FlyBase Allele Report: Dmel\Max[RNAi.UAS]

General Information

Symbol

Dmel\Max^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0230162

Feature type

allele

Associated gene

Dmel\Max

Associated Insertion(s)

Carried in Construct

P{UAS-Max.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Steiger et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Steiger et al., 2008)

Mutagen

in vitro construct

(Steiger et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-Max.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of a direct inverted repeat of a 784bp Max cDNA fragment (corresponding to nucleotides 312-915 of Max-RA).

(Steiger et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Max

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased body size, with Scer\GAL4^Act5C.PI

(Steiger et al., 2008)

lethal - all die before end of pupal stage, with Df(3L)fz2, Scer\GAL4^Act5C.PI

(Steiger et al., 2008)

lethal - all die before end of pupal stage, with Max¹, Scer\GAL4^Act5C.PI

(Steiger et al., 2008)

lethal - all die before end of pupal stage, with Scer\GAL4^Act5C.PI

(Steiger et al., 2008)

lethal - all die before end of pupal stage (with Max¹), with Scer\GAL4^Act5C.PI

(Steiger et al., 2008)

some die during pupal stage, with Df(3L)fz2, Scer\GAL4^Act5C.PI

(Steiger et al., 2008)

some die during pupal stage, with Max¹, Scer\GAL4^Act5C.PI

(Steiger et al., 2008)

some die during pupal stage, with Scer\GAL4^Act5C.PI

(Steiger et al., 2008)

some die during pupal stage (with Max¹), with Scer\GAL4^Act5C.PI

(Steiger et al., 2008)

visible, with Scer\GAL4^ap-md544

(Steiger et al., 2008)

visible, with Scer\GAL4^dpp.blk1

(Steiger et al., 2008)

visible, with Scer\GAL4^sca-109-68

(Steiger et al., 2008)

Phenotype Manifest In

anterior scutellar bristle, with Scer\GAL4^dpp.blk1

(Steiger et al., 2008)

posterior scutellar bristle, with Scer\GAL4^dpp.blk1

(Steiger et al., 2008)

scutellar bristle, with Scer\GAL4^sca-109-68

(Steiger et al., 2008)

wing, with Scer\GAL4^ap-md544

(Steiger et al., 2008)

Detailed Description

Statement

Reference

Expression of Max^{dsRNA.Scer\UAS.IR} under the control of either Scer\GAL4^dpp.blk1 or Scer\GAL4^sca-109-68 results in a decrease in size of the scutellar bristles compared to controls.

Expression of Max^{dsRNA.Scer\UAS.IR} under the control of Scer\GAL4^ap-md544 results in bending up of the wings.

Males and females expressing Max^{dsRNA.Scer\UAS.IR} under the control of Scer\GAL4^Act5C.PI have a reduced average weight compared to control flies.

The survival rate and growth of Max¹ larvae is not reduced if the animals are also expressing Max^{dsRNA.Scer\UAS.IR} under the control of Scer\GAL4^Act5C.PI.

Expression of one copy of Max^{dsRNA.Scer\UAS.IR} under the control of Scer\GAL4^Act5C.PI has only a marginal effect on viability. Expression of three copies of Max^{dsRNA.Scer\UAS.IR} under the control of Scer\GAL4^Act5C.PI results in complete lethality at the pupal stage. Expression of one copy of Max^{dsRNA.Scer\UAS.IR} under the control of Scer\GAL4^Act5C.PI results in complete lethality at the pupal stage in the presence of Df(3L)fz2 or Max¹.

(Steiger et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Max^RNAi.UAS, Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Myc^UAS.cZa, Scer\GAL4^GMR.PF

(Steiger et al., 2008)

Other

Statement

Reference

Max^RNAi.UAS, Myc^P0, Scer\GAL4^da.G32 has lethal phenotype

(Steiger et al., 2008)

Phenotype Manifest In

Suppressor of

Statement

Reference

Max^RNAi.UAS, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Myc^UAS.cZa, Scer\GAL4^GMR.PF

(Steiger et al., 2008, Steiger et al., 2008)

NOT Suppressor of

Statement

Reference

Max^RNAi.UAS, Scer\GAL4^GMR.PF is a non-suppressor of pigment cell phenotype of Myc^UAS.cZa, Scer\GAL4^GMR.PF

(Steiger et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

dm^P0/Y ; flies expressing Max^{dsRNA.Scer\UAS.IR} under the control of Scer\GAL4^da.G32 show a strong reduction in viability compared to controls.

Co-expression of Max^{dsRNA.Scer\UAS.IR} does not suppress the eye apoptosis phenotype caused by expression of three copies of dm^Scer\UAS.cZa under the control of Scer\GAL4^GMR.PF. The increase in ommatidial size caused by expression of dm^Scer\UAS.cZa under the control of Scer\GAL4^GMR.PF is suppressed by co-expression of Max^{dsRNA.Scer\UAS.IR}.

(Steiger et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Max^RNAi.UAS.IR

Max^RNAi.UAS

Max^{dsRNA.Scer\UAS.IR}

Max^dsRNA.UAS.IR

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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