FlyBase Allele Report: Dmel\Blos1[ex2]

General Information

Symbol

Dmel\Blos1^ex2

Species

D. melanogaster

Name

FlyBase ID

FBal0242577

Feature type

allele

Associated gene

Dmel\Blos1

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Cheli et al., 2010)

Progenitor genotype

P{EPgy2}Blos1^EY06269

(Cheli et al., 2010)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a deletion which removes sequences from -56 to +38 relative to the ATG start site.

(Cheli et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of schizophrenia

CEA

(Mullin et al., 2015)

CEA with Dysb^e01028

(Mullin et al., 2015)

model of Hermansky-Pudlak syndrome

CEA

(Cheli et al., 2010)

model of metal metabolism disorder

CEA

(Gokhale et al., 2015)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Hermansky-Pudlak syndrome

is exacerbated by Rab32^ltd-1

(Cheli et al., 2010)

is exacerbated by cm¹

(Cheli et al., 2010)

is exacerbated by g²

(Cheli et al., 2010)

is exacerbated by aux^{CJ.UAS.mRFP(Unk)}

(Cheli et al., 2010)

is ameliorated by Rab11^d01994

(Cheli et al., 2010)

is exacerbated by aux^{FL.UAS.mRFP(Unk)}

(Cheli et al., 2010)

is exacerbated by rb¹

(Cheli et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

photoreceptor cell pigment granule

(Cheli et al., 2010)

synapse | wandering third instar larval stage

(Chen et al., 2017)

Detailed Description

Statement

Reference

Blos1^ex2 homozygous wandering third instar larvae display a significantly increased rate of depletion and a significantly decreased rate of recovery of the synaptic vesicle pool upon high-frequency stimulation of the neuromuscular junction, as compared to controls.

(Chen et al., 2017)

Both homozygous and heterozygous Blos1^ex2 mutant flies have increased miniature excitatory junction potential amplitude and a correspondingly lower quantal content.

Blos1^ex2 mutant synaptic vesicles are similar in size to controls.

Homozygous Blos1^ex2 mutant flies exhibit deficits in short term olfactory habituation. This phenotype is not observed in either heterozygote.

(Mullin et al., 2015)

blos1^ex2 mutants exhibit normal homeostatic compensation following application of PhTx, indicating that blos1 is not involved in homeostatic plasticity.

(Dickman et al., 2012)

Mutant flies have reduced red pigment in the eye compared to controls.

(Cheli et al., 2010)

Homozygous flies show a reduction in both brown and red pigment levels in the retina, in the absence of any gross anatomical abnormality. There are no defects in the organisation of the ommatidia or in the number of photoreceptors. The number and electron density of pigment granules within mutant pigment cells is reduced, and the remaining pigment granules have an abnormal morphology.

The average amplitude of spontaneous miniature excitatory junction potentials (mEJPs) at the neuromuscular junction (NMJ) is approximately 24% larger than that of controls in mutant third instar larvae. The mutant NMJ shows a difference in muscle resting potential compared to wild type, but this difference is too small to account for the difference in mEJP amplitude. The mutant NMJs show no significant difference in the average muscle input resistance, the frequency of mEJP events, the average amplitude of evoked EJPs or the estimated quantal content (EJP/mEJP amplitude ration) compared to controls.

The number of mutant males engaged in male-to-male courtship behaviour during a 10 minute period is significantly higher than that of wild-type flies.

Homozygous flies show locomotor hyperactivity, including rapid jumping and running as well as interrupted flight. The number of physical contacts between flies that occur during a 10 minute period is increased in homozygous flies compared to controls.

(Cheli et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Blos1^ex2 has abnormal eye color phenotype, enhanceable by aux^UAS.cHa/Scer\GAL4^GMR.PF

(Cheli et al., 2010)

Blos1^ex2 has abnormal eye color phenotype, enhanceable by rb¹

(Cheli et al., 2010)

Blos1^ex2 has abnormal eye color phenotype, enhanceable by aux^{FL.UAS.mRFP(Unk)}/Scer\GAL4^GMR.PF

(Cheli et al., 2010)

Blos1^ex2 has abnormal eye color phenotype, enhanceable by aux^{CJ.UAS.mRFP(Unk)}/Scer\GAL4^GMR.PF

(Cheli et al., 2010)

Blos1^ex2 has abnormal eye color phenotype, enhanceable by cm¹

(Cheli et al., 2010)

Blos1^ex2 has abnormal eye color phenotype, enhanceable by g²

(Cheli et al., 2010)

Blos1^ex2 has abnormal eye color phenotype, enhanceable by Rab32^ltd-1

(Cheli et al., 2010)

NOT Enhanced by

Statement

Reference

Blos1^ex2 has abnormal eye color phenotype, non-enhanceable by Scer\GAL4^GMR.PF/aux^{ΔC.UAS.mRFP(Unk)}

(Cheli et al., 2010)

Blos1^ex2 has abnormal eye color phenotype, non-enhanceable by Scer\GAL4^GMR.PF/aux^{CJ.DLL.DPF.UAS.mRFP(Unk)}

(Cheli et al., 2010)

Blos1^ex2 has abnormal eye color phenotype, non-enhanceable by aux^{CJ.DPF.UAS.mRFP(Unk)}/Scer\GAL4^GMR.PF

(Cheli et al., 2010)

Suppressed by

Statement

Reference

Blos1^ex2 has abnormal courtship behavior | male phenotype, suppressible by w^unspecified

(Cheli et al., 2010)

Blos1^ex2 has abnormal eye color phenotype, suppressible by Scer\GAL4^GMR.PF/Rab11^d01994

(Cheli et al., 2010)

Enhancer of

Statement

Reference

Blos1^ex2 is an enhancer of abnormal eye color phenotype of rb¹

(Cheli et al., 2010)

Blos1^ex2 is an enhancer of abnormal eye color phenotype of cm¹

(Cheli et al., 2010)

Blos1^ex2 is an enhancer of abnormal eye color phenotype of g²

(Cheli et al., 2010)

Blos1^ex2 is an enhancer of abnormal eye color phenotype of Rab32^ltd-1

(Cheli et al., 2010)

NOT Enhancer of

Statement

Reference

Blos1^ex2 is a non-enhancer of abnormal eye color phenotype of ca¹

(Cheli et al., 2010)

Blos1^ex2 is a non-enhancer of abnormal eye color phenotype of p^p

(Cheli et al., 2010)

NOT Suppressor of

Statement

Reference

Blos1^ex2 is a non-suppressor of abnormal eye color phenotype of ca¹

(Cheli et al., 2010)

Blos1^ex2 is a non-suppressor of abnormal eye color phenotype of p^p

(Cheli et al., 2010)

Other

Statement

Reference

Blos1[+]/Blos1^ex2, Dysb^e01028 has abnormal learning phenotype

(Mullin et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Dysb^e01028 Blos1^ex2 double heterozygotes exhibit deficits in short term olfactory habituation. This phenotype is not observed in either heterozygote alone.

(Mullin et al., 2015)

Expression of aux^Scer\UAS.cHa under the control of Scer\GAL4^GMR.PF enhances the loss of red eye pigment that is seen in blos1^ex2 flies.

(Cheli et al., 2010)

The increased male-to-male courtship behaviour of blos1^ex2 males is suppressed by w^unspecified.

The reduction in red pigment in the eye seen in rb¹ blos1^ex2, cm¹ blos1^ex2, ltd¹ blos1^ex2 and g² blos1^ex2 double mutants is more severe than that seen in either single mutant.

The reduced red eye pigment phenotype of ca¹ blos1^ex2 double mutants is indistinguishable from that of ca¹ single mutants.

The reduced red eye pigment phenotype of p^p blos1^ex2 double mutants is indistinguishable from that of p^p single mutants.

Expression of one of P{EPgy2}EY16920, CG9139^EP681a, PBac{WH}CG5342^f05247, P{EPgy2}EY00704, P{EPgy2}CG5903^EY11938 or CG17184^EY11874 under the control of Scer\GAL4^GMR.PF has no effect on the reduced red eye pigment phenotype of blos1^ex2 flies.

Expression of Rab11^d01994 under the control of Scer\GAL4^GMR.PF partially suppresses the reduced red eye pigment phenotype of blos1^ex2 flies.

The loss of red eye pigment that is seen in blos1^ex2 flies is enhanced by expression of one of aux^{FL.Scer\UAS.T:Disc\RFP-mRFP} or aux^{CJ.Scer\UAS.T:Disc\RFP-mRFP} under the control of Scer\GAL4^GMR.PF.

Expression of aux^{ΔC.Scer\UAS.T:Disc\RFP-mRFP} or aux^{CJ.DLL.DPF.Scer\UAS.T:Disc\RFP-mRFP} under the control of Scer\GAL4^GMR.PF does not enhance the loss of red eye pigment that is seen in blos1^ex2 flies.

(Cheli et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Blos1^ex2 is rescued by Scer\GAL4^GMR.PF/Blos1^UAS.cCa

(Cheli et al., 2010)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Blos1^ex2

(Lund et al., 2021, Mullin et al., 2015)

blos1^ex2

(Chen et al., 2017, Gokhale et al., 2015, Dickman et al., 2012, Cheli et al., 2010, Cheli et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (7)

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