FlyBase Allele Report: Dmel\mst[JF03280]

The expression of mst^JF03280 under the control of Scer\GAL4^Mef2.PU leads to short-lived adults, who exhibit a significant decrease in food intake, a near absence of gut contractions, a decreased movement of food through the intestinal tract (and a near absence of fecal deposition), a progressive abdominal dilation, a progressive and significant intestine dilation, a severe increase in gut permeability, increased signs of infection (i.e. melanization marks), and progressively and severely disturbed circular and longitudinal intestinal muscle fibers, associated with a disrupted sarcomere structure and weakened actin filaments, but no changes in sensitivity to food (measured by the frequency of proboscis extension responses to sucrose), as compared to controls; the visceral muscle fibers of old individuals also exhibit a significant increase in apoptosis and disrupted mitochondria structure, as compared to controls. Expression under the combined control of Scer\GAL4^Mef2.PU and Gal80[ts] (for adulthood-only expression) or Scer\GAL4^Mef2.PU and Scer\GAL80^nSyb.PS still leads to a dilated intestine, as compared to controls.

Adults expressing mst^JF03280 under the control of Scer\GAL4^how-24B leads to short-lived adults, who exhibit a significant decrease in food intake, a dilated intestine, a near absence of gut contractions, and severely disturbed circular and longitudinal intestinal muscle fibers, as compared to controls. Expression under the control of Scer\GAL4^tey-5053A leads to a small but significant increase in food intake, a mildly thinner intestine and to a moderately disturbed longitudinal (but not circular) intestinal muscle fibers, as compared to controls. Expression under the control of Scer\GAL4^{Myo31DF-NP0001} has no significant effect in feeding behavior, in intestine diameter or in circular and longitudinal intestinal muscle fibers, as compared to controls.

The expression of mst^JF03280 under the control of Scer\GAL4^Act5C.PI, but not Scer\GAL4^Cg.PA or Scer\GAL4^nSyb.PS, leads to adults exhibiting a significant decrease in food intake, as compared to controls.

The expression of mst^JF03280 under the control of Scer\GAL4^Mhc.PU has no obvious effect on the locomotor activity, intestine diameter or skeletal muscle structures in adults, as compared to controls.

(Min et al., 2017)

External Data

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Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Mef2.PU, mst^JF03280 has abnormal size | adult stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^Mef2.PU

(Min et al., 2017)

Scer\GAL4^Mef2.PU, mst^JF03280 has abnormal feeding behavior | adult stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^Mef2.PU

(Min et al., 2017)

Scer\GAL4^Mef2.PU, mst^JF03280 has short lived phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^Mef2.PU

(Min et al., 2017)

Scer\GAL4^Mef2.PU, mst^JF03280 has abnormal size | adult stage phenotype, suppressible by Act79B^UASp.GFP, Scer\GAL4^Mef2.PU

(Min et al., 2017)

NOT Suppressor of

Statement

Reference

mst^JF03280, Scer\GAL4^Mef2.PU is a non-suppressor of lethal phenotype of Act42A^UASp.GFP, Scer\GAL4^Mef2.PU

(Min et al., 2017)

mst^JF03280, Scer\GAL4^Mef2.PU is a non-suppressor of lethal phenotype of Act57B^UASp.GFP, Scer\GAL4^Mef2.PU

(Min et al., 2017)

mst^JF03280, Scer\GAL4^Mef2.PU is a non-suppressor of lethal phenotype of Act87E^UASp.GFP, Scer\GAL4^Mef2.PU

(Min et al., 2017)

mst^JF03280, Scer\GAL4^Mef2.PU is a non-suppressor of lethal phenotype of Act88F^UASp.GFP, Scer\GAL4^Mef2.PU

(Min et al., 2017)

mst^JF03280, Scer\GAL4^Mef2.PU is a non-suppressor of lethal phenotype of Act5C^UASp.R.GFP, Scer\GAL4^Mef2.PU

(Min et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Mef2.PU, mst^JF03280 has adult midgut phenotype, suppressible by Act79B^UASp.GFP, Scer\GAL4^Mef2.PU

(Min et al., 2017)

Scer\GAL4^Mef2.PU, mst^JF03280 has filamentous actin | adult stage phenotype, suppressible by Act79B^UASp.GFP, Scer\GAL4^Mef2.PU

(Min et al., 2017)

Scer\GAL4^Mef2.PU, mst^JF03280 has adult midgut phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^Mef2.PU

(Min et al., 2017)

Scer\GAL4^Mef2.PU, mst^JF03280 has filamentous actin | adult stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^Mef2.PU

(Min et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The lethality induced by the expression of Act42A^{Scer\UAS.P\T.T:Avic\GFP}, Act57B^{Scer\UAS.P\T.T:Avic\GFP}, Act87E^{Scer\UAS.P\T.T:Avic\GFP}, Act88F^{Scer\UAS.P\T.T:Avic\GFP}, or Act5C^{Scer\UAS.P\T.R.T:Avic\GFP} under the control of Scer\GAL4^Mef2.PU is not suppressed by the co-expression of mst^JF03280.

The co-expression of Act79B^{Scer\UAS.P\T.T:Avic\GFP} fully suppresses the dilated intestine and the visceral muscle actin defects and partially suppresses the decreased lifespan observed in adults expressing mst^JF03280 under the control of Scer\GAL4^Mef2.PU.

(Min et al., 2017)

Xenogenetic Interactions

Statement

Reference

The decreased lifespan, the decreased food intake, the decreased gut contractions, the dilated intestine, and the visceral muscle structure defects (including in actin filaments) observed in adults expressing mst^JF03280 under the control of Scer\GAL4^Mef2.PU are rescued by the co-expression of BacA\p35^Scer\UAS.cHa.

(Min et al., 2017)

Complementation and Rescue Data

Rescued by

mst^JF03280 is rescued by mst^UAS.ORF/Scer\GAL4^Mef2.PU

(Min et al., 2017)

mst^JF03280 is rescued by Scer\GAL4^Mef2.PU/mst^{Δ2.UAS.Tag:HA}

(Min et al., 2017)

Partially rescued by

mst^JF03280 is partially rescued by Scer\GAL4^Mef2.PU/mst^UAS.GFP

(Min et al., 2017)

Not rescued by

mst^JF03280 is not rescued by mst^{Δ1.UAS.Tag:HA}/Scer\GAL4^Mef2.PU

(Min et al., 2017)

mst^JF03280 is not rescued by Scer\GAL4^Mef2.PU/mst^{Δ3.UAS.Tag:HA}

(Min et al., 2017)

Comments

The decreased lifespan, the decreased food intake, the decreased gut contractions, the dilated intestine, and the visceral muscle structure defects observed in adults expressing mst^JF03280 under the control of Scer\GAL4^Mef2.PU are rescued by the co-expression of mst^Scer\UAS.ORF.

The decreased lifespan observed in adults expression of mst^JF03280 under the control of Scer\GAL4^Mef2.PU is fully rescued by the co-expression of mst^{Δ2.Scer\UAS.T:Ivir\HA1}, is partially rescued by the co-expression of mst^{Scer\UAS.T:Avic\GFP}, and is not rescued by the co-expression of either mst^{Δ1.Scer\UAS.T:Ivir\HA1} or mst^{Δ3.Scer\UAS.T:Ivir\HA1}.

(Min et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

29601

y¹ v¹; P{TRiP.JF03280}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

mst^JF03280

Name Synonyms

Secondary FlyBase IDs

References (3)

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