FlyBase Allele Report: Dmel\gwl[L.UASp]

General Information

Symbol

Dmel\gwl^L.UASp

Species

D. melanogaster

Name

UAS construct of the Long isoform of greatwall

FlyBase ID

FBal0218854

Feature type

allele

Associated gene

Dmel\gwl

Associated Insertion(s)

Carried in Construct

P{UASp-gwl.Long}

Key Links

Transgenic product class

wild_type

(Archambault et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Archambault et al., 2007)

Mutagen

in vitro construct

(Archambault et al., 2007)

Progenitor genotype

Carried in construct

P{UASp-gwl.Long}

Cytology

Description

UASp regulatory sequences drive expression of the gwl coding sequences corresponding to the long isoform (required for female meiosis).

(Archambault et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

gwl

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Female germline expression of gwl^L.Scer\UAS (under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}) has no effect on fertility, with embryos appearing wild-type.

(Archambault et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

gwl^L.UASp/Scer\GAL4^{VP16.mat.αTub67C} is a suppressor of sterile phenotype of polo¹¹

(Archambault et al., 2007)

Other

Statement

Reference

Scer\GAL4^{VP16.mat.αTub67C}, gwl^L.UASp, tws^j11C8 has abnormal meiotic cell cycle | dominant phenotype

(Wang et al., 2011)

Scer\GAL4^{VP16.mat.αTub67C}, gwl^L.UASp, tws^j11C8 has sterile | dominant phenotype

(Wang et al., 2011)

Phenotype Manifest In

Enhancer of

Statement

Reference

gwl^L.UASp/Scer\GAL4^{VP16.mat.αTub67C} is an enhancer of centrosome phenotype of polo¹¹

(Archambault et al., 2007)

Suppressor of

Statement

Reference

gwl^L.UASp/Scer\GAL4^{VP16.mat.αTub67C} is a suppressor of egg phenotype of polo¹¹

(Archambault et al., 2007)

gwl^L.UASp/Scer\GAL4^{VP16.mat.αTub67C} is a suppressor of spindle phenotype of polo¹¹

(Archambault et al., 2007)

Other

Statement

Reference

Scer\GAL4^{VP16.mat.αTub67C}, gwl^L.UASp, tws^j11C8 has spindle | embryonic stage phenotype

(Wang et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Overexpression of gwl^L.Scer\UAS in eggs/embryos under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} from tws^j11C8/+ mothers is almost completely lethal. Most eggs/embryos are blocked in metaphase of meiosis I, even after 4 to 6 hours post-laying. Only a few eggs manage to complete meiosis and attempt to initiate embryonic development. However, these embryos usually abort in the first mitosis, displaying several aberrant structures with condensed chromatin at the center of small spindles. The few mitotic nuclei observed show a very high incidence of detached centrosomes.

(Wang et al., 2011)

Female germline expression of gwl^L.Scer\UAS (under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}) in polo¹¹ heterozygous females allows partial egg hatch (around 4%), and these embryos also show loss of centrosomes from spindles in early syncytial divisions.

(Archambault et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Comments

Expression of gwl^L.Scer\UAS under the control of Scer\GAL4^Act5C rescues the viability and somatic integrity of gwl^Sr3/gwl^Sr6, gwl^Sr3/gwl^Sr3, gwl^Sr3/Df(3L)gwl^3b and gwl^Sr3/Df(3L)gwl^3b flies to similar degrees.

The fertility of gwl^Sr18/Df(3L)gwl^3b females is rescued completely by expression of gwl^L.Scer\UAS in the maternal germline with Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}.

(Archambault et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

gwl^L.Scer\UAS

gwl^L.UASp

Name Synonyms

UAS construct of the Long isoform of greatwall

Secondary FlyBase IDs

References (2)

Report Sections

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