FB2024_03 , released June 25, 2024
Allele: Dmel\Vps4DN.UAS.Tag:HA
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General Information
Symbol
Dmel\Vps4DN.UAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0221338
Feature type
allele
Associated gene
Dmel\Vps4
Associated Insertion(s)
Carried in Construct
P{UAS-Vps4.DN-HA}
Also Known As
UAS-Vps4DN
Key Links
Transgenic product class
dominant_negative_variant
(Rusten et al., 2007)
missense_variant
(Rusten et al., 2007)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Rusten et al., 2007)
missense_variant
(Rusten et al., 2007)
Mutagen
in vitro construct
(Rusten et al., 2007)
Progenitor genotype
Carried in construct
P{UAS-Vps4.DN-HA}
Cytology
Description

Amino acid replacement: E232Q.

(Rusten et al., 2007)

UASt regulatory sequences drive expression of a dominant negative form of Vps4 that is tagged at the C-terminal end with Tag:HA.

(Rusten et al., 2007)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Vps4
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expressing Vps4DN.UAS.Tag:HA under the control of Scer\GAL4ppk.PU induces enlarged endosomes but does not affect mitochondria localization in white prepupal ddaC neurons' cell bodies, as compared to controls.

      (Zong et al., 2018)

      Expression of Vps4DN.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4hs.PB using heat shock results in a disruption of the actin cytoskeleton in egg chambers, with tangles of abnormal actin accumulation.

      Larvae expressing Vps4DN.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Bx-MS1096-KE continue to grow beyond the normal time of growth termination, become oversized and die shortly after delayed pupation.

      Wing discs expressing Vps4DN.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Bx-MS1096-KE form enlarged tubular structures which contain internal vesicles when characterised ultrastructurally (these structures are not seen in control discs). These structures are often irregular in shape and sometimes occupy most of the cell volume. An increased number of apoptotic cells are seen in these wing discs compared to controls.

      Expression of Vps4DN.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Bx-MS1096-KE results in disruption of the actin cytoskeleton in the wing imaginal disc.

      Wing imaginal discs expressing Vps4DN.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Bx-MS1096-KE are reduced in size compared to controls.

      (Rodahl et al., 2009)

      Larvae expressing Vps4DN.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Cg.PA show a strong accumulation of autophagic structures in fat body cells (wild-type larval fat body cells show few autophagic structures). Some of these structures are autophagosomes, while others have a morphology consistent with material at various stages of degradation.

      (Rusten et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      NOT Enhancer of
      Statement
      Reference

      Vps4DN.UAS.Tag:HA, Scer\GAL4ppk.PU is a non-enhancer of endosome | P-stage phenotype of Rab5S43N.UAS, Scer\GAL4ppk.PU

      (Zhang et al., 2014)
      NOT Suppressor of
      Statement
      Reference

      Vps4DN.UAS.Tag:HA, Scer\GAL4ppk.PU is a non-suppressor of endosome | P-stage phenotype of Rab5S43N.UAS, Scer\GAL4ppk.PU

      (Zhang et al., 2014)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The disruption of the actin cytoskeleton that is seen in the wing imaginal discs of animals expressing Vps4DN.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Bx-MS1096-KE is partially suppressed by co-expression of either bskK53R.Scer\UAS or pucScer\UAS.cMa.

      Wing imaginal discs co-expressing both bskK53R.Scer\UAS and Vps4DN.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Bx-MS1096-KE overgrow and are larger in size compared to controls.

      Wing imaginal discs co-expressing both pucScer\UAS.cMa and Vps4DN.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Bx-MS1096-KE are larger in size compared to controls.

      (Rodahl et al., 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Vps4DN.Scer\UAS.T:Ivir\HA1
      Vps4DN.UAS.Tag:HA
      vps4DN
      (Rusten et al., 2007)
      Name Synonyms
      Secondary FlyBase IDs
        References (7)