FB2024_02 , released April 23, 2024
Allele: Dmel\jpKK107921
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General Information
Symbol
Dmel\jpKK107921
Species
D. melanogaster
Name
FlyBase ID
FBal0235256
Feature type
allele
Associated gene
Dmel\jp
Associated Insertion(s)
Carried in Construct
P{KK107921}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
in vitro construct - RNAi
(Keleman et al., 2009.8.5)
in vitro construct - regulatory fusion
(Keleman et al., 2009.8.5)
Progenitor genotype
Carried in construct
P{KK107921}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-KK107921
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  cardiomyopathy
      CEA
      (Calpena et al., 2018)
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of jpKK107921 under the control of either Scer\GAL4Act5C.PU or Scer\GAL4twi.PU combined with Scer\GAL4Mef2.PU leads to early lethality, with the Scer\GAL4Mhc.PU driver viable adults do emerge and even have slightly extended lifespan but they also show rapid age-progressive decline in either climbing or flight ability compared to controls, and although their indirect flight muscles do not display any obvious morphological defects in week-old flies, the muscle area is significantly increased in 4-week-old adults and mitochondria therein are smaller and more rounded that in muscles from controls. The diads in the muscles are only rudimentary or have aberrant morphology including a vacuolated sarcomeric reticulum and the adjoining mitochondrial regions also have abnormal cristae morphology.

      Scer\GAL4tin.cBa-driven expression of jpKK107921 results in reduced adult lifespan and median survival at 29[o]C compared to controls, and whereas the diastolic interval, systolic interval or heart period length and arrhythmia index in 1-week-old flies at 29[o]C are all comparable to controls, the end-systolic and end-diastolic are significantly increased and the fractional shortening percentage is decreased; the cardiac chambers are enlarged but the thickness of the heart wall is not significantly different from controls. The parallel alignment of transverse myofibrils in the heart tube in the areas surrounding the ostia in jpKK107921-expressing flies is disturbed.

      Adult flies expressing jpKK107921 under the Scer\GAL4elav.PU driver have reduced survival rate and display significantly longer recovery times in bang-sensitive analyses at both 1 and 4 weeks after eclosion. Scer\GAL4GMR.PU-driven expression has no obvious morphological phenotype in the adult eye and does not induce depigmentation even in aged flies, but the ommatidia frequently contain fewer photoreceptor cells.

      Scer\GAL4rn-GAL4- or Scer\GAL4nub.PU-driven expression of jpKK107921 has no overt effect on adult wing morphology.

      (Calpena et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhancer of
      Suppressor of
      Statement
      Reference

      Scer\GAL4nub.PU/jpKK107921 is a suppressor of wing vein phenotype of Delta6B

      (Calpena et al., 2018)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The wing vein defects (vein thickening and deltas) characteristic for Dl6B mutants (reared at 29[o]C) are ameliorated by Scer\GAL4nub.PU-driven expression of jpKK107921.

      (Calpena et al., 2018)
      Xenogenetic Interactions
      Statement
      Reference

      The age-progressive retinal degeneration manifested by patchy depigmentation of the adult eye characteristic for flies expressing either Hsap\HTTQ93.ex1.Scer\UAS or Hsap\ATXN3CAG89.Scer\UAS.T:Ivir\HA1,T:Hsap\MYC,T:Zzzz\His6 under the control of Scer\GAL4GMR.PU is exacerbated by co-expression of jpKK107921.

      (Calpena et al., 2018)
      Complementation and Rescue Data
      Rescues

      jpKK107921/Scer\GAL4GMR.PU rescues jpUAS

      (Calpena et al., 2018)

      Scer\GAL4nub.PU/jpKK107921 rescues jpUAS

      (Calpena et al., 2018)
      Comments

      The adult eye phenotype (disrupted ommatidial lattice, supernumerary interommatidial bristles) induced by Scer\GAL4GMR.PU-driven expression of jpUAS as well as the reduced wing size observed upon expression with Scer\GAL4nub.PU is restored by co-expression of jpKK107921.

      (Calpena et al., 2018)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      jpKK107921
      Name Synonyms
      Secondary FlyBase IDs
        References (2)