FlyBase Allele Report: Dmel\larp[S003108]

General Information

Symbol

Dmel\larp^S003108

Species

D. melanogaster

Name

FlyBase ID

FBal0241443

Feature type

allele

Associated gene

Dmel\larp

Associated Insertion(s)

P{lacW}larp^S003108

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Deak et al., 1997)

Progenitor genotype

Associated Insertion(s)

P{lacW}larp^S003108

Cytology

Description

P{lacW} insertion in the first intron.

(Ichihara et al., 2007)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle

(Blagden et al., 2009)

abnormal mitotic cell cycle (with larp^mtr-null)

(Blagden et al., 2009)

female sterile

(Ichihara et al., 2007)

female sterile (with larp^mtr-null)

(Blagden et al., 2009)

male sterile

(Ichihara et al., 2007)

male sterile (with larp^mtr-null)

(Blagden et al., 2009)

partially lethal - majority die | recessive

(Deak et al., 1997)

Phenotype Manifest In

centrosome & spermatocyte

(Ichihara et al., 2007)

embryo | embryonic stage 4 (with larp^mtr-null)

(Blagden et al., 2009)

mitochondrion & spermatocyte

(Ichihara et al., 2007)

Nebenkern

(Ichihara et al., 2007)

onion stage spermatid

(Ichihara et al., 2007)

spermatocyte

(Blagden et al., 2009)

spindle (with larp^mtr-null)

(Blagden et al., 2009)

spindle microtubule & spermatocyte

(Ichihara et al., 2007)

Detailed Description

Statement

Reference

71% of larp^S003108/larp^mtr-null females are sterile.

Eggs laid by larp^S003108/larp^mtr-null females show a number of defects during syncytial divisions, including chains of linked spindles, free centrosomes, multipolar spindles, chromosome segregation defects and a loss of synchrony throughout the syncytial divisions.

35.4% of homozygous spermatocytes show defects in meiosis.

(Blagden et al., 2009)

42.0% of onion stage spermatids show abnormalities in larp^mtrw males. Of these abnormal cells, 38.3% show abnormal cell numbers or size, 34.9% have abnormal Nebenkern numbers or size, 26.8% show a failure of cytokinesis and 38.1% have multiple abnormalities.

Mutants males show defects in spindle pole organisation in meiotic cells; abnormal cells are often seen in which a subset of spindle microtubules are not connected to spindle poles and fail to orient along the spindle axis at metaphase. Microtubules around the spindle poles appear not to be focused on the poles and there is a loss of centrosome integrity. The central spindle fails to form in some telophase cells, leading to a failure in cytokinesis. Some figures are seen where a chromosome mass is dislocated from a cytoplasmic space between poles.

The distribution of mitochondria during meiotic division is significantly affected in mutant males compared to wild type. In the mutant males, the mitochondria do not move into an interior region at anaphase and the mitochondria are sometimes partitioned unevenly during cytokinesis.

(Ichihara et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

larp^S003108 has abnormal meiotic cell cycle phenotype, enhanceable by pAbp[+]/pAbp^EP310

(Blagden et al., 2009)

Enhancer of

Statement

Reference

larp^S003108/larp[+] is an enhancer of abnormal meiotic cell cycle phenotype of pAbp^EP310

(Blagden et al., 2009)

Other

Statement

Reference

larp^S003108/larp[+], pAbp^EP310 has abnormal meiotic cell cycle | dominant phenotype

(Blagden et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

larp^S003108 has spermatocyte phenotype, enhanceable by pAbp[+]/pAbp^EP310

(Blagden et al., 2009)

Enhancer of

Statement

Reference

larp^S003108/larp[+] is an enhancer of spermatocyte phenotype of pAbp^EP310

(Blagden et al., 2009)

Other

Statement

Reference

larp^S003108/larp[+], pAbp^EP310 has spermatocyte phenotype

(Blagden et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

16% of pAbp^EP310/+ ; larp^S003108/+ double heterozygous spermatocytes show defects in meiosis.

The frequency of homozygous pAbp^EP310 spermatocytes that show defects in meiosis (17%) is increased by larp^S003108/+ (to 29.3%).

The frequency of homozygous larp^S003108 spermatocytes that show defects in meiosis (35.4%) is increased by pAbp^EP310/+ (to 48%).

(Blagden et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

larp^S003108 is rescued by larp^cIa

(Ichihara et al., 2007)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Allelic series (severity of mutant alleles): larp^mtr-null > larp^S003108 > larp^S022529 > larp⁰⁶⁴⁸⁷.

(Blagden et al., 2009)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

0031/08

(Deak et al., 1997)

l(3)S003108^S003108

l(3)S003108^l(3)S003108

(Blagden et al., 2009)

larp^S003108

larp<up>mtr w</up>

(Blagden et al., 2009)

larp^mtrw

(Ichihara et al., 2007)

mtr^w

(Ichihara et al., 2007)

Name Synonyms

Secondary FlyBase IDs

FBal0220947
FBal0130100

References (3)

Report Sections

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