FlyBase Allele Report: Dmel\Gabat[f01602]

General Information

Symbol

Dmel\Gabat^f01602

Species

D. melanogaster

Name

FlyBase ID

FBal0222673

Feature type

allele

Associated gene

Dmel\Gabat

Associated Insertion(s)

PBac{WH}Gabat^f01602

Carried in Construct

Also Known As

gabat^f

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

PBac{WH}Gabat^f01602

Cytology

Description

Allele components

Component

Use(s)

Inserted element

PBac{WH}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:19,998,279..19,998,356 [+]

(Chen et al., 2015, FlyBase Curators, 2008-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal sleep

(Chen et al., 2015)

abnormal starvation stress response | adult stage

(Maguire et al., 2015)

short lived | nutrition conditional

(Maguire et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

Gabat^PL00338/Gabat^PL00338 males show significant increases in both day and night time sleep, with a increase in the fraction of sleep spent in long (>150min) sleep episodes and a significant decrease in sleep bout number at night (as well as a significant increase in the number of bouts during the day) compared to controls. Mutant flies show a significant decrease in sleep latency compared to controls. Activity per waking minute is similar to controls in mutants. Mutants generally show normal circadian rhythms, compared to wild type.

(Chen et al., 2015)

Gabat^f01602/Gabat^f01602 mutant adult males display severely reduced survival as compared to wild-type when reared on a sucrose only diet, but similar survival rates to wild type when reared on regular food.

(Maguire et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Gabat^f01602/Gabat^f01602 is a suppressor of abnormal sleep phenotype of qvr^EY04063

(Chen et al., 2015)

Gabat^f01602/Gabat^f01602 is a suppressor of abnormal locomotor rhythm phenotype of qvr^EY04063

(Chen et al., 2015)

Other

Statement

Reference

DAT^fmn, Gabat^f01602 has abnormal sleep phenotype

(Chen et al., 2015)

Gabat^{UAS.Tag:V5,Tag:polyHis}, Gabat^f01602, Scer\GAL4^{Gabat-PL00338}, qvr^EY04063 has abnormal sleep phenotype

(Chen et al., 2015)

Gabat^{UAS.Tag:V5,Tag:polyHis}, Gabat^f01602, Scer\GAL4^repo.PU, qvr^EY04063 has abnormal sleep phenotype

(Chen et al., 2015)

Gabat^{UAS.Tag:V5,Tag:polyHis}, Gabat^f01602, Scer\GAL4^Gad1.3.098, qvr^EY04063 has abnormal sleep phenotype

(Chen et al., 2015)

Alat^EY06928, Gabat^f01602 has lethal phenotype

(Maguire et al., 2015)

Gabat^f01602, Got1^DG14503 has abnormal starvation stress response | adult stage phenotype

(Maguire et al., 2015)

Gabat^f01602, Got1^DG14503 has short lived | nutrition conditional phenotype

(Maguire et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Gabat^f01602/Gabat^f01602 partially suppresses sleep phenotypes seen in qvr^EY04063/qvr^EY04063 flies; in addition, double mutants show an increase in the fraction of long sleep episodes and reductions in nighttime sleep bout numbers (similar to Gabat^f01602/Gabat^f01602 single mutants).

Expression of Gabat^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} driven by Scer\GAL4^{Gabat-PL00338} or Scer\GAL4^repo.PU (but not Scer\GAL4^Gad1.3.098) in a qvr^EY04063/qvr^EY04063;Gabat^f01602/Gabat^f01602 double mutant background diminishes sleep to around 35% (sleep is also more fragmented and sleep latency increases, whereas sleep bout number is unchanged) of qvr^EY04063/qvr^EY04063;Gabat^f01602/Gabat^f01602 (partial rescue).

(Chen et al., 2015)

Got1^DG14503/Got1^DG14503, Gabat^f01602/Gabat^f01602 double mutants display severely reduced survival in response to a sucrose only diet.

CG1640^EY06928/CG1640^EY06928, Gabat^f01602/Gabat^f01602 double mutants are lethal.

(Maguire et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Gabat^f01602 is rescued by Scer\GAL4^elav.PU/Gabat^{UAS.Tag:V5,Tag:polyHis}

(Maguire et al., 2015)

Gabat^f01602 is rescued by Scer\GAL4^repo.PU/Gabat^{UAS.Tag:V5,Tag:polyHis}

(Maguire et al., 2015)

Comments

Expression of Gabat^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of Scer\GAL4^elav.PU or Scer\GAL4^repo.PU rescues the reduced survival of Gabat^f01602/Gabat^f01602 mutants raised on a sucrose only diet.

(Maguire et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

85192

w¹¹¹⁸; PBac{WH}Gabat^f01602/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CG7433^f01602

Gabat^f01602

gabat^F

(Chen et al., 2015)

gabat^f01602

(Maguire et al., 2015)

gabat^f

(Maguire et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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