FlyBase Allele Report: Dmel\Fer1HCH[UAS.cMa]

General Information

Symbol

Dmel\Fer1HCH^UAS.cMa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Missirlis

FlyBase ID

FBal0218474

Feature type

allele

Associated gene

Dmel\Fer1HCH

Associated Insertion(s)

Carried in Construct

P{UAS-Fer1HCH.M}

Also Known As

UAS-Fer1HCH

Key Links

Transgenic product class

cDNA

(Missirlis et al., 2007)

wild_type

(Missirlis et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Missirlis et al., 2007)

wild_type

(Missirlis et al., 2007)

Mutagen

in vitro construct

(Missirlis et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-Fer1HCH.M}

Cytology

Description

UASt regulatory sequences drive expression of the Fer1HCH LD03437 cDNA (without the iron response element of the 5' UTR).

(Missirlis et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Fer1HCH

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Friedreich ataxia

modeled by fh¹

(Chen et al., 2016)

modeled by fh^RNAi.UAS.cLa

(Navarro et al., 2015)

ameliorates Parkinson's disease 6

modeled by Pink1^B9

(Xia et al., 2024)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm, with Scer\GAL4^nrv2.PS

(Kosmidis et al., 2011)

abnormal circadian rhythm, with Scer\GAL4^repo

(Kosmidis et al., 2011)

abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4^elav-C155

(Kosmidis et al., 2014)

Phenotype Manifest In

Detailed Description

Statement

Reference

The performance of young flies expressing Scer\GAL4^elav-C155>Fer1HCH^Scer\UAS.cMa in negative geotactic response is similar to wild-type. They transgenic flies show significant impairment when age-related decline starts at week six of their life.

Overexpression of Scer\GAL4^elav-C155>Fer1HCH^Scer\UAS.cMa results in behavioural defects, neurodegeneration, and reduced life span only when mothers are infected with virulent Wolbachia. Treatment of flies with the antibiotic tetracycline for four consecutive generations results in the elimination of these phenotypes.

(Kosmidis et al., 2014)

Fer1HCH^Scer\UAS.cMa overexpression in all adult brain glia under the control of Scer\GAL4^repo does not generate statistically significant differences in escape response in the first four weeks of adult life.

Approximately 75% of flies overexpressing Fer1HCH^Scer\UAS.cMa in glia under the control of Scer\GAL4^repo exhibit rhythmicity, while 80% of those expressing Fer1HCH^Scer\UAS.cMa under the control of Scer\GAL4^nrv2.PS exhibit rhythmicity.

(Kosmidis et al., 2011)

Expression of Fer1HCH^Scer\UAS.cMa alone under the control of Scer\GAL4^Act does not result in increased resistance to paraquat compared to control flies.

(Missirlis et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Fer1HCH^UAS.cMa, Scer\GAL4^elav-C155 has abnormal locomotor behavior | adult stage | progressive phenotype, enhanceable by Fer2LCH^UAS.cMa, Scer\GAL4^elav-C155

(Kosmidis et al., 2014)

Fer1HCH^UAS.cMa, Scer\GAL4^repo has abnormal circadian rhythm phenotype, enhanceable by Fer2LCH^UAS.cMa, Scer\GAL4^repo

(Kosmidis et al., 2011)

Enhancer of

Statement

Reference

Fer1HCH^UAS.cMa, Scer\GAL4^repo is an enhancer of abnormal circadian rhythm phenotype of Fer2LCH^UAS.cMa, Scer\GAL4^repo

(Kosmidis et al., 2011)

Suppressor of

Statement

Reference

Scer\GAL4^ple.PF/Fer1HCH^UAS.cMa is a suppressor | partially of decreased rate of adult locomotory behavior phenotype of Pink1^B9

(Xia et al., 2024)

Scer\GAL4^ple.PF/Fer1HCH^UAS.cMa is a suppressor of decreased rate of jump response phenotype of Pink1^B9

(Xia et al., 2024)

Scer\GAL4^ple.PF/Fer1HCH^UAS.cMa is a suppressor of abnormal neuroanatomy | adult stage phenotype of Pink1^B9

(Xia et al., 2024)

Scer\GAL4^ninaE.PT, Fer2LCH^UAS.cMa, Fer1HCH^UAS.cMa is a suppressor | partially of abnormal neurophysiology | somatic clone | adult stage phenotype of fh¹

(Chen et al., 2016)

Fer2LCH^UAS.cMa/Fer1HCH^UAS.cMa, Scer\GAL4^Act5C.PU is a suppressor | partially of chemical sensitive phenotype of Scer\GAL4^Act5C.PU, fh^RNAi.UAS.cLa

(Navarro et al., 2015)

Scer\GAL4^Act5C.PU, Fer2LCH^UAS.cMa, Fer1HCH^UAS.cMa is a suppressor of short lived | nutrition conditional phenotype of fh^RNAi.UAS.cLa

(Navarro et al., 2015)

Scer\GAL4^Act5C.PU, Fer2LCH^UAS.cMa, Fer1HCH^UAS.cMa is a suppressor of short lived phenotype of fh^RNAi.UAS.cLa

(Navarro et al., 2015)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Act.PU/Fer1HCH^UAS.cMa is a non-suppressor of lethal | recessive phenotype of Fer2LCH⁰⁰⁰³⁵

(Missirlis et al., 2007)

Other

Statement

Reference

Fer1HCH^UAS.cMa, Fer2LCH^UAS.cMa, Scer\GAL4^Act.PU has chemical resistant | female limited phenotype

(Missirlis et al., 2007)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^ple.PF/Fer1HCH^UAS.cMa is a suppressor of adult brain phenotype of Pink1^B9

(Xia et al., 2024)

Scer\GAL4^ple.PF/Fer1HCH^UAS.cMa is a suppressor of adult dopaminergic neuron | decreased number phenotype of Pink1^B9

(Xia et al., 2024)

Scer\GAL4^ninaE.PT, Fer2LCH^UAS.cMa, Fer1HCH^UAS.cMa is a suppressor | partially of eye photoreceptor cell | somatic clone | adult stage phenotype of fh¹

(Chen et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of both Fer1HCH^Scer\UAS.cMa and Fer2LCH^Scer\UAS.cMa under the control of Scer\GAL4^ninaE.PT significantly rescues the neurodegeneration and electrophysiology defect seen in fh¹ mutant photoreceptor clones when flies are raised in the dark.

(Chen et al., 2016)

Co-expression of Fer1HCH^Scer\UAS.cMa and Fer2LCH^Scer\UAS.cMa partially suppresses the reduction in lifespan seen in flies expressing fh^{dsRNA.Scer\UAS.cLa} under the control of Scer\GAL4^Act5C.PU when fed either normal food or food supplemented with iron.

(Navarro et al., 2015)

Flies overexpressing both Fer1HCH^Scer\UAS.cMa and Fer2LCH^Scer\UAS.cMa under the control of Scer\GAL4^elav-C155 show significant impairment in negative geotaxis at file and six weeks of age.

(Kosmidis et al., 2014)

Flies simultaneously expressing Fer1HCH^Scer\UAS.cMa and Fer2LCH^Scer\UAS.cMa in glia under the control of Scer\GAL4^repo exhibit an abrupt and pronounced deficit in escape responses in both horizontal and vertical directions at five and six weeks of age.

Quantification of rhythmic patterns shows that 57% of six-week old individuals overexpressing both Fer1HCH^Scer\UAS.cMa and Fer2LCH^Scer\UAS.cMa in glia under the control of Scer\GAL4^repo remain rhythmic in constant darkness, albeit with a reduced value for rhythmicity statistic, suggesting behavioral disturbances even in the flies categorized as rhythmic. Three-week old flies overexpressing both chains in glia show robut rhythms in 69% of the individuals tested. Approximately 63% of six-week old Scer\GAL4^nrv2.PS/Fer1HCH^Scer\UAS.cMa, Fer2LCH^Scer\UAS.cMa flies are rhythmic compared to 91% for similar aged Scer\GAL4^nrv2.PS/+ flies.

Flies overexpressing both Fer1HCH^Scer\UAS.cMa and Fer2LCH^Scer\UAS.cMa in glia under the control of Scer\GAL4^repo or Scer\GAL4^nrv2.PS accumulate ferritin-iron inclusions, with crystal-like (i.e. symmetric) morphology and are surrounded by an intracellular membrane.

Flies overexpressing both Fer1HCH^Scer\UAS.cMa and Fer2LCH^Scer\UAS.cMa in glia under the control of Scer\GAL4^repo or Scer\GAL4^nrv2.PS do not exhibit any apparent neurodegeneration or a shorter lifespan compared to controls.

(Kosmidis et al., 2011)

Co-expression of both Fer1HCH^Scer\UAS.cMa and Fer2LCH^Scer\UAS.cMa under the control of Scer\GAL4^Act results in females with greater resistance to paraquat than wild-type controls (males show the same sensitivity to paraquat as controls).

Males co-expressing both Fer1HCH^Scer\UAS.cMa and Fer2LCH^Scer\UAS.cMa under the control of Scer\GAL4^Act are at a developmental disadvantage compared to their control siblings, with this effect being most pronounced under iron-limiting conditions and with stronger overexpression of Fer1HCH^Scer\UAS.cMa and Fer2LCH^Scer\UAS.cMa.

(Missirlis et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^Act.PU/Fer1HCH^UAS.cMa rescues Fer1HCH⁰⁰⁴⁵¹

(Missirlis et al., 2007)

Scer\GAL4^Act.PU/Fer1HCH^UAS.cMa rescues Fer1HCH^G00188/Fer1HCH⁰⁰⁴⁵¹

(Missirlis et al., 2007)

Comments

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