FB2024_03 , released June 25, 2024
Allele: Dmel\Pink1K337R.D479A.D501A.UAS.Tag:HA
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General Information
Symbol
Dmel\Pink1K337R.D479A.D501A.UAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0258534
Feature type
allele
Associated gene
Dmel\Pink1
Associated Insertion(s)
Carried in Construct
P{UAS-Pink1.HA.KD}
Key Links
Transgenic product class
missense_variant
(Kim et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Kim et al., 2008)
Mutagen
in vitro construct
(Kim et al., 2008)
Progenitor genotype
Carried in construct
P{UAS-Pink1.HA.KD}
Cytology
Description

UAS regulatory sequences drive expression of a kinase inactive mutant (K337R, D479A, and D501A) of Pink1 tagged with a Tag:HA epitope.

(Kim et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Pink1
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing Pink1K337R.D479A.D501A.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF exhibit a rough eye phenotype and disarrayed ommatidia.

      (Song et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      NOT suppressed by
      Phenotype Manifest In
      NOT Enhanced by
      NOT suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of parkScer\UAS.T:Hsap\MYC has no effect on the rough eye phenotype seen when Pink1K337R.D479A.D501A.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PF.

      (Song et al., 2013)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      Pink1K337R.D479A.D501A.UAS.Tag:HA/Scer\GAL4hs.PB fails to rescue Pink1B9

      (Song et al., 2013)

      Pink1K337R.D479A.D501A.UAS.Tag:HA fails to rescue Pink1B9

      (Kim et al., 2008)
      Comments

      Expressing Pink1K337R.D479A.D501A.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4hs.PB fails to rescue the crushed thorax and downturned wing phenotypes seen Pink1B9 mutant flies at 3 days post-eclosion. The mitochondrial defects, reduction in ATP levels and flightlessness are also not rescued. Expression of Pink1K337R.D479A.D501A.Scer\UAS.T:Ivir\HA1 fails to rescue phenotypes seen in 45 day old Pink1B9 mutant flies, with both the downturned wing and dopaminergic neuron loss still seen in these flies.

      (Song et al., 2013)

      Expression of Pink1K337R.D479A.D501A.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4hs.PB fails to rescue the apparent behavioural phenotypes of Pink1B9 mutants. Impaired mitochondria in the indirect flight muscle of Pink1B9 mutants are not recovered by Pink1K337R.D479A.D501A.Scer\UAS.T:Ivir\HA1 expression under the control of Scer\GAL4hs.PB.

      (Kim et al., 2008)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Pink1K337R.D479A.D501A.Scer\UAS.T:Ivir\HA1
      Pink1K337R.D479A.D501A.UAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (2)