FlyBase Allele Report: Dmel\Atg13[UAS.EGFP]

General Information

Symbol

Dmel\Atg13^UAS.EGFP

Species

D. melanogaster

Name

FlyBase ID

FBal0221505

Feature type

allele

Associated gene

Dmel\Atg13

Associated Insertion(s)

Carried in Construct

P{UAS-Atg13-GFP}

Also Known As

UAS-Atg13-GFP, UAS-Atg13

Key Links

Transgenic product class

wild_type

(Chang and Neufeld, 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Chang and Neufeld, 2009)

Mutagen

in vitro construct

(Chang and Neufeld, 2009)

Progenitor genotype

Carried in construct

P{UAS-Atg13-GFP}

Cytology

Description

UASt regulatory sequences drive expression of Atg13 which is tagged with EGFP.

(Chang and Neufeld, 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Atg13

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Clones of cells in the fat body expressing Atg13^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Act5C.PP fail to induce autophagy in response to starvation.

(Chang and Neufeld, 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible | adult stage phenotype, suppressible by Idit^JF02930, Scer\GAL4^GMR.PF

(Cobb et al., 2023)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF has increased cell death | adult stage phenotype, suppressible by Idit^JF02930, Scer\GAL4^GMR.PF

(Cobb et al., 2023)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has visible phenotype, suppressible | partially by Gyf^HM05106, Scer\GAL4^GMR.PU

(Kim et al., 2015)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has visible phenotype, suppressible by Atg1^JF02273, Scer\GAL4^GMR.PU

(Kim et al., 2015)

NOT suppressed by

Statement

Reference

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has visible phenotype, non-suppressible by eIF4EHP^GL01035, Scer\GAL4^GMR.PU

(Kim et al., 2015)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has visible phenotype, non-suppressible by pico^GD7437, Scer\GAL4^GMR.PU

(Kim et al., 2015)

Other

Statement

Reference

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible | adult stage phenotype

(Cobb et al., 2023)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF has increased cell death | adult stage phenotype

(Cobb et al., 2023)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has fertile phenotype

(Kim et al., 2015)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has increased cell death phenotype

(Kim et al., 2015)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has visible phenotype

(Kim et al., 2015)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has sterile phenotype

(Kim et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Idit^JF02930, Scer\GAL4^GMR.PF

(Cobb et al., 2023)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF has autolysosome | increased number | adult stage phenotype, suppressible by Idit^JF02930, Scer\GAL4^GMR.PF

(Cobb et al., 2023)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has eye phenotype, suppressible | partially by Gyf^HM05106, Scer\GAL4^GMR.PU

(Kim et al., 2015)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has eye phenotype, suppressible by Atg1^JF02273, Scer\GAL4^GMR.PU

(Kim et al., 2015)

NOT suppressed by

Statement

Reference

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has eye phenotype, non-suppressible by eIF4EHP^GL01035, Scer\GAL4^GMR.PU

(Kim et al., 2015)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has eye phenotype, non-suppressible by pico^GD7437, Scer\GAL4^GMR.PU

(Kim et al., 2015)

Other

Statement

Reference

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF has eye phenotype

(Cobb et al., 2023)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF has autolysosome | increased number | adult stage phenotype

(Cobb et al., 2023)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has eye phenotype

(Kim et al., 2015)

Atg13^UAS.EGFP, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PU has eye disc phenotype

(Kim et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Atg1^{Scer\UAS.T:Hsap\MYC} and Atg13^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PU at 18[o]C results in reduced viability and fertility, as well as eye degeneration. Raising the flies at 18[o]C restores fertility, whilst maintaining the eye degeneration. Retinal cells such as photoreceptors and cone cells exhibit degeneration and there is complete disarray of the ommatidia structure and accumulation of membrane-dense vacuoles. After 4 weeks the retinal structure has completely degenerated except for the corneal lens exocuticle. Prominent cell death is seen in the differentiation ommatidia in eye disc and autophagic flux is increased.

Expression of Gyf^HM05106 dramatically suppresses the eye degeneration and eye disc cell death seen when Atg1^{Scer\UAS.T:Hsap\MYC} and Atg13^{Scer\UAS.T:Avic\GFP-EGFP} are co-expressed under the control of Scer\GAL4^GMR.PU at 18[o]C. However, the photoreceptor morphology is not fully restored. The increased autophagic flux in eye discs is also suppressed.

Expression of Atg1^JF02273 completely suppresses the eye degeneration seen when Atg1^{Scer\UAS.T:Hsap\MYC} and Atg13^{Scer\UAS.T:Avic\GFP-EGFP} are co-expressed under the control of Scer\GAL4^GMR.PU at 18[o]C.

Expression of 4EHP^GL01035 completely does not suppress the eye degeneration seen when Atg1^{Scer\UAS.T:Hsap\MYC} and Atg13^{Scer\UAS.T:Avic\GFP-EGFP} are co-expressed under the control of Scer\GAL4^GMR.PU at 18[o]C.

Expression of pico^GD7437 does not suppress the eye degeneration seen when Atg1^{Scer\UAS.T:Hsap\MYC} and Atg13^{Scer\UAS.T:Avic\GFP-EGFP} are co-expressed under the control of Scer\GAL4^GMR.PU at 18[o]C.

(Kim et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Atg13^{Scer\UAS.T:Avic\GFP-EGFP}

Atg13^UAS.EGFP

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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