FlyBase Allele Report: Dmel\mir-8[SC1]

General Information

Symbol

Dmel\mir-8^SC1

Species

D. melanogaster

Name

FlyBase ID

FBal0265087

Feature type

allele

Associated gene

Dmel\mir-8

Associated Insertion(s)

P{GS-un}mir-8^SC1

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{GS-un}mir-8^SC1

Cytology

Description

Insertion 1.1kb from the mir-8 gene.

(Vallejo et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by lola^GS88A8, psq^GS88A8

(Vallejo et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | first instar larval stage, with Scer\GAL4^ey.PH

(Vallejo et al., 2011)

increased cell death | third instar larval stage, with Scer\GAL4^ey.PH

(Vallejo et al., 2011)

visible, with Scer\GAL4^Bx-MS1096

(Vallejo et al., 2011)

visible, with Scer\GAL4^ey.PH

(Vallejo et al., 2011)

visible, with Scer\GAL4^vg.PU

(Vallejo et al., 2011)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of mir-8^SC1 under the control of Scer\GAL4^Bx-MS1096 results in a small wing phenotype.

Expression of mir-8^SC1 under the control of Scer\GAL4^vg.PU results in a nicked wing phenotype.

Scer\GAL4[h.PU]Expression of mir-8^SC1 under the control of Scer\GAL4^h.PU results in denticle belt defects in the embryo.

Expression of mir-8^SC1 under the control of Scer\GAL4^ey.PH results in eyes with a very reduced ventral region and a profoundly distorted dorsal/ventral boundary. Apoptosis is seen in the ventral region of the eye disc during the first larval instar stage and in the organiser region of the eye disc at the late third larval instar stage in these animals.

(Vallejo et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ey.PH, mir-8^SC1 has visible phenotype, enhanceable by Ser[+]/Ser^RX106

(Vallejo et al., 2011)

Suppressed by

Statement

Reference

Scer\GAL4^ey.PH, mir-8^SC1 has visible phenotype, suppressible by Ser^UAS.cSa/Scer\GAL4^ey.PH

(Vallejo et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^ey.PH, mir-8^SC1 has visible phenotype, non-suppressible by Gug^UAS.cCa, Scer\GAL4^ey.PH

(Vallejo et al., 2011)

Suppressor of

Statement

Reference

mir-8^SC1, Scer\GAL4^ey.PH is a suppressor of neoplasia phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH, lola^GS88A8, psq^GS88A8

(Vallejo et al., 2011)

Other

Statement

Reference

Delta^UAS.cDa, Scer\GAL4^ey.PH, lola^GS88A8, mir-8^SC1, psq^GS88A8 has lethal | adult stage phenotype

(Vallejo et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ey.PH, mir-8^SC1 has eye phenotype, enhanceable by Ser[+]/Ser^RX106

(Vallejo et al., 2011)

Suppressed by

Statement

Reference

Scer\GAL4^ey.PH, mir-8^SC1 has eye ventral compartment phenotype, suppressible by Ser^UAS.cSa/Scer\GAL4^ey.PH

(Vallejo et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^ey.PH, mir-8^SC1 has eye ventral compartment phenotype, non-suppressible by Gug^UAS.cCa, Scer\GAL4^ey.PH

(Vallejo et al., 2011)

Suppressor of

Statement

Reference

mir-8^SC1, Scer\GAL4^ey.PH is a suppressor of eye phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH, lola^GS88A8, psq^GS88A8

(Vallejo et al., 2011)

mir-8^SC1, Scer\GAL4^ey.PH is a suppressor of eye-antennal disc phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH, lola^GS88A8, psq^GS88A8

(Vallejo et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Flies expressing Dl^Scer\UAS.cDa under the control of Scer\GAL4^ey.PH and also carrying the P{GSV1}lola^GS88A8 insertion (which causes overexpression of psq and lola) show an eye tumour phenotype. In addition, distant metastasis in the thorax and abdomen is seen in 15% of cases. Co-expression of mir-8^SC1 significantly reduces the primary eye tumour size, completely inhibits the incidence of metastases and results in a high incidence of adult lethality.

Co-expression of Ser^Scer\UAS.cSa suppresses the loss of ventral eye tissue caused by expression of mir-8^SC1 under the control of Scer\GAL4^ey.PH.

Co-expression of Gug^Scer\UAS.cCa does not suppress the loss of ventral eye tissue caused by expression of mir-8^SC1 under the control of Scer\GAL4^ey.PH.

Ser^RX106/+ markedly enhances the loss of eye tissue caused by expression of mir-8^SC1 under the control of Scer\GAL4^ey.PH.

(Vallejo et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

mir-8^SC1

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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