FlyBase Allele Report: Dmel\Rab8[Q67L.UASp.YFP]

General Information

Symbol

Dmel\Rab8^{Q67L.UASp.YFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0215403

Feature type

allele

Associated gene

Dmel\Rab8

Associated Insertion(s)

Carried in Construct

P{UASp-YFP.Rab8.Q67L}

Key Links

Transgenic product class

missense_variant

(Zhang et al., 2007)

Mutagen

Nature of the Allele

Transgenic product class

missense_variant

(Zhang et al., 2007)

Mutagen

in vitro construct - amino acid replacement

(Zhang et al., 2007, Zhang et al., 2007)

in vitro construct - regulatory fusion

(Zhang et al., 2007)

in vitro construct - coding region fusion

(Zhang et al., 2007)

Progenitor genotype

Carried in construct

P{UASp-YFP.Rab8.Q67L}

Cytology

Description

Amino acid replacement: Q67L.

(Zhang et al., 2007)

UASp regulatory sequences drive expression of a predicted constitutively active form of Rab8 that is tagged at the N-terminal end with YFP.

(Zhang et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Rab8

Tagged with

YFP

yellow fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Delfino et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Rab8^{Q67L.UASp.YFP}, Scer\GAL4^elav-C155 is a suppressor of increased mortality during development phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Delfino et al., 2020)

Rab8^{Q67L.UASp.YFP}, Scer\GAL4^elav-C155 is a suppressor of short lived phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Delfino et al., 2020)

Rab8^{Q67L.UASp.YFP}/Scer\GAL4^pnr.PU is a suppressor of abnormal cell number | somatic clone | P-stage phenotype of strat^CRISPR

(Bellec et al., 2018)

NOT Suppressor of

Statement

Reference

Rab8^{Q67L.UASp.YFP}/Scer\GAL4^GMR57C10 is a non-suppressor of bang sensitive phenotype of para^bss1

(Kroll et al., 2015)

Rab8^{Q67L.UASp.YFP}/Scer\GAL4^GMR57C10 is a non-suppressor of bang sensitive phenotype of eas²

(Kroll et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

Rab8^{Q67L.UASp.YFP}, Scer\GAL4^elav-C155 is a suppressor of rhabdomere | decreased number phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Delfino et al., 2020)

Rab8^{Q67L.UASp.YFP}, Scer\GAL4^elav-C155 is a suppressor of eye phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Delfino et al., 2020)

Rab8^{Q67L.UASp.YFP}/Scer\GAL4^pnr.PU is a suppressor of sensory neuron | somatic clone | P-stage phenotype of strat^CRISPR

(Bellec et al., 2018)

Rab8^{Q67L.UASp.YFP}, Scer\GAL4^tj.PU is a suppressor | partially of follicle cell phenotype of Scer\GAL4^tj.PU, strat^GD10605

(Devergne et al., 2017)

Rab8^{Q67L.UASp.YFP}, Scer\GAL4^tj.PU is a suppressor | partially of ovarian basement membrane | oogenesis phenotype of Scer\GAL4^tj.PU, strat^GD10605

(Devergne et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of Rab8^{Q67L.Scer\UAS.P\T.T:Avic\GFP-YFP} partially suppresses the basement membrane deposition defects observed in follicle cells expressing strat^GD10605 under the control of Scer\GAL4^tj.PU.

(Devergne et al., 2017)

Expression of Rab8^{Q67L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^GMR57C10 does not rescue the bang sensitivity of para^bss1/+ flies, hemizygous para^bss1 flies or eas² mutants.

(Kroll et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

9781

y¹ w^*; P{UASp-YFP.Rab8.Q67L}05

23301

y¹ w^*; P{UASp-YFP.Rab8.Q67L}Sc2¹⁰/TM3, P{Thb8-lacZ}WD1, Sb¹ Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Rab8^{Q67L.Scer\UAS.P\T.T:Avic\GFP-YFP}

Rab8^{Q67L.UASp.YFP}

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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