkb1^Scer\UAS.cLa under the control of Scer\GAL4^GMR.PF does not affect S-phase cell number with respect to the control tissues (as assayed with BrdU). Similarly, there is no change in the phospho-His3 staining pattern, indicating that the G2-M cel cycle transition is also unaffected. However, staining with acridine orange, which specifically labels those dying cells with disrupted membrane integrity, shows prominent death signals, compared to controls, specifically in the region of lkb1^Scer\UAS.cLa. Furthermore, TUNEL staining of the eye and wing discs also confirms the existence of extensive apoptosis in the lkb1-expressing cells.

(Lee et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Lkb1^UAS.cLa, Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by Sln^EP2245, Scer\GAL4^GMR.PF

(Jang et al., 2008)

Suppressed by

Statement

Reference

Lkb1^UAS.cLa, Scer\GAL4^GMR.PF, Sln^EP2245 has increased cell death phenotype, suppressible by Diap1^UAS.cHa, Scer\GAL4^GMR.PF

(Jang et al., 2008)

Lkb1^UAS.cLa, Scer\GAL4^GMR.PF, Sln^EP2245 has increased cell death phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PF

(Jang et al., 2008)

Lkb1^UAS.cLa, Scer\GAL4^GMR.PF has increased cell death phenotype, suppressible by Diap1^UAS.cHa, Scer\GAL4^GMR.PF

(Lee et al., 2006)

Lkb1^UAS.cLa, Scer\GAL4^GMR.PF has increased cell death phenotype, suppressible by hep[+]/hep^r75, Scer\GAL4^GMR.PF

(Lee et al., 2006)

NOT suppressed by

Statement

Reference

Lkb1^UAS.cLa, Scer\GAL4^GMR.PF has increased cell death phenotype, non-suppressible by p53^Ct.GUS, Scer\GAL4^GMR.PF

(Lee et al., 2006)

NOT Suppressor of

Statement

Reference

Lkb1^UAS.cLa/Scer\GAL4^hs.PB is a non-suppressor of abnormal mitotic cell cycle | larval stage phenotype of AMPKα^D1

(Lee et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

Lkb1^UAS.cLa, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Sln^EP2245, Scer\GAL4^GMR.PF

(Jang et al., 2008)

Lkb1^UAS.cLa, Scer\GAL4^ap-md544 has wing phenotype, enhanceable by Sln^EP2245, Scer\GAL4^ap-md544

(Jang et al., 2008)

Lkb1^UAS.cLa, Scer\GAL4^Bx-MS1096 has wing phenotype, enhanceable by Sln^EP2245, Scer\GAL4^Bx-MS1096

(Jang et al., 2008)

Lkb1^UAS.cLa, Scer\GAL4^Bx-MS1096 has wing phenotype, enhanceable by msn^UAS.cSa, Scer\GAL4^Bx-MS1096

(Lee et al., 2006)

Lkb1^UAS.cLa, Scer\GAL4^Bx-MS1096 has wing phenotype, enhanceable by puc^E69/puc[+], Scer\GAL4^Bx-MS1096

(Lee et al., 2006)

Lkb1^UAS.cLa, Scer\GAL4^Bx-MS1096 has wing blade phenotype, enhanceable by hep^Act.UAS, Scer\GAL4^Bx-MS1096

(Lee et al., 2006)

Lkb1^UAS.cLa, Scer\GAL4^Bx-MS1096 has wing phenotype, enhanceable by hep^Act.UAS, Scer\GAL4^Bx-MS1096

(Lee et al., 2006)

Lkb1^UAS.cLa, Scer\GAL4^Bx-MS1096 has wing blade phenotype, enhanceable by bsk^UAS.cBa, Scer\GAL4^Bx-MS1096

(Lee et al., 2006)

Lkb1^UAS.cLa, Scer\GAL4^Bx-MS1096 has wing phenotype, enhanceable by bsk^UAS.cBa, Scer\GAL4^Bx-MS1096

(Lee et al., 2006)

Lkb1^UAS.cLa, Scer\GAL4^Bx-MS1096 has wing blade phenotype, enhanceable by msn^UAS.cSa, Scer\GAL4^Bx-MS1096

(Lee et al., 2006)

Suppressed by

Statement

Reference

Lkb1^UAS.cLa, Scer\GAL4^GMR.PF, Sln^EP2245 has eye phenotype, suppressible by Sln^RNAi.Sym.UAS, Scer\GAL4^GMR.PF

(Jang et al., 2008)

Lkb1^UAS.cLa, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Diap1^UAS.cHa, Scer\GAL4^GMR.PF

(Jang et al., 2008)

Lkb1^UAS.cLa, Scer\GAL4^GMR.PF, Sln^EP2245 has eye phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PF

(Jang et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Down-regulation of Sln through expression of Sln^{dsRNA.Sym.Scer\UAS} in the eye dramatically suppresses the narrow eye phenotype induced by lkb1^Scer\UAS.cLa and Sln^EP2245 (all under the control of Scer\GAL4^GMR.PF).

Down-regulation of Sln using two copies of Sln^{dsRNA.Sym.Scer\UAS} restores the reduced wing size observed through expression of lkb1^Scer\UAS.cLa (under the control of Scer\GAL4^Bx-MS1096).

(Jang et al., 2008)

The fraction of SNF1A^D1 mitotic larval brain neuroblasts that show extreme polyploidy is not changed if the larvae are also co-expressing lkb1^Scer\UAS.cLa under the control of Scer\GAL4^hs.PB.

(Lee et al., 2007)

lkb1^Scer\UAS.cLa-induced apoptosis is not suppressed by expression of the dominant-negative p53^Ct.GUS, both under the control of Scer\GAL4^GMR.PF.

lkb1^Scer\UAS.cLa-induced apoptosis is completely suppressed by expression of th^Scer\UAS.cHa, both under the control of Scer\GAL4^GMR.PF. Interestingly, even when lkb1-induced apoptosis is completely blocked by th, the BrdU and His3 staining patterns do not deviate from the wild-type patterns.

A puc^E69/+ background dramatically enhances the lkb1^Scer\UAS.cLa-Scer\GAL4^Bx-MS1096 small wing phenotype.

Simultaneous expression of hep^Act.Scer\UAS with lkb1^Scer\UAS.cLa (both under the control of Scer\GAL4^Bx-MS1096 strongly enhances the lkb1^Scer\UAS.cLa phenotype, resulting in severe disruption in the structure and size of the wing blades.

Simultaneous expression of bsk^Scer\UAS.cBa with lkb1^Scer\UAS.cLa (both under the control of Scer\GAL4^Bx-MS1096 strongly enhances the lkb1^Scer\UAS.cLa phenotype, resulting in severe disruption in the structure and size of the wing blades.

Simultaneous expression of msn^Scer\UAS.cSa with lkb1^Scer\UAS.cLa (both under the control of Scer\GAL4^Bx-MS1096 strongly enhances the lkb1^Scer\UAS.cLa phenotype, resulting in severe disruption in the structure and size of the wing blades.

A hep^r75 mutant background suppresses JNK pathway activation normally found when lkb1^Scer\UAS.cLa is expressed by either Scer\GAL4^GMR.PF or Scer\GAL4^ap-md544. In addition, Scer\GAL4^GMR.PF>lkb1^Scer\UAS.cLa-induced apoptosis in eye imaginal discs is also completely suppressed.

Dosage reduction of grim, rpr, and W genes, through the deficiency Df(3L)H99 alleviates lkb1^Scer\UAS.cLa-Scer\GAL4^GMR.PF-induced apoptosis.

(Lee et al., 2006)

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cHa with both lkb1^Scer\UAS.cLa and Sln^EP2245 considerably suppresses the apoptotic cell death induced by lkb1^Scer\UAS.cLa and Sln^EP2245 co-expression in the eye (all under the control of Scer\GAL4^GMR.PF).

(Jang et al., 2008)

Complementation and Rescue Data

Partially rescued by

Lkb1^UAS.cLa is partially rescued by Scer\GAL4^ap-md544/Lkb1^K201I.UAS

(Lee et al., 2006)

Rescues

Lkb1^UAS.cLa/Scer\GAL4^hs.PB rescues Lkb1^X5

(Lee et al., 2006)

Comments

A partial downregulation of JNK activity by expression of kinase-dead lkb1^{K201I.Scer\UAS} modestly rescues the adult phenotype induced by Scer\GAL4^ap-md544-driven lkb1^Scer\UAS.cLa expression.

Transgenic expression of lkb1^Scer\UAS.cLa using a Scer\GAL4^hs.PB driver successfully rescues the lethality of lkb1^X5 mutants.

Ectopic expression of lkb1^Scer\UAS.cLa under the control of Scer\GAL4^hs.PB successfully suppresses the 'big brain' phenotype observed in lkb1^X5 mutants.

(Lee et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Lkb1^Scer\UAS.cLa

Lkb1^UAS.cLa

lkb1^Scer\UAS.cLa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Lee

Secondary FlyBase IDs

References (5)

Report Sections

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