FlyBase Allele Report: Dmel\crol[UAS.cMa]

General Information

Symbol

Dmel\crol^UAS.cMa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Mitchell

FlyBase ID

FBal0230468

Feature type

allele

Associated gene

Dmel\crol

Associated Insertion(s)

Carried in Construct

P{UAS-crol.M}

Key Links

Transgenic product class

cDNA

(Mitchell et al., 2008)

wild_type

(Mitchell et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Mitchell et al., 2008)

wild_type

(Mitchell et al., 2008)

Mutagen

in vitro construct

(Mitchell et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-crol.M}

Cytology

Description

UAS regulatory fusion drives expression of a full length crol cDNA, which encodes a 962 amino acid residue protein.

(Mitchell et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

crol

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death, with Scer\GAL4^en-e16E

(Mitchell et al., 2008)

increased occurrence of cell division | larval stage, with Scer\GAL4^en-e16E

(Mitchell et al., 2008)

visible, with Scer\GAL4^en-e16E

(Mitchell et al., 2008)

Phenotype Manifest In

wing blade posterior compartment, with Scer\GAL4^en-e16E

(Mitchell et al., 2008)

wing disc, with Scer\GAL4^en-e16E

(Mitchell et al., 2008)

wing pouch, with Scer\GAL4^en-e16E

(Mitchell et al., 2008)

Detailed Description

Statement

Reference

Increased BrdU incorporation is seen in the posterior compartment of the wing pouch zone of non-proliferating cells (ZNC) of wing discs expressing crol^Scer\UAS.cMa under the control of Scer\GAL4^en-e16E. The posterior compartment ZNC contains mitotic cells in these discs.

Wing disc posterior compartment cells expressing crol^Scer\UAS.cMa under the control of Scer\GAL4^en-e16E show massive levels of apoptosis.

The posterior compartment of the wing is smaller than that of controls in adults expressing crol^Scer\UAS.cMa under the control of Scer\GAL4^en-e16E.

(Mitchell et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^en-e16E, crol^UAS.cMa has visible phenotype, enhanceable by arm⁴/arm[+]

(Mitchell et al., 2008)

Scer\GAL4^en-e16E, crol^UAS.cMa has increased occurrence of cell division | larval stage phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^en-e16E

(Mitchell et al., 2008)

Suppressed by

Statement

Reference

Scer\GAL4^en-e16E, crol^UAS.cMa has visible phenotype, suppressible by dm[+]/Myc^P0

(Mitchell et al., 2008)

Scer\GAL4^en-e16E, crol^UAS.cMa has visible phenotype, suppressible by CycE^AR95/CycE[+]

(Mitchell et al., 2008)

Scer\GAL4^en-e16E, crol^UAS.cMa has visible phenotype, suppressible by stg^AR2/stg[+]

(Mitchell et al., 2008)

Scer\GAL4^en-e16E, crol^UAS.cMa has visible phenotype, suppressible by CycB[+]/CycB²

(Mitchell et al., 2008)

Scer\GAL4^en-e16E, crol^UAS.cMa has visible phenotype, suppressible by E2f[+]/E2f1⁹¹

(Mitchell et al., 2008)

Scer\GAL4^en-e16E, crol^UAS.cMa has visible phenotype, suppressible by Axn^E77/Axn[+]

(Mitchell et al., 2008)

Scer\GAL4^en-e16E, crol^UAS.cMa has visible phenotype, suppressible by sgg[+]/sgg¹

(Mitchell et al., 2008)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^Act5C.PP, crol^UAS.cMa has increased occurrence of cell division | somatic clone | larval stage phenotype

(Mitchell et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^en-e16E, crol^UAS.cMa has wing blade posterior compartment phenotype, enhanceable by arm⁴/arm[+]

(Mitchell et al., 2008)

Suppressed by

Statement

Reference

Scer\GAL4^en-e16E, crol^UAS.cMa has wing blade posterior compartment phenotype, suppressible by E2f1[+]/E2f1⁹¹

(Mitchell et al., 2008)

Scer\GAL4^en-e16E, crol^UAS.cMa has wing blade posterior compartment phenotype, suppressible by Myc^P0/Myc[+]

(Mitchell et al., 2008)

Scer\GAL4^en-e16E, crol^UAS.cMa has wing blade posterior compartment phenotype, suppressible by Axn^E77/Axn[+]

(Mitchell et al., 2008)

Scer\GAL4^en-e16E, crol^UAS.cMa has wing blade posterior compartment phenotype, suppressible by CycB[+]/CycB²

(Mitchell et al., 2008)

Scer\GAL4^en-e16E, crol^UAS.cMa has wing blade posterior compartment phenotype, suppressible by stg^AR2/stg[+]

(Mitchell et al., 2008)

Scer\GAL4^en-e16E, crol^UAS.cMa has wing blade posterior compartment phenotype, suppressible by sgg[+]/sgg¹

(Mitchell et al., 2008)

Scer\GAL4^en-e16E, crol^UAS.cMa has wing blade posterior compartment phenotype, suppressible by CycE^AR95/CycE[+]

(Mitchell et al., 2008)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, crol^UAS.cMa has wing disc posterior compartment phenotype

(Mitchell et al., 2008)

BacA\p35^UAS.cHa, Scer\GAL4^Act5C.PP, crol^UAS.cMa has wing disc | somatic clone phenotype

(Mitchell et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

The reduced size of the posterior compartment of the wing that is seen in animals expressing crol^Scer\UAS.cMa under the control of Scer\GAL4^en-e16E is suppressed if they are also heterozygous for one of dm^P0, CycE^AR95, stg^AR2, CycB², E2f⁹¹, Axn^E77 or sgg¹.

The reduced size of the posterior compartment of the wing that is seen in animals expressing crol^Scer\UAS.cMa under the control of Scer\GAL4^en-e16E is enhanced is they are also heterozygous for arm⁴.

(Mitchell et al., 2008)

Xenogenetic Interactions

Statement

Reference

Wing discs co-expressing both BacA\p35^Scer\UAS.cHa and crol^Scer\UAS.cMa under the control of Scer\GAL4^en-e16E show a substantial increase in the size of the posterior compartment in 120 hour larvae. This increase in size is associated with a massive increase in S phase and mitosis.

Clones in the wing disc that are co-expressing both BacA\p35^Scer\UAS.cHa and crol^Scer\UAS.cMa under the control of Scer\GAL4^Act5C.PP are overgrown and show an increase in BrdU incorporation and in the number of mitotic cells, compared to clones expressing BacA\p35^Scer\UAS.cHa alone under the control of Scer\GAL4^Act5C.PP. Increased proliferation is also seen in cells adjacent to the clones.

(Mitchell et al., 2008)

Complementation and Rescue Data

Rescues

Scer\GAL4^Tub.PU/crol^UAS.cMa rescues crol^k05205

(Mitchell et al., 2008)

Comments

Expression of crol^Scer\UAS.cMa under the control of Scer\GAL4^tub in crol^k05205 homozygous clones results in rescue of clonal size and survival.

(Mitchell et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (2)