FB2024_04 , released June 25, 2024
Allele: Dmel\jpUAS
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General Information
Symbol
Dmel\jpUAS
Species
D. melanogaster
Name
FlyBase ID
FBal0337964
Feature type
allele
Associated gene
Dmel\jp
Associated Insertion(s)
P{XP-U}jpUAS
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
FLPase
(Calpena et al., 2018)
Progenitor genotype
P{XP}jpd04563
(Calpena et al., 2018)
Associated Insertion(s)
P{XP-U}jpUAS
Cytology
Description

One of the two UAS regulatory elements present in the P{XP} element of the P{XP}jpd04563 insertion has been removed by excising the FRT cassette. The remaining UAS regulatory element is in the correct orientation to drive overexpression of jp in the presence of a GAL4 driver.

(Calpena et al., 2018)
Allele components
Component
Use(s)
Inserted element
P{XP-U}
Regulatory region(s)
UAS
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  cardiomyopathy
      CEA
      (Calpena et al., 2018)
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of jpUAS under the control of Scer\GAL4Mhc.PU yields viable adult flies with slightly reduced lifespan that also show rapid age-progressive decline in either climbing or flight ability compared to controls, although their indirect flight muscles (and mitochondria therein) do not display any obvious morphological defects even in aged flies. However, the diads in the muscles have aberrant diads with elongated sarcomeric reticulum and occasionally show abnormal positioning away from the myofibrils and embedded in the mitochondria.

      Scer\GAL4tin.cBa-driven expression of jpUAS results in reduced adult lifespan and median survival at 29[o]C compared to controls, and whereas the diastolic interval, systolic interval or heart period length and arrhythmia index in 1-week-old flies at 29[o]C are all comparable to controls, the end-systolic and end-diastolic are significantly increased and the fractional shortening percentage is decreased; the cardiac chambers are enlarged but the thickness of the heart wall is not significantly different from controls. The parallel alignment of transverse myofibrils in the heart tube in the areas surrounding the ostia in jpUAS-expressing flies is disturbed.

      Adult flies expressing jpUAS under the Scer\GAL4elav.PU driver have reduced survival rate and display significantly longer recovery times in bang-sensitive analyses at 4 weeks after eclosion (but not at 1 week post-eclosion). Scer\GAL4GMR.PU-driven expression leads to mildly disrupted ommatidial organization with some misshapen ommatidia and supernumerary bristles, the ommatidia also frequently contain excessive photoreceptor cells, but no depigmentation is observed even in aged flies.

      Scer\GAL4Act5C.PU-driven expression of jpUAS results in supernumerary vibrissae and sternopleural bristles, duplication of the anterior scutellar macrochaetae and wing vein deltas at the wing margin. Expression driven by Scer\GAL4rn-GAL4 or Scer\GAL4nub.PU also induces formation of wing vein deltas along with thickening of longitudinal veins and overall decrease in the wing blade size.

      (Calpena et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      jpUAS/Scer\GAL4nub.PU is an enhancer of wing vein phenotype of Delta6B

      (Calpena et al., 2018)
      Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The wing vein defects (vein thickening and deltas) characteristic for Dl6B mutants (reared at 29[o]C) are exacerbated further by Scer\GAL4nub.PU-driven expression of jpUAS.

      (Calpena et al., 2018)
      Xenogenetic Interactions
      Statement
      Reference

      The age-progressive retinal degeneration manifested by patchy depigmentation of the adult eye characteristic for flies expressing either Hsap\HTTQ93.ex1.Scer\UAS or Hsap\ATXN3CAG89.Scer\UAS.T:Ivir\HA1,T:Hsap\MYC,T:Zzzz\His6 under the control of Scer\GAL4GMR.PU is ameliorated by co-expression of jpUAS.

      (Calpena et al., 2018)
      Complementation and Rescue Data
      Rescued by

      jpUAS is rescued by jpKK107921/Scer\GAL4GMR.PU

      (Calpena et al., 2018)

      jpUAS is rescued by Scer\GAL4nub.PU/jpKK107921

      (Calpena et al., 2018)
      Comments

      The adult eye phenotype (disrupted ommatidial lattice, supernumerary interommatidial bristles) induced by Scer\GAL4GMR.PU-driven expression of jpUAS as well as the reduced wing size observed upon expression with Scer\GAL4nub.PU is restored by co-expression of jpKK107921.

      (Calpena et al., 2018)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      jpUAS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)