FB2024_03 , released June 25, 2024
Allele: Dmel\E(spl)m8-HLHS159D.123-179.CtD.UAS
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General Information
Symbol
Dmel\E(spl)m8-HLHS159D.123-179.CtD.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0264412
Feature type
allele
Associated gene
Dmel\E(spl)m8-HLH
Associated Insertion(s)
Carried in Construct
P{UAS-E(spl)m8-HLH.SD.123-179}
Key Links
Transgenic product class
inframe_deletion
(Kahali et al., 2010)
missense_variant
(Kahali et al., 2010)
polypeptide_post_translational_processing_variant
(Kahali et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Kahali et al., 2010)
missense_variant
(Kahali et al., 2010)
polypeptide_post_translational_processing_variant
(Kahali et al., 2010)
Mutagen
in vitro construct
(Kahali et al., 2010)
Progenitor genotype
Carried in construct
P{UAS-E(spl)m8-HLH.SD.123-179}
Cytology
Description

Amino acid replacement: S159D.

(Kahali et al., 2010)

UASt regulatory sequences drive expression of the C-terminal 123-179 residues of E(spl)m8-HLH in which the CK2 site has been mutated to a phosphomimetic residue (changes is S159D). This region should thus lack autoinhibitory activity.

(Kahali et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
E(spl)m8-HLH
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of E(spl)123-179.CtD.Scer\UAS in the proneural clusters, under the control of Scer\GAL4455.2 elicits ectopic macrochaeta on the scutellum of 31-54% of flies, with an average penetrance of approximately 41%.

      (Kahali et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      Scer\GAL4455.2/E(spl)m8-HLHS159D.123-179.CtD.UAS fails to rescue E(spl)m8-HLHUAS.cKa

      (Kahali et al., 2010)

      E(spl)m8-HLHS159D.123-179.CtD.UAS/Scer\GAL4sca-109-68 fails to rescue E(spl)m8-HLHSD.UAS

      (Kahali et al., 2010)
      Comments

      Co-expression of E(spl)S159D.123-179.CtD.Scer\UAS under the control of Scer\GAL4sca-109-68 does not rescue the reduced eye found upon expression of E(spl)SD.Scer\UAS nor the aberrant ommatidial size or patterning.

      Co-expression of E(spl)S159D.123-179.CtD.Scer\UAS elicits facet numbers (~650-670) that are indistinguishable from those in Scer\GAL4sca-109-68 E(spl)SD.Scer\UAS flies.

      Co-expression of E(spl)S159D.123-179.CtD.Scer\UAS does not restore the scutellar macrochaeta lost in a Scer\GAL4455.2>E(spl)Scer\UAS.cKa background.

      (Kahali et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      E(spl)S159D.123-179.CtD.Scer\UAS
      E(spl)m8-HLHS159D.123-179.CtD.Scer\UAS
      E(spl)m8-HLHS159D.123-179.CtD.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)