FB2024_03 , released June 25, 2024
Allele: Dmel\gwlK97M.UASp
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General Information
Symbol
Dmel\gwlK97M.UASp
Species
D. melanogaster
Name
FlyBase ID
FBal0218855
Feature type
allele
Associated gene
Dmel\gwl
Associated Insertion(s)
Carried in Construct
P{UASp-gwl.K97M}
Key Links
Transgenic product class
missense_variant
(Archambault et al., 2007)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Archambault et al., 2007)
Mutagen
in vitro construct
(Archambault et al., 2007)
Progenitor genotype
Carried in construct
P{UASp-gwl.K97M}
Cytology
Description

Amino acid replacement: K97M.

(Archambault et al., 2007)

UASp regulatory sequences drive expression of mutated gwl coding sequences.

(Archambault et al., 2007)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
gwl
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Female germline expression of gwlK97M.Scer\UAS (under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16) reduces fertility (t0 23%), with some centrosome loss usually occurring in the later divisions of the syncytial blastoderm stage.

      (Archambault et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Statement
      Reference

      gwlK97M.UASp/Scer\GAL4VP16.mat.αTub67C is an enhancer of sterile phenotype of polo11

      (Archambault et al., 2007)
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      gwlK97M.UASp/Scer\GAL4VP16.mat.αTub67C is an enhancer of spindle phenotype of polo11

      (Archambault et al., 2007)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Female germline expression of gwlK97M.Scer\UAS (under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16) in polo11 heterozygotes causes complete sterility. These embryos show loss of centrosomes from very early mitotic spindles.

      (Archambault et al., 2007)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      gwlK97M.Scer\UAS
      gwlK97M.UASp
      Name Synonyms
      UAS construct of the Long isoform of greatwall
      Secondary FlyBase IDs
        References (1)