FlyBase Allele Report: Dmel\Sin3A[RNAi.UAS.WIZ]

General Information

Symbol

Dmel\Sin3A^RNAi.UAS.WIZ

Species

D. melanogaster

Name

FlyBase ID

FBal0247473

Feature type

allele

Associated gene

Dmel\Sin3A

Associated Insertion(s)

Carried in Construct

P{UAS-Sin3A.RNAi.WIZ}

Also Known As

UAS-SIN3^RNAi

Key Links

Transgenic product class

RNAi_reagent

(Sharma et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Sharma et al., 2008)

Mutagen

in vitro construct

(Sharma et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-Sin3A.RNAi.WIZ}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat of a 730bp fragment from the third exon of Sin3A.

(Sharma et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Sin3A

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased occurrence of cell division | larval stage, with Scer\GAL4^Bx-MS1096-KE

(Swaminathan and Pile, 2010)

hypoplasia, with Scer\GAL4^Ser.PGF

(Swaminathan and Pile, 2010)

hypoplasia | somatic clone, with Scer\GAL4^Act5C.PP

(Swaminathan and Pile, 2010)

lethal | embryonic stage, with Scer\GAL4^Act5C.PI

(Sharma et al., 2008)

lethal | embryonic stage, with Scer\GAL4^αTub84B.PL

(Sharma et al., 2008)

lethal | larval stage, with Scer\GAL4^Act5C.PI

(Sharma et al., 2008)

lethal | larval stage, with Scer\GAL4^αTub84B.PL

(Sharma et al., 2008)

lethal - all die before end of larval stage | heat sensitive, with Scer\GAL4^hs.PB

(Sharma et al., 2008)

some die during larval stage | heat sensitive, with Scer\GAL4^hs.PB

(Sharma et al., 2008)

visible, with Scer\GAL4^A9

(Swaminathan and Pile, 2010)

visible, with Scer\GAL4^Bx-MS1096-KE

(Swaminathan and Pile, 2010)

visible, with Scer\GAL4^en-e16E

(Swaminathan and Pile, 2010)

visible, with Scer\GAL4^{rn-GAL4-DeltaS}

(Swaminathan and Pile, 2010)

visible, with Scer\GAL4^Ser.PGF

(Swaminathan and Pile, 2010)

visible, with Scer\GAL4^vg.M.Exel

(Swaminathan and Pile, 2010)

Phenotype Manifest In

wing blade, with Scer\GAL4^A9

(Swaminathan and Pile, 2010)

wing blade, with Scer\GAL4^Bx-MS1096-KE

(Swaminathan and Pile, 2010)

wing blade, with Scer\GAL4^en-e16E

(Swaminathan and Pile, 2010)

wing blade, with Scer\GAL4^{rn-GAL4-DeltaS}

(Swaminathan and Pile, 2010)

wing blade, with Scer\GAL4^Ser.PGF

(Swaminathan and Pile, 2010)

wing blade, with Scer\GAL4^vg.M.Exel

(Swaminathan and Pile, 2010)

wing disc, with Scer\GAL4^Bx-MS1096-KE

(Swaminathan and Pile, 2010)

wing disc, with Scer\GAL4^Ser.PGF

(Swaminathan and Pile, 2010)

wing disc | somatic clone, with Scer\GAL4^Act5C.PP

(Swaminathan and Pile, 2010)

wing vein, with Scer\GAL4^Ser.PGF

(Swaminathan and Pile, 2010)

wing vein L5, with Scer\GAL4^en-e16E

(Swaminathan and Pile, 2010)

wing vein L5, with Scer\GAL4^Ser.PGF

(Swaminathan and Pile, 2010)

Detailed Description

Statement

Reference

Expression of Sin3A^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^Ser.PGF results in a curly wing phenotype.

Expression of Sin3A^{dsRNA.Scer\UAS.WIZ} under the control of any one of Scer\GAL4^A9, Scer\GAL4^vg.M.Exel, Scer\GAL4^{rn-GAL4-DeltaS} or Scer\GAL4^Bx-MS1096-KE results in a curly wing phenotype.

Expression of Sin3A^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^en-e16E results in incomplete L5 wing vein. Curly wing is observed only in the posterior compartment when Sin3A^{dsRNA.Scer\UAS.WIZ} is expressed under the control of Scer\GAL4^en-e16E.

Expression of Scer\GAL4^Ser.PGF>Sin3A^{dsRNA.Scer\UAS.WIZ} results in smaller wing imaginal discs and smaller adult wings compared with wild-type. In flies carrying Scer\GAL4^Ser.PGF>Sin3A^{dsRNA.Scer\UAS.WIZ} wing discs are 5% smaller than wild-type. Adult wings expressing Sin3A^{dsRNA.Scer\UAS.WIZ} are reduced in size relative to control wings due to reduced cell number.

About 3% of the Scer\GAL4^Ser.PGF>Sin3A^{dsRNA.Scer\UAS.WIZ} wings show venation defects including thinner veins and incomplete L5 vein.

Somatic clones of cells expressing Scer\GAL4^Act5C.PP>Sin3A^{dsRNA.Scer\UAS.WIZ} in the wing disc are fewer in number and smaller in size than clones in control discs. Scer\GAL4^Act5C.PP>Sin3A^{dsRNA.Scer\UAS.WIZ} clones are not found in discs when clones are induced early in development.

Expression of Sin3A^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^Bx-MS1096-KE leads to a reduction in the number of mitotic cells present in wing imaginal dis tissue. Compared with controls, the number of cells in S phase is not altered following Scer\GAL4^Bx-MS1096-KE>Sin3A^{dsRNA.Scer\UAS.WIZ} expression.

(Swaminathan and Pile, 2010)

Temperature shift experiments in which expression of Sin3A^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^hs.PB is induced using heat shock show that expression of Sin3A^{dsRNA.Scer\UAS.WIZ} during the first or second larval instar results in lethality before the wandering third instar, while expression of Sin3A^{dsRNA.Scer\UAS.WIZ} in wandering third instar larvae or pupae has no effect on viability. The dead larvae have no gross phenotypic abnormalities.

(Sharma et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^Ser.PGF, Sin3A^RNAi.UAS.WIZ has visible phenotype, enhanceable by cdc2[+]/Cdk1^c03495

(Swaminathan and Pile, 2010)

NOT Enhanced by

Statement

Reference

Scer\GAL4^Ser.PGF, Sin3A^RNAi.UAS.WIZ has visible phenotype, non-enhanceable by Df(3L)GN24/+

(Swaminathan and Pile, 2010)

Suppressed by

Statement

Reference

Scer\GAL4^Ser.PGF, Sin3A^RNAi.UAS.WIZ has visible phenotype, suppressible | partially by stg^UAS.cNa/Scer\GAL4^Ser.PGF

(Swaminathan and Pile, 2010)

Scer\GAL4^Ser.PGF, Sin3A^RNAi.UAS.WIZ has hypoplasia phenotype, suppressible by stg^UAS.cNa/Scer\GAL4^Ser.PGF

(Swaminathan and Pile, 2010)

Scer\GAL4^Ser.PGF, Sin3A^RNAi.UAS.WIZ has visible phenotype, suppressible | partially by Cdk1^UAS.Tag:MYC, Scer\GAL4^Ser.PGF

(Swaminathan and Pile, 2010)

Scer\GAL4^Ser.PGF, Sin3A^RNAi.UAS.WIZ has visible phenotype, suppressible | partially by Df(3L)iro-2/+

(Swaminathan and Pile, 2010)

Scer\GAL4^Ser.PGF, Sin3A^RNAi.UAS.WIZ has visible phenotype, suppressible | partially by Gcn5^C137Y/Gcn5[+]

(Swaminathan and Pile, 2010)

Scer\GAL4^Ser.PGF, Sin3A^RNAi.UAS.WIZ has visible phenotype, suppressible | partially by Gcn5[+]/Gcn5^ΔT280-F285

(Swaminathan and Pile, 2010)

NOT suppressed by

Statement

Reference

Scer\GAL4^Ser.PGF, Sin3A^RNAi.UAS.WIZ has visible phenotype, non-suppressible by Gcn5^Q186st/Gcn5[+]

(Swaminathan and Pile, 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^Ser.PGF, Sin3A^RNAi.UAS.WIZ has wing blade phenotype, enhanceable by cdc2[+]/Cdk1^c03495

(Swaminathan and Pile, 2010)

NOT Enhanced by

Statement

Reference

Scer\GAL4^Ser.PGF, Sin3A^RNAi.UAS.WIZ has wing blade phenotype, non-enhanceable by Df(3L)GN24/+

(Swaminathan and Pile, 2010)

Suppressed by

Statement

Reference

Scer\GAL4^Ser.PGF, Sin3A^RNAi.UAS.WIZ has wing blade phenotype, suppressible | partially by Df(3L)iro-2/+

(Swaminathan and Pile, 2010)

Scer\GAL4^Ser.PGF, Sin3A^RNAi.UAS.WIZ has wing blade phenotype, suppressible | partially by Gcn5^C137Y/Gcn5[+]

(Swaminathan and Pile, 2010)

Scer\GAL4^Ser.PGF, Sin3A^RNAi.UAS.WIZ has wing blade phenotype, suppressible | partially by Gcn5[+]/Gcn5^ΔT280-F285

(Swaminathan and Pile, 2010)

Scer\GAL4^Ser.PGF, Sin3A^RNAi.UAS.WIZ has wing blade phenotype, suppressible | partially by stg^UAS.cNa/Scer\GAL4^Ser.PGF

(Swaminathan and Pile, 2010)

Scer\GAL4^Ser.PGF, Sin3A^RNAi.UAS.WIZ has wing blade phenotype, suppressible | partially by Cdk1^UAS.Tag:MYC, Scer\GAL4^Ser.PGF

(Swaminathan and Pile, 2010)

NOT suppressed by

Statement

Reference

Scer\GAL4^Ser.PGF, Sin3A^RNAi.UAS.WIZ has wing blade phenotype, non-suppressible by Gcn5^Q186st/Gcn5[+]

(Swaminathan and Pile, 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Induction of Sin3A^{dsRNA.Scer\UAS.WIZ} using Scer\GAL4^Ser.PGF in a heterozygous cdc2^c03495 genetic background results in a more aberrant wing phenotype than that of the Scer\GAL4^Ser.PGF>Sin3A^{dsRNA.Scer\UAS.WIZ} expression alone.

Overexpression of cdc2^{Scer\UAS.T:Hsap\MYC} partially suppresses the curly wing phenotype in Scer\GAL4^Ser.PGF>Sin3A^{dsRNA.Scer\UAS.WIZ} flies.

Co-expression of stg^Scer\UAS.cNa results in the partial suppression of the curly wing phenotype induced by Scer\GAL4^Ser.PGF>Sin3A^{dsRNA.Scer\UAS.WIZ} and restoration of the size of the adult wing blade to that of control wings as well as restoration of cell number.

Gcn5^ΔT280-F285/+ partially suppresses the wing phenotype induced by Scer\GAL4^Ser.PGF>Sin3A^{dsRNA.Scer\UAS.WIZ}.

Gcn5^C137T/+ partially suppresses the wing phenotype induced by Scer\GAL4^Ser.PGF>Sin3A^{dsRNA.Scer\UAS.WIZ}.

Gcn5^Q186st/+ fails to suppress the wing phenotype induced by Scer\GAL4^Ser.PGF>Sin3A^{dsRNA.Scer\UAS.WIZ}.

Df(3L)GN24/+ does not enhance the Scer\GAL4^Ser.PGF>Sin3A^{dsRNA.Scer\UAS.WIZ} induced wing phenotype.

Df(3L)iro-2/+ partially suppressed the curly wing phenotype induced by Scer\GAL4^Ser.PGF>Sin3A^{dsRNA.Scer\UAS.WIZ}.

(Swaminathan and Pile, 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Sin3A^RNAi.UAS.WIZ is partially rescued by Scer\GAL4^αTub84B.PL/Sin3A^187.UAS

(Sharma et al., 2008)

Sin3A^RNAi.UAS.WIZ is partially rescued by Sin3A^220.UAS/Scer\GAL4^αTub84B.PL

(Sharma et al., 2008)

Comments

Co-expression of Sin3A^187.Scer\UAS partially rescues the lethality caused by expression of Sin3A^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^αTub84B.PL.

Co-expression of Sin3A^220.Scer\UAS partially rescues the lethality caused by expression of Sin3A^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^αTub84B.PL.

(Sharma et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Sin3A^RNAi.UAS.WIZ

Sin3A^{dsRNA.Scer\UAS.WIZ}

Sin3A^{dsRNA.UAS.WIZ}

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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