FlyBase Allele Report: Scer\GAL4[Gyc89Da.1.9]

FB2024_03 , released June 25, 2024

FB2024_03 , released June 25, 2024

Allele: Scer\GAL4^Gyc89Da.1.9

General Information

Symbol

Scer\GAL4^Gyc89Da.1.9

Species

S. cerevisiae

Name

FlyBase ID

FBal0247196

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

P{Gyc89Da-GAL4.1.9}

Also Known As

Gyc-89Da^GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Morton et al., 2008)

Progenitor genotype

Carried in construct

P{Gyc89Da-GAL4.1.9}

Cytology

Description

A 1931bp fragment from the Gyc89Da promoter region (including the 5' UTR and first intron) is fused upstream of sequence encoding a GAL4 driver.

(Morton et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

gustatory receptor neuron | subset

(Vermehren-Schmaedick et al., 2011)

embryonic/larval olfactory receptor neuron | subset

(Vermehren-Schmaedick et al., 2011)

adult stage | female

gustatory receptor neuron | subset of labellum

Comment: 17 to 19 neurons

(Vermehren-Schmaedick et al., 2011)

adult stage | male

gustatory receptor neuron | subset of labellum

Comment: 10 to 11 neurons

(Vermehren-Schmaedick et al., 2011)

Additional Information

Statement

Reference

ScerGAL4^Gyc-89Da.1.9 is expressed in a subset of the gustatory receptor neurons that innervate the labellar sensilla in third instar larvae and in a few olfactory neurons. In the adult, it is expressed in the gustatory neurons of the labellum, with more neurons being labelled in females than in males. Three of these gustatory neurons in the lateral region of the labellum co-express ScerGAL4^Gyc-89Db.3.4. In addition, 11 of the 12 gustatory neurons that express ScerGAL4^Gyc-89Da.1.9 co-express DiscRFP^Gr66a.PD, a marker for bitter-sensitive neurons. ScerGAL4^Gyc-89Da.1.9 is found in a subset of the gustatory receptor neurons of the anterior wing margin and in the three most distal segments of the prothoracic leg.

(Vermehren-Schmaedick et al., 2011)

Marker for

Reflects expression of

(Vermehren-Schmaedick et al., 2011)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eclosion, with Ctet\tetX^TNT-LC.UAS

(Morton et al., 2008)

abnormal stress response | third instar larval stage, with Btau\PDE5A^UAS.cBa

(Vermehren-Schmaedick et al., 2010)

abnormal stress response | third instar larval stage, with CngA^KK108314

(Vermehren-Schmaedick et al., 2010)

abnormal stress response | third instar larval stage, with Gyc88E^GD11230

(Vermehren-Schmaedick et al., 2010)

abnormal taste perception, with Btau\PDE5A^UAS.cBa

(Vermehren-Schmaedick et al., 2011)

abnormal taste perception, with Gyc88E^GD11230

(Vermehren-Schmaedick et al., 2011)

lethal | first instar larval stage | heat sensitive, with Ctet\tetX^TNT-LC.UAS, Scer\GAL80^ts.αTub84B

(Morton et al., 2008)

lethal | late third instar larval stage, with Ctet\tetX^TNT-LC.UAS

(Morton et al., 2008)

lethal | pharate adult stage | heat sensitive, with Ctet\tetX^TNT-LC.UAS, Scer\GAL80^ts.αTub84B

(Morton et al., 2008)

lethal | pharate adult stage P15, with Ctet\tetX^TNT-LC.UAS

(Morton et al., 2008)

viable, with Btau\PDE5A^UAS.cBa

(Vermehren-Schmaedick et al., 2010, Morton et al., 2008)

viable, with Ctet\tetX^IMPTNT-V.UAS

(Morton et al., 2008)

viable, with Gyc88E^GD11230

(Vermehren-Schmaedick et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Gyc89Db^UAS.cVa/Scer\GAL4^Gyc89Da.1.9 is a suppressor of abnormal taste perception phenotype of Gyc89Da^e01821

(Vermehren-Schmaedick et al., 2011)

Gyc89Db^UAS.cVa/Scer\GAL4^Gyc89Da.1.9 is a suppressor of abnormal stress response | third instar larval stage phenotype of Gyc89Da^e01821

(Vermehren-Schmaedick et al., 2010)

Gyc89Da^UAS.cVa/Scer\GAL4^Gyc89Da.1.9 is a suppressor of abnormal stress response | third instar larval stage phenotype of Gyc89Db^MB03197

(Vermehren-Schmaedick et al., 2010)

NOT Suppressor of

Statement

Reference

Gyc89Da^UAS.cVa/Scer\GAL4^Gyc89Da.1.9 is a non-suppressor of abnormal taste perception phenotype of Gyc89Db^MB03197

(Vermehren-Schmaedick et al., 2011)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Gyc89Da^UAS.cVa/Scer\GAL4^Gyc89Da.1.9 rescues Gyc89Da^e01821

(Vermehren-Schmaedick et al., 2011)

Gyc89Da^UAS.cVa/Scer\GAL4^Gyc89Da.1.9 rescues Gyc89Da^e01821

(Vermehren-Schmaedick et al., 2010)

Gyc89Db^UAS.cVa/Scer\GAL4^Gyc89Da.1.9 rescues Gyc89Db^MB03197

(Vermehren-Schmaedick et al., 2010)

Gyc88E^UAS.cVa/Scer\GAL4^Gyc89Da.1.9 rescues Gyc88E^S451F/Gyc88E^V474M

(Vermehren-Schmaedick et al., 2010)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

93110

y¹ w^*; P{Gyc89Da-GAL4.1.9}2

93111

y¹ w^*; P{Gyc89Da-GAL4.1.9}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Gyc-89Da^GAL4

(Vermehren-Schmaedick et al., 2010)

Scer\GAL4^Gyc-89Da.1.9

Scer\GAL4^Gyc89Da.1.9

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections