FB2024_03 , released June 25, 2024
Allele: Dmel\Frq2UAS.cRPa
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General Information
Symbol
Dmel\Frq2UAS.cRPa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Romero-Pozuelo
FlyBase ID
FBal0217379
Feature type
allele
Associated gene
Dmel\Frq2
Associated Insertion(s)
Carried in Construct
P{UAS-Frq2.RP}
Key Links
Transgenic product class
wild_type
(Romero-Pozuelo et al., 2007)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Romero-Pozuelo et al., 2007)
Mutagen
in vitro construct
(Romero-Pozuelo et al., 2007)
Progenitor genotype
Carried in construct
P{UAS-Frq2.RP}
Cytology
Description

UASt regulatory sequences drive expression of a 750bp fragment containing the Frq2 open reading frame.

(Romero-Pozuelo et al., 2007)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Frq2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Overexpression of Frq2Scer\UAS.cRPa under the control of Scer\GAL4D42 depresses short-term synaptic plasticity in a high Ca[2+] concentration, compared to controls. Expression of Frq2Scer\UAS.cRPa does not change the number of active zones per synapse in 1b boutons nor the number of docked vesicles. These mutants do not show any significant difference in resting Ca[2+] level compared to controls.

      Overexpression of Frq1Scer\UAS.cRPa under the control of Scer\GAL4D42 significantly reduces the number of synaptic boutons.

      Expression of Frq2Scer\UAS.cRPa under the control of Scer\GAL4D42 at 25[o]C results in reduced nerve-terminal morphology.

      (Dason et al., 2009)

      The number of type 1s boutons at the larval neuromuscular junction is reduced compared to wild type in animals expressing Frq2Scer\UAS.cRPa under the control of Scer\GAL4D42, while the number of type 1b boutons is not significantly affected.

      The amplitude of the evoked excitatory junctional potential (EJP) at the neuromuscular junction is increased compared to wild type in larvae expressing Frq2Scer\UAS.cRPa under the control of Scer\GAL4elav-C155 or Scer\GAL4D42. The amplitude and frequency of spontaneous mEJPs is not affected in these flies. Quantal content of type 1b and type 1s boutons is increased in these flies compared to wild type.

      (Romero-Pozuelo et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      Statement
      Reference

      Frq2UAS.cRPa, Scer\GAL4Toll-6-D42 has abnormal neuroanatomy phenotype, non-enhanceable by cac[+]/cacTS2, Scer\GAL4Toll-6-D42

      (Dason et al., 2009)
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      Frq2UAS.cRPa, Scer\GAL4Toll-6-D42 has abnormal neuroanatomy phenotype, non-suppressible by cac[+]/cacTS2, Scer\GAL4Toll-6-D42

      (Dason et al., 2009)
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4Toll-6-D42/Frq2UAS.cRPa is a non-suppressor of abnormal neurophysiology | temperature conditional phenotype of cacTS2

      (Dason et al., 2009)

      Scer\GAL4Toll-6-D42/Frq2UAS.cRPa is a non-suppressor of abnormal neuroanatomy phenotype of cacTS2

      (Dason et al., 2009)
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      Frq2UAS.cRPa, Scer\GAL4Toll-6-D42 has NMJ bouton phenotype, suppressible by cacTS2, Scer\GAL4Toll-6-D42

      (Dason et al., 2009)
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4Toll-6-D42/Frq2UAS.cRPa is a non-suppressor of NMJ bouton phenotype of cacTS2

      (Dason et al., 2009)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of Frq2Scer\UAS.cRPa under the control of Scer\GAL4D42 in a cacTS2 background at 25[o]C results in reduced nerve-terminal morphology.

      Expression of Frq2Scer\UAS.cRPa under the control of Scer\GAL4D42 in a cacTS2 background at 22[o]C does no change the number of synaptic boutons compared to controls.

      The presence of a cacTS2 mutant background suppresses the enhanced neurotransmitter release found upon overexpression of Frq2Scer\UAS.cRPa under the control of Scer\GAL4D42 at both permissive and non-permissive temperatures.

      (Dason et al., 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Frq2Scer\UAS.cRPa
      Frq2UAS.cRPa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Romero-Pozuelo
      Secondary FlyBase IDs
        References (2)