FlyBase Allele Report: Dmel\Syn1[NIG.7152R]

General Information

Symbol

Dmel\Syn1^NIG.7152R

Species

D. melanogaster

Name

FlyBase ID

FBal0248513

Feature type

allele

Associated gene

Dmel\Syn1

Associated Insertion(s)

Carried in Construct

P{NIG.7152R}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Nagai et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Nagai et al., 2010)

Mutagen

in vitro construct

(Nagai et al., 2010)

Progenitor genotype

Carried in construct

P{NIG.7152R}

Cytology

Description

UASt regulatory sequences drive expression of a Syn1 RNAi construct.

(Nagai et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-7152R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal - majority die, with Scer\GAL4^Act5C.PU

(Nagai et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

The viability of animals expressing Syn1^NIG.7152R under the control of Scer\GAL4^Act5C.PU is reduced to 62.3%.

Flies expressing Syn1^NIG.7152R under the control of Scer\GAL4^Act5C.PU show no clear change in locomotion ability in a climbing assay compared to wild-type controls.

Larvae expressing Syn1^NIG.7152R under the control of Scer\GAL4^Act5C.PU do not have any apparent changes in neuromuscular junction morphology.

(Nagai et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^Act5C.PU, Syn1^NIG.7152R has partially lethal - majority die phenotype, enhanceable by Syn2³⁷

(Nagai et al., 2010)

Enhancer of

Statement

Reference

Scer\GAL4^Act5C.PU/Syn1^NIG.7152R is an enhancer of abnormal locomotor behavior | adult stage phenotype of Syn2³⁷

(Nagai et al., 2010)

Other

Statement

Reference

Nsf2^EQ.UAS, Scer\GAL4^elav.PLu, Syn1^NIG.7152R has abnormal neuroanatomy | third instar larval stage phenotype

(Nagai et al., 2010)

Nsf2^EQ.UAS, Scer\GAL4^elav.PLu, Syn1^NIG.7152R has abnormal locomotor behavior | third instar larval stage phenotype

(Nagai et al., 2010)

Nsf2^EQ.UAS, Scer\GAL4^elav.PLu, Syn1^NIG.7152R, Syn2³⁷ has abnormal neuroanatomy | third instar larval stage phenotype

(Nagai et al., 2010)

Nsf2^EQ.UAS, Scer\GAL4^elav.PLu, Syn1^NIG.7152R, Syn2³⁷ has abnormal locomotor behavior | third instar larval stage phenotype

(Nagai et al., 2010)

Scer\GAL4^Act5C.PU, Syn1^NIG.7152R, Syn2³⁷ has short lived phenotype

(Nagai et al., 2010)

Phenotype Manifest In

Other

Statement

Reference

Nsf2^EQ.UAS, Scer\GAL4^elav.PLu, Syn1^NIG.7152R has embryonic/larval neuromuscular junction phenotype

(Nagai et al., 2010)

Nsf2^EQ.UAS, Scer\GAL4^elav.PLu, Syn1^NIG.7152R, Syn2³⁷ has embryonic/larval neuromuscular junction phenotype

(Nagai et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The viability of animals expressing Syn1^NIG.7152R under the control of Scer\GAL4^Act5C.PU in a Syn2³⁷ background is reduced to 13.8%. The double mutant flies pupate and develop into pharate adults that do not show any apparent morphological defects, but the majority do not hatch. Some flies manage to protrude their heads out of the pupal case, but are not able to eclose fully and eventually die.

Flies expressing Syn1^NIG.7152R under the control of Scer\GAL4^Act5C.PU in a Syn2³⁷ background have a median lifespan of 27 days, significantly shorter than that of wild-type controls.

Flies expressing Syn1^NIG.7152R under the control of Scer\GAL4^Act5C.PU in a Syn2³⁷ background have a strongly reduced mobility in a climbing assay compared to wild-type controls, especially in older animals.

Larvae expressing Syn1^NIG.7152R under the control of Scer\GAL4^Act5C.PU in a Syn2³⁷ background do not have any apparent changes in neuromuscular junction morphology.

Larvae co-expressing Nsf2^EQ.Scer\UAS and Syn1^NIG.7152R under the control of Scer\GAL4^elav.PLu show only a weak increase in total synaptic branch length at the neuromuscular junction. These larvae show a slightly reduced frequency of peristaltic waves compared to wild type.

Larvae co-expressing Nsf2^EQ.Scer\UAS and Syn1^NIG.7152R under the control of Scer\GAL4^elav.PLu in a Syn2³⁷ background show a very dramatic increase in total synaptic branch length at the neuromuscular junction. These larvae show a reduced frequency of peristaltic waves compared to wild type.

(Nagai et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Syn1^NIG.7152R

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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