FB2024_03 , released June 25, 2024
Allele: Dmel\Ir84aUAS.cBa
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General Information
Symbol
Dmel\Ir84aUAS.cBa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Benton
FlyBase ID
FBal0220387
Feature type
allele
Associated gene
Dmel\Ir84a
Associated Insertion(s)
Carried in Construct
P{UAS-Ir84a.B}
Also Known As
UAS-IR84a
Key Links
Transgenic product class
wild_type
(Benton et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Benton et al., 2009)
Mutagen
in vitro construct
(Benton et al., 2009)
Progenitor genotype
Carried in construct
P{UAS-Ir84a.B}
Cytology
Description

UASt regulatory sequences drive expression of the full length Ir84a open reading frame.

(Benton et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Ir84a
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing Ir84aScer\UAS.cBa under the control of Scer\GAL4Or35a.PF results in a strong olfactory response to phenylacetaldehyde in the ac3 sensilla (a response not seen in the wild-type ac3 sensilla), although a response to phenylacetonitrile is not seen in the ac3 sensilla in these flies.

      (Benton et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Neurons co-expressing Ir84aScer\UAS.cBa and Ir8aScer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP under the control of Scer\GAL4Or22a.7.717 respond to phenylacetaldehyde in a concentration-dependent manner.

      Neurons co-expressing Ir84aScer\UAS.cBa and Ir8aΔATD.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP under the control of Scer\GAL4Or22a.7.717 fail to respond to phenylacetaldehyde.

      Neurons co-expressing Ir8aΔC.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP and Ir8aScer\UAS.cAa under the control of Scer\GAL4Or22a.7.717 fail to respond to phenylacetaldehyde.

      Neurons co-expressing Ir84aScer\UAS.cBa and Ir8aR481A.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP under the control of Scer\GAL4Or22a.7.717 respond to phenylacetaldehyde in a concentration-dependent manner.

      Neurons co-expressing Ir84aScer\UAS.cBa and Ir8aR481E.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP under the control of Scer\GAL4Or22a.7.717 show a moderate but significant reduction in phenylacetaldehyde responses.

      Neurons co-expressing Ir84aScer\UAS.cBa and Ir8aT645A.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP under the control of Scer\GAL4Or22a.7.717 respond to phenylacetaldehyde in a concentration-dependent manner.

      Neurons co-expressing Ir84aScer\UAS.cBa and Ir8aD724A.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP under the control of Scer\GAL4Or22a.7.717 fail to respond to phenylacetaldehyde.

      (Abuin et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4Ir84a-GAL4/Ir84aUAS.cBa rescues Ir84aGAL4

      (Grosjean et al., 2011)
      Partially rescues

      Scer\GAL4Ir84a-GAL4/Ir84aUAS.cBa partially rescues Ir84aGAL4

      (Grosjean et al., 2011)
      Comments

      Expression of Ir84aScer\UAS.cBa using Scer\GAL4Ir84a-GAL4 restores the response to phenylacetic acid and phenylacetaldehyde in Ir84aGAL4 homozygotes. The reduced courtship seen in Ir84aGAL4 males is also rescued.

      The increased courtship seen in wild type males when killed female objects are perfumed with phenylacetic acid (or fly food) is not observed in Ir84aGAL4 males, but this is partially restored by expression of Ir84aScer\UAS.cBa.

      (Grosjean et al., 2011)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Generated using the phiC31-based integration system.

      (Benton et al., 2009)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Ir84aScer\UAS.cBa
      Ir84aUAS.cBa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Benton
      Secondary FlyBase IDs
        References (6)