FB2024_03 , released June 25, 2024
Allele: Dmel\Rab1S25N.UAS.YFP
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General Information
Symbol
Dmel\Rab1S25N.UAS.YFP
Species
D. melanogaster
Name
FlyBase ID
FBal0215381
Feature type
allele
Associated gene
Dmel\Rab1
Associated Insertion(s)
Carried in Construct
P{UAST-YFP.Rab1.S25N}
Also Known As
Rab1DN, UAS-YFP-Rab1S25N
Key Links
Transgenic product class
dominant_negative_variant
(Zhang et al., 2007)
missense_variant
(Zhang et al., 2007)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Zhang et al., 2007)
missense_variant
(Zhang et al., 2007)
Mutagen
in vitro construct
(Zhang et al., 2007, Zhang et al., 2007)
Progenitor genotype
Carried in construct
P{UAST-YFP.Rab1.S25N}
Cytology
Description

Amino acid replacement: S25N.

(Zhang et al., 2007)

UASt regulatory sequences drive expression of a predicted dominant negative form of Rab1 that is tagged at the N-terminal end with YFP.

(Zhang et al., 2007)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Rab1
Tagged with
YFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expressing Rab1S25N.UAS.YFP from the middle third instar larval stage onwards under the combined control of Scer\GAL4GSG2295 and RU486 feeding leads to ddaC neurons showing strong pruning defects in pupae (16h APF) despite of normal dendrite arbors in the white prepupal stage.

      (Wang et al., 2018)

      Expression of Rab1S25N.UAS.YFP under the control of CtxGlia-SplitGal4 (Hsim\VP16AD.nrv2 and Scer\GAL4DBD.wrapper.932i) leads to an altered cortex glial morphology in third instar larvae when compared to controls.

      (Coutinho-Budd et al., 2017)

      When Rab1S25N.Scer\UAS.T:Avic\GFP-YFP is expressed using Scer\GAL4ppk.PG, transgenic animals show defective nociception response.

      (Oswald et al., 2015)

      Expression of Rab1S25N.Scer\UAS.T:Avic\GFP-YFP under the control of Scer\GAL4Ilp2.PW results in a dramatic (>40%) reduction in adult fly weight. Pupal size is also dramatically reduced and flies eclose, on average, ~2 days later than control flies. Hemolymph levels of trehalose and glucose are elevated compared to controls. Fewer Insulin Producing Cells (IPCs) are seen in the larvae and IPC neuronal morphology is dramatically disrupted. Dendritic arborisations are missing while the axonal bundles are mostly intact. Limiting Rab1S25N.Scer\UAS.T:Avic\GFP-YFP expression to late larval development onwards using Scer\GAL80ts.αTub84B avoids the IPC developmental defects.

      (Cao et al., 2014)

      The kinetics of maturation of phagosomes containing pHrodo-conjugated S. aureus by adult hemocytes expressing Rab1S25N.Scer\UAS.T:Avic\GFP-YFP under the control of Scer\GAL4Hml.Δ are not significantly different from those seen in control hemocytes.

      (Garg and Wu, 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhancer of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Rab1S25N.Scer\UAS.T:Avic\GFP-YFP
      Rab1S25N.UAS.YFP
      Name Synonyms
      Secondary FlyBase IDs
        References (16)