FlyBase Allele Report: Dmel\E(Pc)[GL00169]

General Information

Symbol

Dmel\E(Pc)^GL00169

Species

D. melanogaster

Name

FlyBase ID

FBal0262643

Feature type

allele

Associated gene

Dmel\E(Pc)

Associated Insertion(s)

Carried in Construct

P{TRiP.GL00169}

Also Known As

UAS-E(Pc) shRNA

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.GL00169}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-GL00169

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | clone of cells | spermatogenesis, with Scer\GAL4^Act5C.PU

(Feng et al., 2017)

increased cell number | spermatogenesis, with Scer\GAL4^NP1624

(Feng et al., 2017)

neoplasia | spermatogenesis, with Scer\GAL4^NP1624

(Feng et al., 2017)

visible | adult stage, with Scer\GAL4^ey.PH

(Dietz et al., 2015)

visible | adult stage, with Scer\GAL4^GMR.PF

(Dietz et al., 2015)

Phenotype Manifest In

cyst progenitor cell | clone of cells | increased number, with Scer\GAL4^Act5C.PU

(Feng et al., 2017)

cyst progenitor cell | increased number, with Scer\GAL4^NP1624

(Feng et al., 2017)

eye, with Scer\GAL4^ey.PH

(Dietz et al., 2015)

eye, with Scer\GAL4^GMR.PF

(Dietz et al., 2015)

male germline cell | increased number, with Scer\GAL4^NP1624

(Feng et al., 2017)

male germline stem cell | increased number, with Scer\GAL4^NP1624

(Feng et al., 2017)

spermatogonial cyst, with Scer\GAL4^NP1624

(Feng et al., 2017)

testis, with Scer\GAL4^NP1624

(Feng et al., 2017)

Detailed Description

Statement

Reference

The expression of E(Pc)^JF03101 under the control of E(Pc)^GL00169 leads to testes showing increased numbers of cyst stem cells (Yan-, Tj-, or Zfh-1-positive cells), associated with cyst stem cell differentiation defects, as illustrated by a high proportion of cells abnormally co-expressing Zfh-1 (marker of cyst stem cells/early cyst cells) and Eya (marker of later stage cyst cells).

Unlike control testes, there is frequent expansion of early stage germ cells from the apical tip region of the testes; some resemble stem cells or GB-tumor with round spectrosome structures, while some other resemble spermatogonial tumors with more than 16 cells; cells within one cyst show both dotted spectrosome and branched fusome structures, as they undergo asynchronous cell cycle; some cells also express early cyst cell markers (Yan and Zfh-1).

Similarly, clonal expression under the control of Scer\GAL4^Act5C.PU leads to increased number of cells with ectopic expression of Zfh-1.

(Feng et al., 2017)

Expression of E(Pc)^GL00169 under the control of either Scer\GAL4^ey.PH or Scer\GAL4^GMR.PF results in rough eye phenotype in adults.

(Dietz et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference