FlyBase Allele Report: Dmel\Osbp[UAS.cMa]

General Information

Symbol

Dmel\Osbp^UAS.cMa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Ma

FlyBase ID

FBal0248438

Feature type

allele

Associated gene

Dmel\Osbp

Associated Insertion(s)

Carried in Construct

P{UAS-Osbp.m}

Also Known As

UAS-Osbp

Key Links

Transgenic product class

wild_type

(Ma et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ma et al., 2010)

Mutagen

in vitro construct

(Ma et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-Osbp.m}

Cytology

Description

UASt regulatory sequences drive expression of full-length Osbp.

(Ma et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Osbp

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^Act5C.PU

(Ma et al., 2010)

Phenotype Manifest In

adult cuticle, with Scer\GAL4^Act5C.PU

(Ma et al., 2010)

adult thorax, with Scer\GAL4^Act5C.PU

(Ma et al., 2010)

adult thorax, with Scer\GAL4^CCAP.PP

(Ma et al., 2010)

Golgi apparatus, with Scer\GAL4^CCAP.PP

(Ma et al., 2012)

wing, with Scer\GAL4^Act5C.PU

(Ma et al., 2010)

wing, with Scer\GAL4^CCAP.PP

(Ma et al., 2012, Ma et al., 2010)

Detailed Description

Statement

Reference

Scer\GAL4^Ccap.PP-mediated expression of Osbp^Scer\UAS.cMa does not affect outgrowth of Scer\GAL4^Ccap.PP-expressing axons.

Scer\GAL4^Ccap.PP-mediated expression of Osbp^Scer\UAS.cMa alters the morphology of the Golgi apparatus in Scer\GAL4^Ccap.PP-expressing neurons.

Flies expressing Osbp^Scer\UAS.cMa under the control of Scer\GAL4^Ccap.PP have unexpanded wings with complete penetrance.

(Ma et al., 2012)

Flies expressing Osbp^Scer\UAS.cMa under the control of Scer\GAL4^Act5C.PU do not expand their wings and thorax, and show abnormally tanned cuticles.

Flies expressing Osbp^Scer\UAS.cMa under the control of Scer\GAL4^Ccap.PP display defects in wing and thorax expansion.

(Ma et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Osbp^UAS.cMa/Scer\GAL4^Hsp83.PA is a suppressor of semi-sterile phenotype of fan^{RNAi.βTub85D}

(Ma et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Df(3L)Exel6112/+

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Df(3L)Exel6120/+

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Df(3L)Exel6136/+

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Df(3R)Exel6167/+

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Df(3R)Exel6211/+

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Df(3R)Exel6212/+

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Df(3R)Exel6215/+

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by +/Df(3R)Exel6143

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Df(3R)Exel6170/+

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Df(3R)Exel6184/+

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Df(3R)Exel6193/+

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Df(3R)Exel9013/+

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Df(3R)Exel6196/+

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by +/Df(3R)Exel6200

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Df(3R)Exel6208/+

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Df(3R)Exel6209/+

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by +/Df(3R)Exel6213

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Df(3R)Exel6216/+

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible by Df(2R)ED3181/+

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Rab18^{A64L.UASp.YFP}, Scer\GAL4^CCAP.PP

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Rab10^{Q68L.UASp.YFP}, Scer\GAL4^CCAP.PP

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Rab11^{Q70L.UASp.YFP}, Scer\GAL4^CCAP.PP

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Rab26^{Q250L.UAS.YFP}, Scer\GAL4^CCAP.PP

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Rab35^{Q67L.UASp.YFP}, Scer\GAL4^CCAP.PP

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Rab40^{Q75L.UASp.YFP}, Scer\GAL4^CCAP.PP

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by RabX2^{D66L.UASp.YFP}, Scer\GAL4^CCAP.PP

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Rab7^{T22N.UASp.YFP}, Scer\GAL4^CCAP.PP

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Rab19^T35N.UAS.YFP, Scer\GAL4^CCAP.PP

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible by Df(2L)Exel8022/+

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible by Df(2L)Exel7042/+

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible by Df(2R)Exel6066/+

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by CG33298^44-5/CG33298[+]

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has Golgi apparatus phenotype, suppressible by CG33298^44-5/CG33298[+]

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible by Df(3R)Exel7283/+

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Df(2L)Exel6022/+

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Df(3L)Exel6087/+

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Df(3L)Exel6092/+

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible | partially by Df(3L)Exel6102/+

(Ma et al., 2012)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible by fan^UAS.Tag:HA, Scer\GAL4^CCAP.PP

(Ma et al., 2010)

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, suppressible by Vap33^UAS.cPa, Scer\GAL4^CCAP.PP

(Ma et al., 2010)

NOT suppressed by

Statement

Reference

Osbp^UAS.cMa, Scer\GAL4^CCAP.PP has wing phenotype, non-suppressible by Gdi[+]/Gdi^N7-3

(Ma et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Df(2R)Exel6066/+ and Df(3R)Exel7283/+ fully suppress the uninflated wing phenotype of Scer\GAL4^Ccap.PP, Osbp^Scer\UAS.cMa flies.

Df(2L)Exel6022/+, Df(3L)Exel6087/+, Df(3L)Exel6092/+, Df(3L)Exel6102/+, Df(3L)Exel6112/+, Df(3L)Exel6120/+, Df(3L)Exel6136/+, Df(3R)Exel6167/+, Df(3R)Exel6211/+, Df(3R)Exel6212/+ and Df(3R)Exel6215/+ strongly suppress the uninflated wing phenotype of Scer\GAL4^Ccap.PP, Osbp^Scer\UAS.cMa flies.

Df(3R)Exel6143/+, Df(3R)Exel6170/+, Df(3R)Exel6184/+, Df(3R)Exel6193/+, Df(3R)Exel9013/+, Df(3R)Exel6196/+ Df(3R)Exel6200/+, Df(3R)Exel6208/+, Df(3R)Exel6209/+, Df(3R)Exel6213/+ and Df(3R)Exel6216/+ weakly suppress the uninflated wing phenotype of Scer\GAL4^Ccap.PP, Osbp^Scer\UAS.cMa flies.

Co-expression of Rab18^{A64L.Scer\UAS.P\T.T:Avic\GFP-YFP} strongly suppresses the uninflated wing phenotype of Scer\GAL4^Ccap.PP, Osbp^Scer\UAS.cMa flies.

Co-expression of Rab10^{Q68L.Scer\UAS.P\T.T:Avic\GFP-YFP}, Rab11^{Q70L.Scer\UAS.P\T.T:Avic\GFP-YFP}, Rab26^{Q250L.Scer\UAS.T:Avic\GFP-YFP}, Rab35^{Q67L.Scer\UAS.P\T.T:Avic\GFP-YFP}, Rab40^{Q75L.Scer\UAS.P\T.T:Avic\GFP-YFP}, RabX2^{D66L.Scer\UAS.P\T.T:Avic\GFP-YFP}, Rab7^{T22N.Scer\UAS.P\T.T:Avic\GFP-YFP} or Rab19^{T35N.Scer\UAS.T:Avic\GFP-YFP} weakly suppresses the uninflated wing phenotype of Scer\GAL4^Ccap.PP, Osbp^Scer\UAS.cMa flies.

CG33298^44-5/+ suppresses while Gdi^N7-3/+ fails to suppress the uninflated wing phenotype of Scer\GAL4^Ccap.PP, Osbp^Scer\UAS.cMa flies.

CG33298^44-5/+ partially suppresses the Golgi morphology phenotype caused by Scer\GAL4^Ccap.PP-mediated expression of Osbp^Scer\UAS.cMa.

(Ma et al., 2012)

Expression of fan^{Scer\UAS.T:Ivir\HA1} in flies expressing Osbp^Scer\UAS.cMa under the control of Scer\GAL4^Ccap.PP suppresses the unexpanded wing phenotype.

Expression of Vap-33-1^Scer\UAS.cPa in flies expressing Osbp^Scer\UAS.cMa under the control of Scer\GAL4^Ccap.PP suppresses the unexpanded wing phenotype.

Expression of Osbp^Scer\UAS.cMa under the control of Scer\GAL4^Hsp83.PA significantly rescues the reduced fertility observed in fan^{dsRNA.βTub85D} animals.

(Ma et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Osbp^UAS.cMa/Scer\GAL4^{VP16.nanos.UTR} rescues Osbp¹

(Ma et al., 2010)

Comments

Expression of Osbp^Scer\UAS.cMa under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} significantly restores the fertility of Osbp¹ animals.

(Ma et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

57346

y¹ w^*; P{UAS-GFP.U}2; P{UAS-Osbp.m}3/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Osbp^Scer\UAS.cMa

Osbp^UAS.cMa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Ma

Secondary FlyBase IDs

References (4)

Report Sections

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