FlyBase Allele Report: Dmel\cic[KK100838]

General Information

Symbol

Dmel\cic^KK100838

Species

D. melanogaster

Name

FlyBase ID

FBal0230946

Feature type

allele

Associated gene

Dmel\cic

Associated Insertion(s)

Carried in Construct

P{KK100838}

Also Known As

UAS-cic-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK100838}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK100838

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased occurrence of cell division | adult stage, with Scer\GAL4^Delta.PU

(Jin et al., 2015)

increased occurrence of cell division | adult stage, with Scer\GAL4^NP5130

(Jin et al., 2015)

Phenotype Manifest In

adult midgut, with Scer\GAL4^Delta.PU

(Jin et al., 2015)

adult midgut, with Scer\GAL4^NP5130

(Jin et al., 2015)

adult midgut, with Scer\GAL80^Su(H).GBE

(Jin et al., 2015)

adult midgut | somatic clone, with Scer\GAL4^NP5130

(Jin et al., 2015)

intestinal stem cell | adult stage, with Scer\GAL4^Delta.PU

(Jin et al., 2015)

intestinal stem cell | adult stage, with Scer\GAL4^NP5130

(Jin et al., 2015)

intestinal stem cell | adult stage, with Scer\GAL80^Su(H).GBE

(Jin et al., 2015)

intestinal stem cell | adult stage | somatic clone, with Scer\GAL4^NP5130

(Jin et al., 2015)

Detailed Description

Statement

Reference

Expression of cic^KK100838 RNAi under the control of either Scer\GAL4^esg-NP5130 or Scer\GAL4^Dl.PU (using tub-Gal80[ts] to limit the time of RNAi expression) results in a dramatic overproliferation of intestinal stem cells (ISC) in the adult midgut and increase in ISC mitoses. Expression under the control of Scer\GAL80^Su(H).GBE also results in an increase in the number of mitotic cells though majority of these are outside of the Scer\GAL80^Su(H).GBE driver expression domain and likely represent a non-autonomous effect of cic^KK100838 RNAi on the proliferation of ISCs.

Expression of cic^KK100838 under the control of Scer\GAL4^esg-NP5130 in intestinal stem cell (ISC) somatic flip-out clones (using tub-Gal80[ts] to limit the time of expression/clone induction) in the adult midgut results in clone overgrowth and highly increased ISC division.

(Jin et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^NP5130, cic^KK100838 has increased occurrence of cell division | somatic clone | adult stage phenotype, suppressible by Scer\GAL4^NP5130, pnt^RNAi.UAS.cUa

(Jin et al., 2015)

NOT suppressed by

Statement

Reference

Scer\GAL4^NP5130, cic^KK100838 has increased occurrence of cell division | somatic clone | adult stage phenotype, non-suppressible by Egfr^RNAi.UAS.cUa, Scer\GAL4^NP5130

(Jin et al., 2015)

Scer\GAL4^NP5130, cic^KK100838 has increased occurrence of cell division | somatic clone | adult stage phenotype, non-suppressible by Ras85D^RNAi.UAS.cUa/Scer\GAL4^NP5130

(Jin et al., 2015)

Other

Statement

Reference

Scer\GAL4^Tub.PU, cic^KK100838, pnt^Δ88 has decreased cell number | somatic clone phenotype

(Jin et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^NP5130, cic^KK100838 has adult midgut phenotype, suppressible by Scer\GAL4^NP5130, pnt^RNAi.UAS.cUa

(Jin et al., 2015)

Scer\GAL4^NP5130, cic^KK100838 has intestinal stem cell | adult stage phenotype, suppressible by Scer\GAL4^NP5130, pnt^RNAi.UAS.cUa

(Jin et al., 2015)

NOT suppressed by

Statement

Reference

Scer\GAL4^NP5130, cic^KK100838 has adult midgut | somatic clone phenotype, non-suppressible by Egfr^RNAi.UAS.cUa, Scer\GAL4^NP5130

(Jin et al., 2015)

Scer\GAL4^NP5130, cic^KK100838 has intestinal stem cell | somatic clone | adult stage phenotype, non-suppressible by Egfr^RNAi.UAS.cUa, Scer\GAL4^NP5130

(Jin et al., 2015)

Scer\GAL4^NP5130, cic^KK100838 has adult midgut | somatic clone phenotype, non-suppressible by Ras85D^RNAi.UAS.cUa/Scer\GAL4^NP5130

(Jin et al., 2015)

Scer\GAL4^NP5130, cic^KK100838 has intestinal stem cell | somatic clone | adult stage phenotype, non-suppressible by Ras85D^RNAi.UAS.cUa/Scer\GAL4^NP5130

(Jin et al., 2015)

Other

Statement

Reference

Scer\GAL4^Tub.PU, cic^KK100838, pnt^Δ88 has adult midgut | somatic clone phenotype

(Jin et al., 2015)

Scer\GAL4^Tub.PU, cic^KK100838, pnt^Δ88 has intestinal stem cell | somatic clone | adult stage phenotype

(Jin et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The overgrowth and increased mitotic rate of intestinal stem cell somatic flip-out clones expressing cic^KK100838 under the control of Scer\GAL4^esg-NP5130 (using tub-Gal80[ts] to limit the time of expression/clone induction) in the adult midgut cannot be suppressed by co-expression of either Egfr^{dsRNA.Scer\UAS.cUa} or Ras85D^{dsRNA.Scer\UAS.cUa}.

The overproliferation of intestinal stem cells and the increase in the mitotic rate in the adult midgut observed in flies expressing cic^KK100838 under the control of either Scer\GAL4^esg-NP5130 or Scer\GAL4^Dl.PU (using tub-Gal80[ts] to limit the time of RNAi expression) is strongly suppressed by co-expression of pnt^{dsRNA.Scer\UAS.cUa}.

The growth of intestinal stem cell somatic MARCM clones expressing cic^KK100838 under the control of Scer\GAL4^tub.PU in the adult midgut is significantly impaired by combination with pnt^Δ88 in homozygous state, while combination with either pnt^05484Δ33 or pnt^07825Δ78 does not reduce the clone growth.

(Jin et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v103805

P{KK100838}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

cic^KK100838

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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