FlyBase Allele Report: Dmel\Fcp1[RNAi.UAS.WIZ]

General Information

Symbol

Dmel\Fcp1^RNAi.UAS.WIZ

Species

D. melanogaster

Name

FlyBase ID

FBal0220826

Feature type

allele

Associated gene

Dmel\Fcp1

Associated Insertion(s)

Carried in Construct

P{UAS-Fcp1.RNAi.WIZ}

Also Known As

WIZ-fcp1i

Key Links

Transgenic product class

RNAi_reagent

(Tombácz et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Tombácz et al., 2009)

Mutagen

in vitro construct

(Schauer et al., 2009, Tombácz et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-Fcp1.RNAi.WIZ}

Cytology

Description

UAS regulatory sequences drive expression of a repeat of the Fcp1 region between 1271 and 2205 in head-to-head orientation.

(Tombácz et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Fcp1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death, with Scer\GAL4^Act5C.PU

(Schauer et al., 2009)

lethal, with Scer\GAL4^Act5C.PU

(Schauer et al., 2009)

lethal, with Scer\GAL4^da.G32

(Schauer et al., 2009)

lethal, with Scer\GAL4^en-e16E

(Schauer et al., 2009)

lethal, with Scer\GAL4^Tub.PU

(Schauer et al., 2009)

lethal | prepupal stage P1, with Scer\GAL4^Act5C.PU

(Tombácz et al., 2009)

lethal | third instar larval stage, with Scer\GAL4^Act5C.PU

(Tombácz et al., 2009)

lethal - all die before end of prepupal stage, with Scer\GAL4^Act5C.PI

(Ciurciu et al., 2009)

some die during third instar larval stage, with Scer\GAL4^Act5C.PI

(Ciurciu et al., 2009)

visible, with Scer\GAL4^en-e16E

(Schauer et al., 2009)

visible, with Scer\GAL4^GMR.PF

(Schauer et al., 2009)

visible, with Scer\GAL4^vg.PU

(Schauer et al., 2009)

wild-type, with Fcp1^c.UAS, Scer\GAL4^Act5C.PU

(Tombácz et al., 2009)

wild-type, with Fcp1^{UAS.g.Tag:FLAG}, Scer\GAL4^Act5C.PU

(Tombácz et al., 2009)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Schauer et al., 2009)

imaginal disc, with Scer\GAL4^Act5C.PU

(Schauer et al., 2009)

polytene chromosome puff, with Scer\GAL4^Act5C.PI

(Ciurciu et al., 2009)

polytene chromosome puff, with Scer\GAL4^hs.PB

(Ciurciu et al., 2009)

wing, with Scer\GAL4^en-e16E

(Schauer et al., 2009)

wing, with Scer\GAL4^vg.PU

(Schauer et al., 2009)

Detailed Description

Statement

Reference

100% of Scer\GAL4^Act5C.PI Fcp1^{dsRNA.Scer\UAS.WIZ} animals die in late L3 early pupa stages.

Scer\GAL4^Act5C.PI- or Scer\GAL4^hs.PB-mediated expression of P{UAS-Fcp1.dsRNA.WIZ}61C results in formation of a well-detectable puff in the 61C region.

(Ciurciu et al., 2009)

Expression of Fcp1^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^Act5C results in extensive cell death in the imaginal discs (assessed by acridine orange staining).

(Schauer et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Fcp1^RNAi.UAS.WIZ, Scer\GAL4^vg.PU has visible phenotype, enhanceable by p53^H159N.UAS.Ex, Scer\GAL4^vg.PU

(Schauer et al., 2009)

Fcp1^RNAi.UAS.WIZ, Scer\GAL4^en-e16E has lethal phenotype, enhanceable by p53^H159N.UAS.Ex, Scer\GAL4^en-e16E

(Schauer et al., 2009)

NOT Enhanced by

Statement

Reference

Fcp1^RNAi.UAS.WIZ, Scer\GAL4^en-e16E has lethal phenotype, non-enhanceable by mei-41^RT1

(Schauer et al., 2009)

Suppressed by

Statement

Reference

Fcp1^RNAi.UAS.WIZ, Scer\GAL4^vg.PU has visible phenotype, suppressible by p53^5A-1-4/p53^5A-1-4

(Schauer et al., 2009)

NOT suppressed by

Statement

Reference

Fcp1^RNAi.UAS.WIZ, Scer\GAL4^da.G32 has lethal phenotype, non-suppressible by mei-41^RT1

(Schauer et al., 2009)

Fcp1^RNAi.UAS.WIZ, Scer\GAL4^en-e16E has lethal phenotype, non-suppressible by mei-41^RT1

(Schauer et al., 2009)

Other

Statement

Reference

Fcp1^RNAi.UAS.WIZ, Scer\GAL4^Act5C.PU, Xlae\Fcp1^UASp.cTa has wild-type phenotype

(Tombácz et al., 2009)

Fcp1^RNAi.UAS.WIZ, Scer\GAL4^Act5C.PU, Xlae\Fcp1^D181E.UASp has lethal | third instar larval stage phenotype

(Tombácz et al., 2009)

Fcp1^RNAi.UAS.WIZ, Scer\GAL4^Act5C.PU, Xlae\Fcp1^D181E.UASp has lethal | prepupal stage P1 phenotype

(Tombácz et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Fcp1^RNAi.UAS.WIZ, Scer\GAL4^vg.PU has wing phenotype, enhanceable by p53^H159N.UAS.Ex, Scer\GAL4^vg.PU

(Schauer et al., 2009)

Suppressed by

Statement

Reference

Fcp1^RNAi.UAS.WIZ, Scer\GAL4^vg.PU has wing phenotype, suppressible by p53^5A-1-4/p53^5A-1-4

(Schauer et al., 2009)

NOT suppressed by

Statement

Reference

Fcp1^RNAi.UAS.WIZ, Scer\GAL4^Act5C.PI has polytene chromosome puff phenotype, non-suppressible by Polr2D^d189/Polr2D^d189

(Ciurciu et al., 2009)

Fcp1^RNAi.UAS.WIZ, Scer\GAL4^hs.PB has polytene chromosome puff phenotype, non-suppressible by Ada2b^d842/Ada2b^d842

(Ciurciu et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The puff in the 61C region induced by Scer\GAL4^hs.PB-mediated expression of P{UAS-Fcp1.dsRNA.WIZ}61C remains readily detectable in Ada2b^d842 homozygotes.

The puff in the 61C region induced by Scer\GAL4^Act5C.PI-mediated expression of P{UAS-Fcp1.dsRNA.WIZ}61C remains readily detectable in Rpb4^d189 homozygotes.

(Ciurciu et al., 2009)

The lethality of animals expressing Fcp1^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^en-e16E is shifted to an earlier phase if the animals are also co-expressing p53^{H159N.Scer\UAS.Ex}.

(Schauer et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Fcp1^RNAi.UAS.WIZ is rescued by Fcp1^c.UAS/Scer\GAL4^Act5C.PI

(Ciurciu et al., 2009)

Rescues

Scer\GAL4^Act5C.PI/Fcp1^RNAi.UAS.WIZ rescues Fcp1^c.UAS

(Ciurciu et al., 2009)

Comments

Scer\GAL4^Act5C.PI-mediated co-expression of both Fcp1^c.Scer\UAS and Fcp1^{dsRNA.Scer\UAS.WIZ} has no adverse effect and development is normal.

(Ciurciu et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Fcp1^RNAi.UAS.WIZ

Fcp1^{dsRNA.Scer\UAS.WIZ}

Fcp1^{dsRNA.UAS.WIZ}

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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