FlyBase Allele Report: Dmel\Ada2b[d842]

General Information

Symbol

Dmel\Ada2b^d842

Species

D. melanogaster

Name

FlyBase ID

FBal0239942

Feature type

allele

Associated gene

Dmel\Ada2b

Associated Insertion(s)

Carried in Construct

Also Known As

dAda2b^d842, Ada2b⁸⁴²

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Pankotai et al., 2005)

Progenitor genotype

P{EP}EP3412

(Pankotai et al., 2005)

Cytology

Description

Imprecise excision of the progenitor insertion has resulted in an 800bp deletion, removing the promoter and extending into the first intron of Ada2b.

(Pankotai et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile (with Ada2b¹), with Ada2b^PA

(Torres-Zelada et al., 2019)

fertile (with Ada2b¹), with Ada2b^PA, Ada2b^PB

(Torres-Zelada et al., 2019)

lethal (with Ada2b¹)

(Torres-Zelada et al., 2019)

lethal | pharate adult stage

(Pankotai et al., 2005)

lethal | pharate adult stage (with Df(3R)Ada2b^d52)

(Pankotai et al., 2005)

lethal | pupal stage P5

(Pankotai et al., 2005)

lethal | pupal stage P5 (with Df(3R)Ada2b^d52)

(Pankotai et al., 2005)

lethal - all die before end of pupal stage

(Zsindely et al., 2009)

some die during pupal stage

(Zsindely et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Ada2b^d842 follicle cell clones frequently show picnotic nuclei or frequently fail to show the expected gene amplification (BrdU-positive foci) normally observed in controls.

(Torres-Zelada et al., 2019)

Homozygotes undergo apparently normal development until the P5 stage, except that the larval and first stages of pupal development take slightly longer to complete than in their control siblings. At the P5 stage, the legs and head show reduced development compared to controls and 85% of the animals die. A small fraction of the mutants reach the pharate adult stage.

(Zsindely et al., 2009)

Homozygotes and Ada2b^d842/Df(3R)Ada2b^d52 transheterozygotes show 77% lethality at the P5 pupal stage, and only 23% reach the pharate adult stage. No animals emerge as adults.

In disc-derived body-parts, homozygous clones develop as frequently as control clones, but are significantly smaller than control clones. Homozygous clones on the abdomen are as large as control clones.

Heterozygous adults show a reduction in eye pigmentation compared to wild type.

Mutant imaginal discs exhibit significantly lower levels of apoptosis (as stained by Acridine Orange) when subjected to X-ray irradiation (with 4,000R) compared to wild-type discs.

(Pankotai et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Ada2b^d842/Ada2b^d842 is a non-suppressor of polytene chromosome puff phenotype of Fcp1^RNAi.UAS.WIZ, Scer\GAL4^hs.PB

(Ciurciu et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The puff in the 61C region induced by Scer\GAL4^hs.PB-mediated expression of P{UAS-Fcp1.dsRNA.WIZ}61C remains readily detectable in Ada2b^d842 homozygotes.

(Ciurciu et al., 2009)

The presence of p53^Scer\UAS.Ex, under the control of Scer\GAL4^GMR.PF does not affect the reduction in eye pigmentation observed in Ada2b^d842 heterozygotes.

(Pankotai et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Ada2b¹/Ada2b^d842 is rescued by Ada2b^PA/Ada2b^PB

(Torres-Zelada et al., 2019)

Partially rescued by

Ada2b¹/Ada2b^d842 is partially rescued by Ada2b^PA

(Torres-Zelada et al., 2019)

Ada2b^d842 is partially rescued by Ada2b^UASp.EGFP

(Pankotai et al., 2013)

Ada2b^d842 is partially rescued by Ada2b^UASp.S.EGFP

(Pankotai et al., 2013)

Ada2b^d842 is partially rescued by Ada2b^UAS.S/Scer\GAL4^da.G32

(Pankotai et al., 2013)

Ada2b^d842 is partially rescued by Scer\GAL4^Act.PU/Ada2b^UAS.cVa

(Vamos and Boros, 2012)

Ada2b^d842 is partially rescued by Scer\GAL4^Act.PU/Ada2a::Ada2b^2a2b.UAS

(Vamos and Boros, 2012)

Ada2b^d842 is partially rescued by Ada2b^UASp.EGFP

(Zsindely et al., 2009)

Ada2b^d842 is partially rescued by Scer\GAL4^Act5C.PU/Ada2b^UASp.EGFP

(Zsindely et al., 2009)

Not rescued by

Ada2b¹/Ada2b^d842 is not rescued by Ada2b^PB

(Torres-Zelada et al., 2019)

Ada2b^d842 is not rescued by Scer\GAL4^Act.PU/Ada2a^UAS.cVa

(Vamos and Boros, 2012)

Ada2b^d842 is not rescued by Scer\GAL4^Act.PU/Ada2a::Ada2b^2bS.2a.UAS

(Vamos and Boros, 2012)

Ada2b^d842 is not rescued by Scer\GAL4^Act.PU/Ada2a::Ada2b^2bM.2a.UAS

(Vamos and Boros, 2012)

Ada2b^d842 is not rescued by Ada2a::Ada2b^2bL.2a.UAS/Scer\GAL4^Act.PU

(Vamos and Boros, 2012)

Comments

10% of Ada2b^d842 mutants carrying a single copy of Ada2b^{Scer\UAS.P\T.S.T:Avic\GFP-EGFP} emerge as adults.

85% of Ada2b^d842 mutants carrying a single copy of Ada2b^{Scer\UAS.P\T.T:Avic\GFP-EGFP} emerge as adults.

40% of Ada2b^d842 mutants expressing Ada2b^Scer\UAS.S under the control of Scer\GAL4^da.G32 emerge as adults.

(Pankotai et al., 2013)

Expression of Ada2b^Scer\UAS.cVa under the control of Scer\GAL4^Act.PU partially rescues the pupal P5 stage lethality of Ada2b^d842 animals, supporting development to the pharate adult (P14) stage in 80% of cases.

Expression of Ada2a::Ada2b^{2a2b.Scer\UAS} under the control of Scer\GAL4^Act.PU partially rescues the pupal P5 stage lethality of Ada2b^d842 animals, with over 50% of the animals reaching the pharate adult (P14) stage. A small fraction of animals emerge as adults, but they die within 12 hours of hatching, with their wings still folded.

Expression of either Ada2a::Ada2b^{2bS.2a.Scer\UAS}, Ada2a::Ada2b^{2bM.2a.Scer\UAS}, Ada2a::Ada2b^{2bL.2a.Scer\UAS} or Ada2a^Scer\UAS.cVa under the control of Scer\GAL4^Act.PU fails to rescue the pupal P5 stage lethality of Ada2b^d842 animals.

(Vamos and Boros, 2012)

Ada2b^{Scer\UAS.P\T.T:Avic\GFP-EGFP} is able to produce an 80% phenotypic rescue of Ada2b^d842 mutants in the absence of a Scer\GAL4 driver. When expression of Ada2b^{Scer\UAS.P\T.T:Avic\GFP-EGFP} is driven by Scer\GAL4^Act5C.PU the same degree of rescue of Ada2b^d842 is seen as in the absence of the driver.

(Zsindely et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Ada2b⁸⁴²

(Torres-Zelada et al., 2022, Grau et al., 2008)

Ada2b^d842

ada2b⁸⁴²

(Torres-Zelada et al., 2019)

dAda2b^d842

(Pankotai et al., 2013, Vamos and Boros, 2012, Ciurciu et al., 2009, Zsindely et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (8)

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