FlyBase Allele Report: Dmel\TkR99D[MB09356]

General Information

Symbol

Dmel\TkR99D^MB09356

Species

D. melanogaster

Name

FlyBase ID

FBal0219519

Feature type

allele

Associated gene

Dmel\TkR99D

Associated Insertion(s)

Mi{ET1}TkR99D^MB09356

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

Mi{ET1}TkR99D^MB09356

Cytology

Description

Allele components

Component

Use(s)

Inserted element

Mi{ET1}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:29,956,652..29,956,652 [+]

(Asahina et al., 2014, Bellen, 2008.12.12)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

larval multidendritic class IV neuron | third instar larval stage | conditional (with TkR99D^f02797)

(Im et al., 2015)

Detailed Description

Statement

Reference

TkR99D^MB09356/TkR99D^MB09356 early third instar larvae do not have significantly different baseline thermal nociception (uninjured larval responses to thermal probes at 45[o] or 48[o]C) compared to controls. 24 hours after UV-induced nociceptive sensitization, early third instar TkR99D^MB09356/TkR99D^MB09356, TkR99D^f02797/TkR99D^MB09356, TkR99D^MB09356/Df(3R)Exel6213 or TkR99D^MB09356/TkR99D^s2222 larvae show significant reduction of thermal allodynia (withdrawal responses to a sub-threshold 38[o]C probe) compared to wild type or heterozygote larvae.

TkR99D^f02797/TkR99D^MB09356 third instar larval fillets do not show a UV-induced increase in action potential firing rate at higher temperatures (36-39[o]C) in class IV neurons, as seen in controls.

(Im et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

TkR99D^MB09356 is a non-suppressor of abnormal behavior | adult stage | heat sensitive phenotype of Scer\GAL4^Tk, TrpA1^UAS.(B).cKa

(Asahina et al., 2014)

Other

Statement

Reference

TkR86C^ΔF28, TkR99D^MB09356 has abnormal behavior | adult stage phenotype

(Asahina et al., 2014)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Homozygous TkR99D^MB09356 does not suppress the aggression-promoting effect of activating neurons labeled with Scer\GAL4^Tk (either the P{Tk-GAL4.TH}5Fa or P{Tk-GAL4.TH}3Ma insertion) through expression of thermosensitive TrpA1^{Scer\UAS.(B).cKa}.

TkR86C^ΔF28, TkR99D^MB09356 double mutants show a strong reduction in baseline and induced aggression compared to controls.

(Asahina et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

TkR99D^f02797/TkR99D^MB09356 is rescued by Scer\GAL4^ppk.1.9/TkR99D^UAS.EGFP

(Im et al., 2015)

Comments

Expression of TkR99D^{Scer\UAS.T:Avic\GFP-EGFP} driven by Scer\GAL4^ppk.1.9 significantly rescues the reduction of thermal allodynia (withdrawal responses to a sub-threshold 38[o]C probe after UV-induced nociceptive sensitization) seen in early third instar TkR99D^f02797/TkR99D^MB09356 larvae. TkR99D^{Scer\UAS.T:Avic\GFP-EGFP} driven by Scer\GAL4^ppk.1.9 significantly restores a UV-induced increase in action potential firing rate in class IV neurons at higher temperatures (36-39[o]C) in third instar TkR99D^f02797/TkR99D^MB09356 larval fillets.

(Im et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

26471

w¹¹¹⁸; Mi{ET1}TkR99D^MB09356

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

TAKR^MB09356

(Qi et al., 2021)

Takr99D^MB09356

(Asahina et al., 2014, Bellen, 2008.12.12)

TkR99D^MB09356

dtkr^MB09356

(Im et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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