FlyBase Allele Report: Dmel\gwl[Sr6]

General Information

Symbol

Dmel\gwl^Sr6

Species

D. melanogaster

Name

FlyBase ID

FBal0218849

Feature type

allele

Associated gene

Dmel\gwl

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

X ray

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Archambault et al., 2007)

Mutagen

X ray

(Archambault et al., 2007)

Progenitor genotype

Cytology

Description

In addition to the amino acid substitution, gwl^Sr6 has a K689STOP substitution.

(Archambault et al., 2007)

Nucleotide substitution: A?T.

(Archambault et al., 2007)

Amino acid replacement: K97M.

(Archambault et al., 2007)

Amino acid replacement: K689term.

(Archambault et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:18,749,461..18,749,461 [+]

Nucleotide change:

A18749461T

Reported nucleotide change:

A?T

Amino acid change:

K690term | gwl-PA; K647term | gwl-PB

Reported amino acid change:

K689term

(Archambault et al., 2007)

point_mutation

3R:18,747,376..18,747,376 [+]

Nucleotide change:

A18747376T

Reported nucleotide change:

A?T

Amino acid change:

K97M | gwl-PA; K97M | gwl-PB

Reported amino acid change:

K97M

(Archambault et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eclosion rhythm (with gwl^Sr3)

(Archambault et al., 2007)

abnormal mitotic cell cycle (with gwl^Sr3)

(Archambault et al., 2007)

female fertile (with gwl²)

(Archambault et al., 2007)

female fertile (with gwl^3a)

(Archambault et al., 2007)

female fertile (with gwl^6a)

(Archambault et al., 2007)

female sterile (with gwl^Sr3), with Scer\GAL4^Act.PU, gwl^{NLSmut.UASp.Tag:MYC}

(Wang et al., 2013)

fertile (with gwl²)

(Archambault et al., 2007)

fertile (with gwl^3a)

(Archambault et al., 2007)

fertile (with gwl^6a)

(Archambault et al., 2007)

lethal (with gwl^Sr3)

(Wang et al., 2013)

partially lethal - majority live (with gwl^Sr3)

(Archambault et al., 2007)

sterile

(Archambault et al., 2007)

viable (with gwl^Sr3), with Scer\GAL4^Act.PU, gwl^{NLSmut.UASp.Tag:MYC}

(Wang et al., 2013)

Phenotype Manifest In

chromatin

(Archambault et al., 2007)

chromatin (with gwl^Sr3)

(Archambault et al., 2007)

eye (with gwl^Sr3)

(Archambault et al., 2007)

gonad (with gwl^Sr3)

(Archambault et al., 2007)

macrochaeta (with gwl^Sr3)

(Archambault et al., 2007)

ovary (with gwl^Sr3), with Scer\GAL4^Act.PU, gwl^{NLSmut.UASp.Tag:MYC}

(Wang et al., 2013)

wing margin (with gwl^Sr3)

(Archambault et al., 2007)

Detailed Description

Statement

Reference

Scer\GAL4^Act.PU-mediated expression of gwl^{NLSmut.Scer\UAS.P\T.T:Hsap\MYC} in a gwl^Sr3/gwl^Sr6 mutants background results in viable but female sterile flies. Ovaries are completely lacking.

(Wang et al., 2013)

gwl^Sr6 homozygotes die as pupae (although this is believed to reflect a second lethal on its chromosome).

gwl^Sr6 mutants are sterile in both males and females.

gwl^Sr3/gwl^6a mutants exhibit mitotic defects, leading to an increase in the mitotic index with more cells in anaphase. Mutant neuroblasts show a high frequency of defective chromatin condensation. Some regions of chromatin appear undercondensed and other regions are overcondensed. In some cells the chromosomes are uniformly overcondensed, suggesting a lengthy pre-anaphase delay.

gwl^Sr3/gwl^Sr6 adults eclose (after a delay), with variably rough eyes, eroded wing margins, missing bristles and strongly reduced gonads, though viability is reduced.

gwl^Sr6/Df(3L)gwl^3b mutants die predominantly in the pupal stage, and the rare escaper adults have rough eyes, ragged wings, cuticle defects, missing bristles, and are sterile.

gwl^3a/gwl^Sr6 mutants produce a gwl^Sr3 phenotype, i.e female fertility is restored.

gwl²/gwl^Sr6 mutants produce a gwl^Sr3 phenotype, i.e female fertility is restored.

gwl^6a/gwl^Sr6 mutants produce a gwl^Sr3 phenotype, i.e female fertility is restored.

(Archambault et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

gwl^Sr6/gwl^Sr3 is rescued by Scer\GAL4^Act.PU/gwl^UASp.Tag:MYC

(Wang et al., 2013)

gwl^Sr6/gwl^Sr3 is rescued by Scer\GAL4^Act5C.PU/gwl^L.UASp

(Archambault et al., 2007)

gwl^Sr6/gwl^Sr3 is rescued by Scer\GAL4^Act5C.PU/gwl^K97M.UASp

(Archambault et al., 2007)

gwl^Sr6/Df(3L)gwl^3b is rescued by Scer\GAL4^Act5C.PU/gwl^L.UASp

(Archambault et al., 2007)

gwl^Sr6/Df(3L)gwl^3b is rescued by Scer\GAL4^Act5C.PU/gwl^K97M.UASp

(Archambault et al., 2007)

Partially rescued by

gwl^Sr6/gwl^Sr3 is partially rescued by gwl^{NLSmut.UASp.Tag:MYC}/Scer\GAL4^Act.PU

(Wang et al., 2013)

gwl^Sr6/gwl^Sr3 is partially rescued by Scer\GAL4^Act.PU/gwl^{UASp.Tag:NLS(SV40-largeT).Tag:MYC}

(Wang et al., 2013)

Comments

Scer\GAL4^Act.PU-mediated expression of gwl^{Scer\UAS.P\T.T:Hsap\MYC} completely rescues the lethality of gwl^Sr3/gwl^Sr6 mutants.

Scer\GAL4^Act.PU-mediated expression of gwl^{NLSmut.Scer\UAS.P\T.T:Hsap\MYC} incompletely rescues the lethality of gwl^Sr3/gwl^Sr6 mutants.

Scer\GAL4^Act.PU-mediated expression of gwl^{Scer\UAS.P\T.T:SV40\nls2.T:Hsap\MYC} incompletely rescues the lethality of gwl^Sr3/gwl^Sr6 mutants.

(Wang et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Based on viability, the following gwl alleles can be ranked from strongest to weakest as follows: gwl^Sr3 > gwl^Sr3/Df(3L)gwl^3b > gwl^Sr6 > gwl^Sr6/Df(3L)gwl^3b> gwl^Sr3/gwl^Sr6 > gwl^Sr18/Df(3L)gwl^3b.

(Archambault et al., 2007)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

gwl^Sr6

(Wang et al., 2013, Archambault et al., 2007)

Name Synonyms

Scant revertant

(Archambault et al., 2007)

Secondary FlyBase IDs

References (2)

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