UASt regulatory sequences drive expression of an inverted repeat.
Expression of Sam-SKK107709 under the control of Scer\GAL4esg-NP5130 together for tubGal80[ts] to bypass the developmental stage or with also the Scer\GAL80Su(H).GBE added for intestinal stem cell-specific knock-down, results in significantly reduced number of proliferating (EdU+) cells in the adult midgut. The Scer\GAL4esg-NP5130-driven knock-down of Sam-S also prevents the increase in cell division observed upon refeeding and results in reduced number of enteroblasts (but not intestinal stem cells) in the posterior midgut epithelium.
Expression of Sam-SKK107709 under Scer\GAL4GMR11F02 leads to a marked reduction in the number of mitotic (pH3+) cells in the third instar larval wing disc and results in practically complete wing loss (only severely deformed little stumps remain) in adult flies. Scer\GAL4nos.PG-controlled expression (with UAS-Dicer2 to enhance RNAi efficacy) induces loss of germline stem cells in female germaria, as compared to controls.
Individuals expressing Sam-SKK107709 under the control of Scer\GAL4fat or under the combined control of Scer\GAL4αTub84B.Switch.PK and RU486 treatment are short lived compared to controls.
SamsKK107709, Scer\GAL4NP5130 is a suppressor of increased occurrence of cell division | adult stage phenotype of InRA1325D.UAS, Scer\GAL4NP5130
SamsKK107709, Scer\GAL4NP5130 is a suppressor of adult midgut phenotype of InRA1325D.UAS, Scer\GAL4NP5130
The increase in the number of proliferating (EdU+) cells observed in the posterior midgut of adult flies upon expression of InRA1325D.Scer\UAS driven by Scer\GAL4esg-NP5130 (combined with Gal80[ts] for temporal control) is completely abolished by co-expression of Sam-SKK107709.
