FlyBase Allele Report: Dmel\larp[mtr-null]

General Information

Symbol

Dmel\larp^mtr-null

Species

D. melanogaster

Name

FlyBase ID

FBal0241445

Feature type

allele

Associated gene

Dmel\larp

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

FLPase

Nature of the Allele

Allele class

Mutagen

FLPase

(Blagden et al., 2009)

Progenitor genotype

PBac{WH}f03059

(Blagden et al., 2009)

P{XP}larp^d00350

(Blagden et al., 2009)

Cytology

Description

Recombination between the two progenitor insertion has resulted in a deletion of the intervening sequence, deleting larp sequences.

(Blagden et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle

(Blagden et al., 2009)

abnormal mitotic cell cycle (with larp^S003108)

(Blagden et al., 2009)

abnormal mitotic cell cycle (with larp^S022529)

(Blagden et al., 2009)

female sterile (with larp^S003108)

(Blagden et al., 2009)

female sterile (with larp^S022529)

(Blagden et al., 2009)

female sterile | recessive

(Blagden et al., 2009)

male sterile (with larp⁰⁶⁴⁸⁷)

(Blagden et al., 2009)

male sterile (with larp^S003108)

(Blagden et al., 2009)

male sterile (with larp^S022529)

(Blagden et al., 2009)

male sterile | recessive

(Blagden et al., 2009)

viable

(Blagden et al., 2009)

visible

(Blagden et al., 2009)

Phenotype Manifest In

centrosome (with larp^S022529)

(Blagden et al., 2009)

chaeta

(Blagden et al., 2009)

embryo | embryonic stage 4 (with larp^S003108)

(Blagden et al., 2009)

embryo | embryonic stage 4 (with larp^S022529)

(Blagden et al., 2009)

eye

(Blagden et al., 2009)

mitochondrion

(Zhang et al., 2016)

ovary

(Zhang et al., 2016)

spermatocyte

(Blagden et al., 2009)

spindle (with larp^S003108)

(Blagden et al., 2009)

spindle (with larp^S022529)

(Blagden et al., 2009)

Detailed Description

Statement

Reference

Replication of mtDNA is severely impaired in larp^mtr-null mutant ovaries.

(Zhang et al., 2016)

Homozygotes have shorter and thinner bristles than normal and some bristles are occasionally missing. The mutant flies also have a slight rough eye phenotype.

71% of larp^S003108/larp^mtr-null females are sterile.

45% of larp^S022529/larp^mtr-null females are sterile.

6% of larp⁰⁶⁴⁸⁷/larp^mtr-null females are sterile.

Homozygous females do not lay eggs.

Eggs laid by larp^S003108/larp^mtr-null females show a number of defects during syncytial divisions, including chains of linked spindles, free centrosomes, multipolar spindles, chromosome segregation defects and a loss of synchrony throughout the syncytial divisions.

Eggs laid by larp^S022529/larp^mtr-null females show a number of defects during syncytial divisions, including chains of linked spindles and free centrosomes. Time-lapse imaging shows that centrosomes can be seen to successfully duplicate following the previous division cycle, however the movement of the centrosomes around the nuclear membrane is hindered. Nonetheless, a spindle forms between the two groups of centrosomes. Nuclear spacing is also disrupted.

48.4% of homozygous spermatocytes show defects in meiosis.

(Blagden et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

larp^mtr-null/larp[+] is an enhancer of abnormal meiotic cell cycle phenotype of pAbp^EP310

(Blagden et al., 2009)

Other

Statement

Reference

larp^mtr-null/larp[+], pAbp^EP310 has abnormal meiotic cell cycle | dominant phenotype

(Blagden et al., 2009)

Phenotype Manifest In

Enhancer of

Statement

Reference

larp^mtr-null/larp[+] is an enhancer of spermatocyte phenotype of pAbp^EP310

(Blagden et al., 2009)

Other

Statement

Reference

larp^mtr-null/larp[+], pAbp^EP310 has spermatocyte phenotype

(Blagden et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

23% of pAbp^EP310/+ ; larp^mtr-null/+ double heterozygous spermatocytes show defects in meiosis.

The frequency of homozygous pAbp^EP310 spermatocytes that show defects in meiosis (17%) is increased by larp^mtr-null/+ (to 31.4%).

(Blagden et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Allelic series (severity of mutant alleles): larp^mtr-null > larp^S003108 > larp^S022529 > larp⁰⁶⁴⁸⁷.

(Blagden et al., 2009)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

larp<up>mtr null</up>

(Blagden et al., 2009)

larp^mtr-null

(Zhang et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

Open Close