FlyBase Allele Report: Dmel\Diap2[7c]

General Information

Symbol

Dmel\Diap2^7c

Species

D. melanogaster

Name

FlyBase ID

FBal0243966

Feature type

allele

Associated gene

Dmel\Diap2

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Leulier et al., 2006)

Mutagen

P-element activity

(Leulier et al., 2006)

Progenitor genotype

P{EP}Diap2^G2326

(Leulier et al., 2006)

Cytology

Description

Imprecise excision of the progenitor insertion resulting in a 1357 bp genomic deletion removing the start codon and the first 735 nt into the coding region of Iap2. The resultant protein lacks exon one and most of exon two.

(Leulier et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:15,933,456..15,934,813 [-]

Comment:

1,357 bp deletion resulting from the imprecise excision of P{EP}Diap2^G2326 maps to 2R: 11449676..11448319 in Release 4 coordinates.

(Leulier et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

wing disc

(Ribeiro et al., 2007)

wing disc (with Df(2R)Exel7138)

(Ribeiro et al., 2007)

Detailed Description

Statement

Reference

As in wild type, Iap2^7c mutant bursCCAP neurons undergo programmed cell death shortly after eclosion.

(Lee et al., 2013)

Mutant flies show greatly reduced survival compared to wild type after septic injury with Erwinia carotovora carotovora 15.

(Meinander et al., 2012)

Iap2^7c/Df(2R)Exel7138 or Iap2^7c homozygous third instar larvae subjected to sublethal doses of x-ray irradiation (1.5 Gy) display a marked increase in wing imaginal disc apoptosis compared to wild-type larvae.

(Ribeiro et al., 2007)

86% of Iap2^7c/Df(2R)Exel7138 animals survive embryogenesis and develop normally, however animals are acutely sensitive to infections. Iap2^7c/Df(2R)Exel7138 animals are highly susceptible to infection by gram-negative bacteria, but not to gram-positive bacteria and fungi. Iap2^7c/Df(2R)Exel7138 flies die within 25 hours of septic injury with gram-negative bacteria, and almost identical susceptibility is observed for Iap2^7c, or Iap2^7c/Iap2^7a transheterozygous mutant animals.

(Leulier et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Diap2^7c is rescued by Scer\GAL4^da.G32/Diap2^UAS.cLa

(Meinander et al., 2012)

Diap2^7c is rescued by Scer\GAL4^c564/Diap2^UAS.cMb

(Meinander et al., 2012)

Diap2^7c is rescued by Diap2^BIR2mut.UAS/Scer\GAL4^c564

(Meinander et al., 2012)

Diap2^7c is rescued by Diap2^BIR3mut.UAS/Scer\GAL4^c564

(Meinander et al., 2012)

Diap2^7c is rescued by Diap2^+t3.3

(Ribeiro et al., 2007)

Diap2^7c is rescued by Diap2^UAS.cLa

(Leulier et al., 2006)

Diap2^7c is rescued by Scer\GAL4^Act5C.PU/Diap2^UAS.cLa

(Leulier et al., 2006)

Diap2^7c is rescued by Scer\GAL4^da.G32/Diap2^UAS.cLa

(Leulier et al., 2006)

Not rescued by

Diap2^7c is not rescued by Scer\GAL4^da.G32/Diap2^RINGmut.UAS

(Meinander et al., 2012)

Diap2^7c is not rescued by Scer\GAL4^c564/Diap2^BIR23mut.UAS

(Meinander et al., 2012)

Comments

Expression of Iap2^{RINGmut.Scer\UAS} under the control of Scer\GAL4^da.G32 fails to rescue the reduced survival after septic injury with Erwinia carotovora carotovora which is seen in Iap2^7c flies.

Expression of Iap2^Scer\UAS.cLa under the control of Scer\GAL4^da.G32 rescues the reduced survival after septic injury with Erwinia carotovora carotovora 15 which is seen in Iap2^7c flies.

Expression of either Iap2^{Scer\UAS.attB}, Iap2^{BIR2mut.Scer\UAS} or Iap2^{BIR3mut.Scer\UAS} under the control of Scer\GAL4^c564 rescues the reduced survival after septic injury with Erwinia carotovora carotovora 15 which is seen in Iap2^7c flies.

Expression of Iap2^{BIR23mut.Scer\UAS} under the control of Scer\GAL4^da.G32 fails to rescue the reduced survival after septic injury with Erwinia carotovora carotovora 15 which is seen in Iap2^7c flies.

(Meinander et al., 2012)

Enhanced sensitivity of Iap2^7c homozygotes to x-ray irradiation is rescued by Iap2^+t3.3.

(Ribeiro et al., 2007)

Leaky, low-level expression of Iap2^Scer\UAS.cLa in a Iap2^7c mutant background is sufficient to completely rescue the immunodeficient phenotype observed in Iap2^7c mutant animals in response to infection by gram-negative bacteria.

Iap2^7c mutant animals expressing Iap2^Scer\UAS.cLa, under the control of Scer\GAL4^Act5C or Scer\GAL4^da.G32, are fully resistant to infection by gram-negative bacterium and survive microbial exposure at a similar rate to wild-type.

(Leulier et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

55725

w^*; Diap2^7c

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Diap2^7C

(Nandy et al., 2018)

Diap2^7c

(Kietz et al., 2022, Chen et al., 2017, Tavignot et al., 2017, Meinander et al., 2012)

Iap2^7c

diap2^7c

(Lee et al., 2013, Paquette et al., 2010, Ribeiro et al., 2007, Leulier et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (9)

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