FlyBase Allele Report: Dmel\Blos1[EY06269]

General Information

Symbol

Dmel\Blos1^EY06269

Species

D. melanogaster

Name

FlyBase ID

FBal0222451

Feature type

allele

Associated gene

Dmel\Blos1

Associated Insertion(s)

P{EPgy2}Blos1^EY06269

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{EPgy2}Blos1^EY06269

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{EPgy2}

misexpression element

Regulatory region(s)

UASp

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:14,601,299..14,601,299 [-]

(Mullin et al., 2015, FlyBase Curators, 2008-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of schizophrenia

CEA

(Mullin et al., 2015)

CEA with Dysb^e01028

(Mullin et al., 2015)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal learning

(Mullin et al., 2015)

abnormal neuroanatomy | dominant

(Mullin et al., 2015)

abnormal neurophysiology | dominant

(Mullin et al., 2015)

Phenotype Manifest In

NMJ bouton | increased number

(Mullin et al., 2015)

Detailed Description

Statement

Reference

Neither heterozygous or homozygous Blos1^EY06269 has an effect on excitatory junction potential (EJP) amplitude or miniature EJP frequency compared to controls. However both homozygous and heterozygous Blos1^EY06269 mutant flies have increased mEJP amplitude and a correspondingly lower quantal content. Blos1^EY06269 mutants show a dominant increase in the number of boutons per muscle at the 6/7 synapse, but overall muscle area is unaffected. Homozygous Blos1^EY06269 mutant flies show the normal increase in quantal content observed in response to philanotoxin. The rate of vesicle depletion at low frequency stimulation is similar to controls, but rate of vesicle depletion is increased at high frequency stimulation.

Homozygous Blos1^EY06269 mutant flies exhibit deficits in short term olfactory habituation.

(Mullin et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Blos1^EY06269 has abnormal neurophysiology | dominant phenotype, suppressible by Dysb[+]/Dysb^e01028

(Mullin et al., 2015)

Blos1^EY06269 has abnormal neurophysiology | dominant phenotype, suppressible by Df(3L)BSC416/+

(Mullin et al., 2015)

Blos1^EY06269 has abnormal neuroanatomy | dominant phenotype, suppressible by Dysb[+]/Dysb^e01028

(Mullin et al., 2015)

NOT suppressed by

Statement

Reference

Blos1^EY06269 has abnormal neurophysiology | dominant phenotype, non-suppressible by Dysb^rv/Dysb[+]

(Mullin et al., 2015)

Blos1^EY06269 has abnormal neurophysiology | dominant phenotype, non-suppressible by Scer\GAL4^elav-C155/Dysb[+]/Dysb^UAS.cDa/Dysb^e01028

(Mullin et al., 2015)

Blos1^EY06269 has abnormal neuroanatomy | dominant phenotype, non-suppressible by Dysb^rv/Dysb[+]

(Mullin et al., 2015)

Other

Statement

Reference

Blos1^EY06269, Dysb[+]/Dysb^e01028 has abnormal neurophysiology phenotype

(Mullin et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Blos1^EY06269 has NMJ bouton | increased number phenotype, suppressible by Dysb[+]/Dysb^e01028

(Mullin et al., 2015)

NOT suppressed by

Statement

Reference

Blos1^EY06269 has NMJ bouton | increased number phenotype, non-suppressible by Dysb^rv/Dysb[+]

(Mullin et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Blos1^EY06269 Dysb^e01028 double heterozygotes have no effect on excitatory junction potential (EJP) amplitude or miniature EJP frequency compared to controls. Synaptic vesicles are similar in size to controls. The increase in quantal content normally observed in response to philanotoxin is not seen. One copy of Dysb^e01028 suppresses the dominant changes in mEJP amplitude and quantal content seen in Blos1^EY06269/+ mutant NMJs. The increase in bouton number seen in Blos1^EY06269/+ NMJs is also suppressed.

Df(3L)BSC416 (which deletes Dysb), Blos1^EY06269 double heterozygotes have no effect on excitatory junction potential (EJP) amplitude or miniature EJP frequency compared to controls.

Blos1^EY06269 Dysb^rv double heterozygotes have no effect on excitatory junction potential (EJP) amplitude or miniature EJP frequency compared to controls. One copy of Dysb^rv suppresses the dominant changes in mEJP amplitude and quantal content seen in Blos1^EY06269/+ mutant NMJs. The increase in bouton number seen in Blos1^EY06269/+ NMJs is not suppressed.

Expression of Dysb^Scer\UAS.cDa under the control of Scer\GAL4^elav-C155 in a Blos1^EY06269/+; Dysb^e01028/+ mutant background has no effect on excitatory junction potential (EJP) amplitude or miniature EJP frequency compared to controls. The mEJP amplitude is increased, similar to that seen in Blos1^EY06269/+ mutant flies.

One copy of Df(3L)BSC416 suppresses the dominant changes in mEJP amplitude and quantal content seen in Blos1^EY06269/+ mutant NMJs.

One copy of Df(3L)BSC416 suppresses the increase in bouton number seen in Blos1^EY06269/+ NMJs.

(Mullin et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Blos1^EY06269 is rescued by Scer\GAL4^elav-C155/Blos1^UAS.cCa

(Mullin et al., 2015)

Comments

Pre-synaptic expression of Blos1^Scer\UAS.cCa under the control of Scer\GAL4^elav-C155 rescues the changes in mEJP amplitude seen in Blos1^EY06269 mutant NMJs.

(Mullin et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

16707

y¹ w^67c23; P{EPgy2}Blos1^EY06269

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Blos1^EY06269

Blos1^EY

(Mullin et al., 2015)

CG30077^EY06269

blos1^EY06269

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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