FlyBase Allele Report: Dmel\N[NIG.3936R]

General Information

Symbol

Dmel\N^NIG.3936R

Species

D. melanogaster

Name

FlyBase ID

FBal0256935

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

P{NIG.3936R}

Also Known As

N RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Wang et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Wang et al., 2011)

Mutagen

in vitro construct

(Wang et al., 2011)

Progenitor genotype

Carried in construct

P{NIG.3936R}

Cytology

Description

UASt regulatory sequences drive expression of a N RNAi construct.

(Wang et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-3936R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^c768

(Wang et al., 2011)

abnormal neuroanatomy | larval stage, with Scer\GAL4^C855a

(Wang et al., 2011)

neoplasia | adult stage | somatic clone, with Scer\GAL4^unspecified

(Lin et al., 2013)

visible, with Scer\GAL4^MD-638

(Molnar et al., 2011)

Phenotype Manifest In

adult posterior midgut epithelium | somatic clone, with Scer\GAL4^unspecified

(Lin et al., 2013)

lamina anlage, with Scer\GAL4^c768

(Wang et al., 2011)

lamina anlage, with Scer\GAL4^C855a

(Wang et al., 2011)

larval outer optic anlage, with Scer\GAL4^c768

(Wang et al., 2011)

larval outer optic anlage, with Scer\GAL4^C855a

(Wang et al., 2011)

medulla anlage, with Scer\GAL4^c768

(Wang et al., 2011)

medulla anlage, with Scer\GAL4^C855a

(Wang et al., 2011)

wing margin, with Scer\GAL4^MD-638

(Molnar et al., 2011)

wing vein, with Scer\GAL4^MD-638

(Molnar et al., 2011)

Detailed Description

Statement

Reference

Expression of N^NIG.3936R in randomly induced clones, in combination with a Gal80[ts] transgene to restrict expression to the two weeks of adulthood prior to analysis, leads to the formation of intestinal stem cell-like tumors in the posterior midgut.

(Lin et al., 2013)

Expression of N^NIG.3936R under the control of Scer\GAL4^MD-638 causes the thickening of the wing veins and the elimination of the wing margin.

(Molnar et al., 2011)

Expression of N^NIG.3936R under the control of either Scer\GAL4^C855a or Scer\GAL4^c768 results in the disappearance of neuroepithelial cells in the outer proliferation center of the optic lobe during early to mid-third larval instar, premature formation of medulla neuroblasts and premature neurogenesis in the medulla. Late third instar larvae show complete loss of the lamina and have a smaller than normal medulla cortex. The eye imaginal disc, central brain and ventral nerve cord appear normal in these larvae.

Expression of N^NIG.3936R under the control of either Scer\GAL4¹⁴⁰⁷ or Scer\GAL4^elav-C155 does not cause any defects in the development of the optic lobe, central brain and ventral nerve cord.

Expression of N^NIG.3936R under the control of Scer\GAL4¹⁴⁰⁷ in the larval optic lobe does not affect medulla neuroblast formation or their self-renewing ability.

(Wang et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

N^NIG.3936R, Scer\GAL4^MD-638 has visible phenotype, enhanceable by krz^RNAi.UAS, Scer\GAL4^MD-638

(Molnar et al., 2011)

Suppressed by

Statement

Reference

N^NIG.3936R, Scer\GAL4^unspecified has neoplasia | somatic clone | adult stage phenotype, suppressible | partially | somatic clone by mys^unspecified

(Lin et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

N^NIG.3936R, Scer\GAL4^MD-638 has wing vein phenotype, enhanceable by krz^RNAi.UAS, Scer\GAL4^MD-638

(Molnar et al., 2011)

N^NIG.3936R, Scer\GAL4^MD-638 has wing margin phenotype, enhanceable by krz^RNAi.UAS, Scer\GAL4^MD-638

(Molnar et al., 2011)

Suppressed by

Statement

Reference

N^NIG.3936R, Scer\GAL4^unspecified has adult posterior midgut epithelium | somatic clone phenotype, suppressible | partially | somatic clone by mys^unspecified

(Lin et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The wing vein and wing margin phenotypes resulting from the expression of N^NIG.3936R under the control of Scer\GAL4^MD-638 are augmented by the co-expression of krz^{dsRNA.Scer\UAS}.

(Molnar et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

N^NIG.3936R-2

(Casso et al., 2011)

N^NIG.3936R

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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