FlyBase Allele Report: Dmel\N[KK102890]

General Information

Symbol

Dmel\N^KK102890

Species

D. melanogaster

Name

FlyBase ID

FBal0232074

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

P{KK102890}

Also Known As

UAS-N-RNAi, UAS-Notch^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK102890}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK102890

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal dorsal adult muscle precursor cell | third instar larval stage, with Scer\GAL4^{E(spl)m6-BFM.PF}

(Aradhya et al., 2015)

abdominal lateral adult muscle precursor cell | third instar larval stage, with Scer\GAL4^{E(spl)m6-BFM.PF}

(Aradhya et al., 2015)

adult anterior midgut epithelium, with Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Zhai et al., 2015)

adult posterior midgut epithelium, with Scer\GAL4^NP6267

(Okumura et al., 2016)

adult posterior midgut epithelium | female limited, with Scer\GAL4^NP7397

(Hudry et al., 2016)

apterous-expressing neuron of the lateral cluster of the thorax, with Dcr-2^UAS.cDa, N^RNAi.P.UAS, Scer\GAL4^elav-C155

(Ulvklo et al., 2012)

embryonic/larval brain | third instar larval stage, with Scer\GAL4^C855a

(Morante et al., 2013)

enterocyte of posterior adult midgut epithelium, with Scer\GAL4^NP6267

(Okumura et al., 2016)

female germline cyst, with Scer\GAL4^NP1624

(Berns et al., 2014)

follicle cell, with Scer\GAL4^NP1624

(Berns et al., 2014)

stalk follicle cell, with Scer\GAL4^NP1624

(Berns et al., 2014)

Detailed Description

Statement

Reference

Under control conditions, the adulthood-only expression of N^KK102890 under the control of Scer\GAL4^esg-NP7397 (and Gal80[ts], for the temporal regulation of expression) leads to a significant increase in the adult posterior midgut mitotic index in females only, as compared to controls.

(Hudry et al., 2016)

Expression of N^KK102890 RNAi under the control of Scer\GAL4^NP6267 (using tubGal80[ts] to limit the time of expression) results in enterocyte differentiation defects in the adult posterior midgut.

(Okumura et al., 2016)

The expression of N^KK102890 under the control of Scer\GAL4^{E(spl)m6-BFM.PF} leads to significantly decreased numbers of abdominal lateral and dorsal, but not ventral, adult muscle precursor cells in mid-third instar larvae, as compared to controls.

(Aradhya et al., 2015)

Expression of N^KK102890 under the control of Scer\GAL4^esg-NP5130 (limited to the adult stages using Scer\GAL80^ts.αTub84B) results in tumors in the anterior midgut.

(Zhai et al., 2015)

Scer\GAL4^tj-NP1624-mediated expression results in the absence of stalks in germaria and a decrease in follicle cell division in the germarium.

(Berns et al., 2014)

Scer\GAL4^C855a-mediated expression of N^KK102890 results in the wave of neuroepithelial-neuroblast transition moving faster across the larval brain - ectopic neuroblasts and overgrown neuroepithelium is not seen.

(Morante et al., 2013)

Embryos co-expressing N^KK102890 and N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^elav-C155 (in the presence of Dcr-2^Scer\UAS.cDa to enhance RNAi) have an increased number of "Ap" neurons in the NB5-6T lineage.

(Ulvklo et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

N^KK102890, Scer\GAL4^NP7397 has increased occurrence of cell division | female limited | adult stage phenotype, suppressible by tra^JF03132, Scer\GAL4^NP7397

(Hudry et al., 2016)

N^KK102890, Scer\GAL4^NP7397 has increased occurrence of cell division | female limited | adult stage phenotype, suppressible by tra^KO.Cherry/Df(3L)st-j7

(Hudry et al., 2016)

N^KK102890, Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B has neoplasia phenotype, suppressible by upd2^NIG.5988R, Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Zhai et al., 2015)

NOT suppressed by

Statement

Reference

N^KK102890, Scer\GAL4^NP7397 has increased occurrence of cell division | female limited | adult stage phenotype, non-suppressible by dsx^Δ/fru^P1.LexA/dsx¹/Df(3R)Exel6179

(Hudry et al., 2016)

Suppressor of

Statement

Reference

Scer\GAL4^Tub.PU/N^KK102890 is a suppressor | somatic clone of abnormal neuroanatomy | somatic clone | larval stage phenotype of nerfin-1¹⁵⁹

(Xu et al., 2017)

Scer\GAL4^Tub.PU/N^KK102890 is a suppressor | somatic clone of neoplasia | somatic clone | adult stage phenotype of nerfin-1¹⁵⁹

(Xu et al., 2017)

N^KK102890, Scer\GAL4^{E(spl)m6-BFM.PF} is a suppressor of increased cell number | third instar larval stage phenotype of InR^K414P.UAS, Scer\GAL4^{E(spl)m6-BFM.PF}

(Aradhya et al., 2015)

N^KK102890, Scer\GAL4^NP1624 is a suppressor of increased cell number phenotype of Scer\GAL4^NP1624, Vps60^KK109120

(Berns et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

N^KK102890, Scer\GAL4^NP7397 has adult posterior midgut epithelium | female limited phenotype, suppressible by tra^JF03132, Scer\GAL4^NP7397

(Hudry et al., 2016)

N^KK102890, Scer\GAL4^NP7397 has adult posterior midgut epithelium | female limited phenotype, suppressible by tra^KO.Cherry/Df(3L)st-j7

(Hudry et al., 2016)

N^KK102890, Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B has adult anterior midgut epithelium phenotype, suppressible by upd2^NIG.5988R, Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Zhai et al., 2015)

NOT suppressed by

Statement

Reference

N^KK102890, Scer\GAL4^NP7397 has adult posterior midgut epithelium | female limited phenotype, non-suppressible by dsx^Δ/fru^P1.LexA/dsx¹/Df(3R)Exel6179

(Hudry et al., 2016)

N^KK102890, Scer\GAL4^NP6267 has adult posterior midgut epithelium phenotype, non-suppressible by GATAe^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^NP6267

(Okumura et al., 2016)

N^KK102890, Scer\GAL4^NP6267 has enterocyte of posterior adult midgut epithelium phenotype, non-suppressible by GATAe^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^NP6267

(Okumura et al., 2016)

Suppressor of

Statement

Reference

Scer\GAL4^Tub.PU/N^KK102890 is a suppressor | somatic clone of neuroblast | ectopic | somatic clone | larval stage phenotype of nerfin-1¹⁵⁹

(Xu et al., 2017)

Scer\GAL4^Tub.PU/N^KK102890 is a suppressor | somatic clone of adult optic lobe | somatic clone | larval stage phenotype of nerfin-1¹⁵⁹

(Xu et al., 2017)

N^KK102890, Scer\GAL4^{E(spl)m6-BFM.PF} is a suppressor of abdominal lateral adult muscle precursor cell | third instar larval stage phenotype of InR^K414P.UAS, Scer\GAL4^{E(spl)m6-BFM.PF}

(Aradhya et al., 2015)

N^KK102890, Scer\GAL4^{E(spl)m6-BFM.PF} is a suppressor of abdominal dorsal adult muscle precursor cell | third instar larval stage phenotype of InR^K414P.UAS, Scer\GAL4^{E(spl)m6-BFM.PF}

(Aradhya et al., 2015)

Scer\GAL4^NP1624/N^KK102890 is a suppressor of epithelium | somatic clone phenotype of Vps60^{d08528-f02548}

(Berns et al., 2014)

Scer\GAL4^NP1624/N^KK102890 is a suppressor of stalk follicle cell | somatic clone phenotype of Vps60^{d08528-f02548}

(Berns et al., 2014)

Scer\GAL4^NP1624/N^KK102890 is a suppressor of female germline cyst | somatic clone phenotype of Vps60^{d08528-f02548}

(Berns et al., 2014)

N^KK102890, Scer\GAL4^NP1624 is a suppressor of epithelium phenotype of Scer\GAL4^NP1624, Vps60^KK109120

(Berns et al., 2014)

N^KK102890, Scer\GAL4^NP1624 is a suppressor of stalk follicle cell phenotype of Scer\GAL4^NP1624, Vps60^KK109120

(Berns et al., 2014)

N^KK102890, Scer\GAL4^NP1624 is a suppressor of female germline cyst phenotype of Scer\GAL4^NP1624, Vps60^KK109120

(Berns et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Clonal expression of N^KK102890 under the control of Scer\GAL4^tub.PU largely suppresses the formation of ectopic neuroblasts in larval optic lobe nerfin-1¹⁵⁹/nerfin-1¹⁵⁹ mutant clones, and suppresses tumor formation at the adult stage in these clones.

(Xu et al., 2017)

The increased mitotic index in the female adult posterior midgut induced by the adulthood-only expression of N^KK102890 under the control of Scer\GAL4^esg-NP7397 (and Gal80[ts], for the temporal regulation of expression) is suppressed by the co-expression of tra^JF03132 or by tra^KO.Cherry/Df(3L)st-j7 transheterozygosity, but not by fru^P1.LexA/Df(3R)Exel6179, dsx¹/dsx^Δ double transheterozygosity.

(Hudry et al., 2016)

The enterocyte differentiation defects observed in adult posterior midgut expressing N^KK102890 RNAi under the control of Scer\GAL4^NP6267 (using tubGal80[ts] to limit the time of expression) is not rescued by co-expression of GATAe^{Scer\UAS.T:SV5\V5,T:Zzzz\His6}.

(Okumura et al., 2016)

The increased number of abdominal lateral and dorsal adult muscle precursor cells present in third instar larvae expressing InR^{R418P.Scer\UAS} under the control of Scer\GAL4^{E(spl)m6-BFM.PF} is suppressed by co-expressing N^KK102890, but no significant effect on the number of abdominal ventral adult muscle precursor cells is seen in these larvae, as compared to controls.

(Aradhya et al., 2015)

Expression of upd2^HMS00901 reduces tumor formation in the anterior midgut when N^KK102890 is expressed under the control of Scer\GAL4^esg-NP5130 (limited to the adult stages using Scer\GAL80^ts.αTub84B).

(Zhai et al., 2015)

Co-expression of N^KK102890 prevents the formation of elongated stalks, the disorganization of germaria, and the increased cell numbers seen in Vps60^KK109120 Scer\GAL4^tj-NP1624 mutants.

Scer\GAL4^tj-NP1624-mediated expression of N^KK102890 prevents the formation of elongated stalks and the disorganization of germaria seen in germaria are composed mostly of Vps60^{d08528-f02548} cells.

(Berns et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v100002

P{KK102890}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

N^KK102890

Name Synonyms

Secondary FlyBase IDs

References (19)

Report Sections

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