UAS regulatory sequences drive expression of wild-type Ir84a with the endogenous signal sequence replaced with Tag:SS(hCALR) and fused to EGFP.
Neurons expressing Ir84aScer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP driven by Scer\GAL4Or22a.7.717 do not respond to phenylacetaldehyde above basal solvent-evoked activity.
Neurons co-expressing Ir84aScer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP and Ir8aScer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP under the control of Scer\GAL4Or22a.7.717 respond to phenylacetaldehyde in a concentration-dependent manner.
Neurons co-expressing Ir84aScer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP and Ir8aScer\UAS.T:Hsap\CALR,T:Disc\RFP-mCherry under the control of Scer\GAL4Or22a.7.717 respond to phenylacetaldehyde in a concentration-dependent manner.
Neurons co-expressing Ir84aScer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP and Ir8aScer\UAS.cAa under the control of Scer\GAL4Or22a.7.717 respond to phenylacetaldehyde in a concentration-dependent manner.
Expression of Ir84aScer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP using Scer\GAL4Ir84a-GAL4 restores the response to phenylacetic acid and phenylacetaldehyde in Ir84aGAL4 homozygotes.