FlyBase Allele Report: Dmel\TkR99D[UAS.EGFP]

General Information

Symbol

Dmel\TkR99D^UAS.EGFP

Species

D. melanogaster

Name

FlyBase ID

FBal0287946

Feature type

allele

Associated gene

Dmel\TkR99D

Associated Insertion(s)

Carried in Construct

P{UAS-TkR99D.GFP}

Also Known As

UAS-dtkr-GFP

Key Links

Transgenic product class

wild_type

(Ignell et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ignell et al., 2009)

Mutagen

in vitro construct

(Ignell et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-TkR99D.GFP}

Cytology

Description

UASt regulates expression of TkR99D fused to EGFP.

(Ignell et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

TkR99D

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | third instar larval stage | heat sensitive, with Scer\GAL4^ppk.1.9

(Im et al., 2015)

abnormal pain response | early third instar larval stage | heat sensitive, with Scer\GAL4^ppk.1.9

(Im et al., 2015)

abnormal smell perception, with Scer\GAL4^{Orco.2.642.Hsim\VP22}

(Ignell et al., 2009)

Phenotype Manifest In

larval multidendritic class IV neuron | third instar larval stage | heat sensitive, with Scer\GAL4^ppk.1.9

(Im et al., 2015)

Detailed Description

Statement

Reference

Compared to controls unresponsive to a 34, 36 or 38[o]C probes (without pre-exposure to UV), early third instar larvae with TkR99D^{Scer\UAS.T:Avic\GFP-EGFP} over-expressed by Scer\GAL4^ppk.1.9 show significant withdrawal responses.

Third instar larval fillets with TkR99D^{Scer\UAS.T:Avic\GFP-EGFP} driven by Scer\GAL4^ppk.1.9 show significant increases in action potential firing rates in response to thermal stimuli in class IV neurons at higher temperatures (36-40[o]C, without UV exposure; similar to UV-treated controls in magnitude).

(Im et al., 2015)

Scer\GAL4^{Orco.2.642.T:Hsim\VP22}-mediated expression of TkR99D^{Scer\UAS.T:Avic\GFP-EGFP} results in a significant decrease in behavioural response index at high concentrations of ethyl-3-hydroxybutyrate and methyl hexanoate.

(Ignell et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^ppk.1.9, TkR99D^UAS.EGFP has abnormal pain response | early third instar larval stage | heat sensitive phenotype, suppressible | partially by CG17760^GD17286, Scer\GAL4^ppk.1.9

(Im et al., 2015)

Scer\GAL4^ppk.1.9, TkR99D^UAS.EGFP has abnormal pain response | early third instar larval stage | heat sensitive phenotype, suppressible | partially by CG17760^KK113244, Scer\GAL4^ppk.1.9

(Im et al., 2015)

Scer\GAL4^ppk.1.9, TkR99D^UAS.EGFP has abnormal pain response | early third instar larval stage | heat sensitive phenotype, suppressible | partially by pain^GD2221/Scer\GAL4^ppk.1.9

(Im et al., 2015)

Scer\GAL4^ppk.1.9, TkR99D^UAS.EGFP has abnormal pain response | early third instar larval stage | heat sensitive phenotype, suppressible | partially by pain^JF01065/Scer\GAL4^ppk.1.9

(Im et al., 2015)

Scer\GAL4^ppk.1.9, TkR99D^UAS.EGFP has abnormal pain response | early third instar larval stage | heat sensitive phenotype, suppressible | partially by pain⁷⁰/Scer\GAL4^ppk.1.9

(Im et al., 2015)

Scer\GAL4^ppk.1.9, TkR99D^UAS.EGFP has abnormal pain response | early third instar larval stage | heat sensitive phenotype, suppressible | partially by disp^GD2813/Scer\GAL4^ppk.1.9

(Im et al., 2015)

Scer\GAL4^ppk.1.9, TkR99D^UAS.EGFP has abnormal pain response | early third instar larval stage | heat sensitive phenotype, suppressible | partially by Gβ5^JF02941, Scer\GAL4^ppk.1.9

(Im et al., 2015)

Scer\GAL4^ppk.1.9, TkR99D^UAS.EGFP has abnormal pain response | early third instar larval stage | heat sensitive phenotype, suppressible | partially by Gβ5^KK108311, Scer\GAL4^ppk.1.9

(Im et al., 2015)

Scer\GAL4^ppk.1.9, TkR99D^UAS.EGFP has abnormal pain response | early third instar larval stage | heat sensitive phenotype, suppressible | partially by Gγ1^HMS01361, Scer\GAL4^ppk.1.9

(Im et al., 2015)

Scer\GAL4^ppk.1.9, TkR99D^UAS.EGFP has abnormal pain response | early third instar larval stage | heat sensitive phenotype, suppressible | partially by Gγ1^NIG.8261R, Scer\GAL4^ppk.1.9

(Im et al., 2015)

Scer\GAL4^ppk.1.9, TkR99D^UAS.EGFP has abnormal pain response | early third instar larval stage | heat sensitive phenotype, suppressible | partially by ptc^UAS.cJa/Scer\GAL4^ppk.1.9

(Im et al., 2015)

Scer\GAL4^ppk.1.9, TkR99D^UAS.EGFP has abnormal pain response | early third instar larval stage | heat sensitive phenotype, suppressible | partially by smo^{5A.UAS.Tag:FLAG}/Scer\GAL4^ppk.1.9

(Im et al., 2015)

Scer\GAL4^ppk.1.9, TkR99D^UAS.EGFP has abnormal pain response | early third instar larval stage | heat sensitive phenotype, suppressible | partially by ci^76.UAS/Scer\GAL4^ppk.1.9

(Im et al., 2015)

Scer\GAL4^ppk.1.9, TkR99D^UAS.EGFP has abnormal pain response | early third instar larval stage | heat sensitive phenotype, suppressible by en^KK107874/Scer\GAL4^ppk.1.9

(Im et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of CG17760^GD17286, CG17760^KK113244, Gβ5^JF02941, Gβ5^KK108311, Gγ1^HMS01361, Gγ1^NIG.8261R, ptc^Scer\UAS.cJa, smo^{5A.Scer\UAS.T:Zzzz\FLAG}, ci^76.Scer\UAS, en^KK107874, pain^GD2221, pain^JF01065, disp^GD2813 or presence of pain⁷⁰/pain⁷⁰ (but not pain⁷⁰/+) significantly suppresses the withdrawal responses to a 38[o]C probe (without pre-exposure to UV) seen in early third instar larvae with TkR99D^{Scer\UAS.T:Avic\GFP-EGFP} over-expressed by Scer\GAL4^ppk.1.9.

(Im et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^ppk.1.9/TkR99D^UAS.EGFP rescues TkR99D^GD375

(Im et al., 2015)

Scer\GAL4^ppk.1.9/TkR99D^UAS.EGFP rescues TkR99D^f02797/TkR99D^MB09356

(Im et al., 2015)

Comments

Co-expression of TkR99D^{Scer\UAS.T:Avic\GFP-EGFP} significantly rescues the reduction of thermal allodynia (withdrawal responses to a sub-threshold 38[o]C probe after UV-induced nociceptive sensitization) seen in early third instar larvae with TkR99D^GD375 driven by Scer\GAL4^ppk.1.9. Expression of TkR99D^{Scer\UAS.T:Avic\GFP-EGFP} driven by Scer\GAL4^ppk.1.9 significantly rescues the reduction of thermal allodynia (withdrawal responses to a sub-threshold 38[o]C probe after UV-induced nociceptive sensitization) seen in early third instar TkR99D^f02797/TkR99D^MB09356 larvae.

TkR99D^{Scer\UAS.T:Avic\GFP-EGFP} driven by Scer\GAL4^ppk.1.9 significantly restores a UV-induced increase in action potential firing rate in class IV neurons at higher temperatures (36-39[o]C) in third instar TkR99D^f02797/TkR99D^MB09356 larval fillets.

(Im et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

TkR99D^{Scer\UAS.T:Avic\GFP-EGFP}

TkR99D^UAS.EGFP

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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