FB2024_03 , released June 25, 2024
Allele: Dmel\SerUAS.cSa
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General Information
Symbol
Dmel\SerUAS.cSa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Speicher
FlyBase ID
FBal0034019
Feature type
allele
Associated gene
Dmel\Ser
Associated Insertion(s)
Carried in Construct
P{UAS-Ser.mg5603}
Also Known As
UAS-Ser, UAS-Serrate, UASSer
Key Links
Transgenic product class
wild_type
(Thomas et al., 1995)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Thomas et al., 1995)
Mutagen
in vitro construct
(de Celis et al., 1998, Go et al., 1998, Klein and Martinez Arias, 1998, Milan et al., 1998, Thomas et al., 1995, Speicher et al., 1994)
Progenitor genotype
Carried in construct
P{UAS-Ser.mg5603}
Cytology
Description

UAS regulatory sequences drive expression of a Ser minigene (mg5603).

(Thomas et al., 1995)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Ser
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      dorsal row of wing sensilla, with Scer\GAL4ptc-559.1

      (de Celis et al., 1996)

      embryonic/larval crystal cell | larval stage | heat sensitive, with Scer\GAL4hs.PB

      (Duvic et al., 2002)

      embryonic/larval lymph gland, with Scer\GAL4FRT.Act5C

      (Lebestky et al., 2003)

      embryonic/larval lymph gland | larval stage, with Scer\GAL4hs.PB

      (Duvic et al., 2002)

      eye, with Scer\GAL4ey.PH

      (Reynolds-Kenneally and Mlodzik, 2005)

      femur, with Scer\GAL4dpp.blk1

      (de Celis et al., 1998)

      femur, with Scer\GAL4klu-G410

      (Bishop et al., 1999)

      leg, with Scer\GAL4Act5C.PI

      (Rauskolb and Irvine, 1999)

      leg, with Scer\GAL4klu-G410

      (Bishop et al., 1999)

      leg joint, with Scer\GAL4Act5C.PI

      (Rauskolb and Irvine, 1999)

      macrochaeta & wing, with Scer\GAL4hry-540.3

      (Speicher et al., 1994)

      macrochaeta & wing, with Scer\GAL4ptc-559.1

      (Speicher et al., 1994)

      macrochaeta | ectopic, with Scer\GAL4dpp.blk1

      (Klein et al., 1998)

      procrystal cell | ectopic, with Scer\GAL4FRT.Act5C

      (Lebestky et al., 2003)

      scutum, with Scer\GAL4Bx-MS1096

      (Klein and Martinez Arias, 1998)

      scutum & macrochaeta, with Scer\GAL4Bx-MS1096

      (Klein and Martinez Arias, 1998)

      tarsal segment 4, with Scer\GAL4klu-G410

      (Bishop et al., 1999)

      tarsal segment, with Scer\GAL4dpp.blk1

      (de Celis et al., 1998)

      tibia, with Scer\GAL4dpp.blk1

      (de Celis et al., 1998)

      tibia, with Scer\GAL4klu-G410

      (Bishop et al., 1999)

      ventral row of wing sensilla, with Scer\GAL4ptc-559.1

      (de Celis et al., 1996)

      wing, with Scer\GAL4ap-md544

      (Milan et al., 1998)

      wing, with Scer\GAL4Bx-MS1096

      (Klein et al., 1997)

      wing, with Scer\GAL4dpp.blk1

      (Klein and Martinez Arias, 1998)

      wing, with Scer\GAL4hry-540.3

      (Speicher et al., 1994)

      wing, with Scer\GAL4ptc-559.1

      (Lawrence et al., 2000, Klein and Martinez Arias, 1998, de Celis et al., 1996, Couso et al., 1995, Speicher et al., 1994)

      wing, with Scer\GAL4sd-SG29.1

      (Klein et al., 1997)

      wing, with Scer\GAL4vg.int2.1

      (Sun and Artavanis-Tsakonas, 1997)

      wing & macrochaeta, with Scer\GAL4dpp.blk1

      (Klein et al., 1998)

      wing & macrochaeta | dorsal, with Scer\GAL4Bx-MS1096

      (Klein et al., 1997)

      wing | ectopic, with Scer\GAL4ptc-559.1

      (Majumdar et al., 1997)

      wing | somatic clone, with Scer\GAL4Ubx.PdC

      (Baonza and Garcia-Bellido, 2000)

      wing | ventral, with Scer\GAL4ptc-559.1

      (Panin et al., 1997)

      wing | ventral, with Scer\GAL4Ubx.PdC

      (de Celis and Bray, 1997)

      wing disc, with Scer\GAL4dpp.blk1

      (Go et al., 1998)

      wing disc, with Scer\GAL4ptc-559.1

      (Go et al., 1998, Jonsson and Knust, 1996)

      wing disc | somatic clone, with Scer\GAL4Act5C.PI

      (Rauskolb et al., 1999)

      wing disc | ventral, with Scer\GAL4dpp.blk1

      (Lai et al., 2005)

      wing disc ventral compartment, with Scer\GAL4ptc-559.1

      (Majumdar et al., 1997)

      wing margin, with Scer\GAL4ptc-559.1

      (Panin et al., 1997)

      wing margin | ectopic, with Scer\GAL4ptc-559.1

      (Lawrence et al., 2000, Klein and Martinez Arias, 1998)

      wing margin bristle, with Scer\GAL4Bx-MS1096

      (Klein et al., 1997)

      wing sensillum | ectopic, with Scer\GAL4dpp.blk1

      (Klein and Martinez Arias, 1998)

      wing vein, with Scer\GAL4ap-md544

      (Milan et al., 1998)

      wing vein, with Scer\GAL4Bx-MS1096

      (Klein and Martinez Arias, 1998)

      wing vein, with Scer\GAL4Ubx.PdC

      (de Celis and Bray, 1997)

      wing vein | dorsal, with Scer\GAL4Bx-MS1096

      (Klein et al., 1997)

      wing vein | ectopic, with Scer\GAL4Ubx.PdC

      (de Celis and Bray, 1997)
      Detailed Description
      Statement
      Reference

      Expression of SerScer\UAS.cSa under the control of Scer\GAL4elav-C155 causes no obvious defects in the pattern of the major axon tracts in the embryo.

      (Thomas and van Meyel, 2007)

      Expression of SerScer\UAS.cSa, under the control of Scer\GAL4dpp.blk1, promotes ectopic wing margin and disc overgrowth in the ventral compartment.

      (Lai et al., 2005)

      Expression of SerScer\UAS.cSa, under the control of Scer\GAL4ey.PH, causes adult flies to have larger eyes than wild type.

      (Reynolds-Kenneally and Mlodzik, 2005)

      Expression of SerScer\UAS.cSa under the control of Scer\GAL4bi-omb-Gal4 does not affect eye development.

      (Singh et al., 2004)

      When SerScer\UAS.cSa clones are made in the lymph gland (driven by Scer\GAL4Scer\FRT.Act5C) a dramatic increase in crystal cell precursors is seen the second lobe (where they are rarely seen in wild-type).

      (Lebestky et al., 2003)

      Increased numbers of crystal cells are seen in heat shocked SerScer\UAS.cSa; Scer\GAL4hs.PB larvae at late third instar. These larvae also have increased numbers of prophenoloxidase expressing cells in the larval lymph glands, as do SerScer\UAS.cSa; Scer\GAL4[e33C] larvae.

      (Duvic et al., 2002)

      Expression of SerScer\UAS.cSa under the control of Scer\GAL4byn-Gal4 does not have an effect on the formation of the hindgut boundary cells.

      (Iwaki and Lengyel, 2002)

      Clones in the eye disc expressing SerScer\UAS.cSa under the control of Scer\GAL4Act5C.PI do not induce ectopic neural differentiation ahead of the morphogenetic furrow.

      (Baonza and Freeman, 2001)

      Somatic clones of SerScer\UAS.cSa expressing cells (driven by Scer\GAL4Ubx.PdC or Scer\GAL4Act5C.PI) in the wing pouch of the ventral surface (but not the dorsal surface) produce outgrowths in the adult wing.

      (Baonza and Garcia-Bellido, 2000)

      Expression of SerScer\UAS.cSa under the control of Scer\GAL4ptc-559.1 results in a small wing with an ectopic margin along the anterior/posterior boundary on the ventral surface. The ectopic margin shows a nick where it meets the endogenous margin.

      (Lawrence et al., 2000)

      Expression of SerScer\UAS.cSa under the control of Scer\GAL4klu-G410 results in pharate adults and escapers at 25oC. Ectopic development of joint-like cuticle is seen on the legs, characterised by loss of bristles, cuticle thickenings and inpocketings. Ectopic joints are seen in the tibia, femur and fourth tarsal segment.

      (Bishop et al., 1999)

      Formation of normal joints is inhibited in clones in the leg expressing SerScer\UAS.cSa under the control of Scer\GAL4Act5C.PI. The clones can also result in outgrowth of leg tissue. The outgrowth is composed of a mixture of wild-type cells and cells expressing SerScer\UAS.cSa under the control of Scer\GAL4Act5C.PI. Ectopic joints are also seen.

      (Rauskolb and Irvine, 1999)

      Flip-out somatic clones in the wing disc, in which there is clonal ectopic expression of SerScer\UAS.cSa, lead to variable results. Those clones that touch the dorsal-ventral boundary and inhibit endogenous N activity always disturb dorsal-ventral compartmentalization, whereas most that appear to leave endogenous activation intact do not disturb compartmentalization.

      (Rauskolb et al., 1999)

      The wing pouch is enlarged in larvae expressing SerScer\UAS.cSa under the control of either Scer\GAL4dpp.blk1 or Scer\GAL4ptc-559.1, and cells in the peripheral region of the wing pouch actively divide.

      (Go et al., 1998)

      Flies expressing SerScer\UAS.cSa under the control of Scer\GAL4dpp.blk1 have a very few ectopic bristles on the ventral side of the wing pouch next to the normal wing margin. Flies expressing SerScer\UAS.cSa under the control of Scer\GAL4ptc-559.1 have smaller wings than normal which have two ectopic wing margins. Flies expressing SerScer\UAS.cSa under the control of Scer\GAL4Bx-MS1096 show broadening of the wing veins and a moderate increase in the number of bristles on the notum.

      (Klein and Martinez Arias, 1998)

      Scer\GAL4dpp.blk1-mediated ectopic expression has minimal effects on the pattern of the wing. Sometimes a tuft of ectopic bristles can be observed at the intersection of the AP boundary with the wing margin.

      (Klein et al., 1998)

      Flies expressing SerScer\UAS.cSa under the control of Scer\GAL4ap-md544 have small wings with thickened veins.

      (Milan et al., 1998)

      Expression of SerScer\UAS.cSa under the control of Scer\GAL4dpp.blk1 results in a foreshortened tibia which forms abnormal joints with the femur and tarsi. Incomplete ectopic joints are occasionally seen in distal tarsal segments.

      (de Celis et al., 1998)

      Scer\GAL4sd-SG29.1-mediated expression curtails wing development and produces wings that lack most of the margin and adjacent tissue. Scer\GAL4Bx-MS1096-mediated expression produces wings smaller than wild type that lack most of the margin and adjacent tissue and display extra bristles at the margin and thickened veins on the dorsal side.

      (Klein et al., 1997)

      Scer\GAL4ptc-559.1-mediated expression causes an ectopic outgrowth of the wing pouch in the ventral compartment.

      (Majumdar et al., 1997)

      Ectopic wing margin is formed in ventral cells of the wing when SerScer\UAS.cSa is expressed using Scer\GAL4ptc-559.1.

      (Panin et al., 1997)

      Replacement of Dl zygotic expression using Scer\GAL4arm.T:Hsim\VP16-mediated expression in DlRevF10 embryos allows some rescue of the CNS but it is reduced at later stages.

      (Seugnet et al., 1997)

      When expressed via Scer\GAL4vg.int2.1 the wing blades are expanded, particularly at the posterior margin. Can suppress the missing wing blade phenotype caused by SerS.Scer\UAS.T:Hsap\MYC.

      (Sun and Artavanis-Tsakonas, 1997)

      When expression is driven by Scer\GAL4Ubx.PdC outgrowths of the wing occur only when the clone is induced in the ventral surface of the wing. Ventral wing clones induce ectopic wing veins at their boundaries, which mainly develop from wild type cells. Thickening of wing veins and notches in the wing margin also occur. Clones on the dorsal surface cause the differentiation of thicker veins.

      (de Celis and Bray, 1997)

      Scer\GAL4ptc-559.1-mediated expression in third instar wild type wing discs causes outgrowth on the ventral side and induction of a new margin, or a nick of the wing margin.

      (Jonsson and Knust, 1996)

      When expression is governed by Scer\GAL4ptc-559.1 in the wing disc, wing shows overgrowth and ectopic margin structures on the ventral surface.

      (de Celis et al., 1996)

      Scer\GAL4ptc-559.1 induced expression causes overgrowth of the ventral wing and formation of ectopic wing margin.

      (Couso et al., 1995)

      When expressed under the influence of Scer\GAL4h-540.3 or Scer\GAL4ptc-559.1, temperature-dependent overgrowth of the wing results. For Scer\GAL4h-540.3, the bulk of the extra tissue projects onto the ventral surface close to the anterior hinge region. For Scer\GAL4ptc-559.1 the additional wing tissue is found between the third and fourth wing vein, usually forming a large blister on the ventral wing blade. The additional tissue is accompanied by rows or patches of bristles. Extra tissue shows no sign of neoplasic overgrowth, and no difference in cell size from wild type. The phenocritical period for the overgrowth phenotype is between the mid-second and mid-third larval instar.

      (Speicher et al., 1994)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Enhancer of
      Statement
      Reference

      Scer\GAL4Bx-hdpR590/SerUAS.cSa is an enhancer of visible | recessive phenotype of BxhdpR590

      (Milan and Cohen, 2000)
      Suppressor of
      Other
      Phenotype Manifest In
      NOT Enhanced by
      Suppressed by
      Statement
      Reference

      Scer\GAL4dpp.blk1, SerUAS.cSa has wing disc | ventral phenotype, suppressible by mib1ΔRF.UAS.Tag:polyHis,Tag:MYC/Scer\GAL4dpp.blk1

      (Lai et al., 2005)

      Scer\GAL4ey.PH, SerUAS.cSa has eye phenotype, suppressible by eyg685/eyg[+]

      (Reynolds-Kenneally and Mlodzik, 2005)

      Scer\GAL4ey.PH, SerUAS.cSa has eye phenotype, suppressible by Df(1)N-54l9/+

      (Reynolds-Kenneally and Mlodzik, 2005)

      Scer\GAL4ey.PH, SerUAS.cSa has eye phenotype, suppressible by hop[+]/hop25

      (Reynolds-Kenneally and Mlodzik, 2005)

      Scer\GAL4ey.PH, SerUAS.cSa has eye phenotype, suppressible by upd154/os[+]

      (Reynolds-Kenneally and Mlodzik, 2005)

      Scer\GAL4ptc-559.1, SerUAS.cSa has wing margin | ectopic phenotype, suppressible by NECNΔ10-12.UAS, Scer\GAL4ptc-559.1

      (Lawrence et al., 2000)

      Scer\GAL4ptc-559.1, SerUAS.cSa has wing margin | ectopic phenotype, suppressible by NECN.UAS, Scer\GAL4ptc-559.1

      (Lawrence et al., 2000)

      Scer\GAL4ptc-559.1, SerUAS.cSa has wing margin | ectopic phenotype, suppressible | partially by NECNΔ17-19.24-26.UAS, Scer\GAL4ptc-559.1

      (Lawrence et al., 2000)

      Scer\GAL4ptc-559.1, SerUAS.cSa has wing phenotype, suppressible by NECNΔ10-12.UAS, Scer\GAL4ptc-559.1

      (Lawrence et al., 2000)

      Scer\GAL4ptc-559.1, SerUAS.cSa has wing phenotype, suppressible by NECN.UAS, Scer\GAL4ptc-559.1

      (Lawrence et al., 2000)

      Scer\GAL4ptc-559.1, SerUAS.cSa has wing phenotype, suppressible | partially by NECNΔ17-19.24-26.UAS, Scer\GAL4ptc-559.1

      (Lawrence et al., 2000)

      Scer\GAL4ptc-559.1, SerUAS.cSa has wing margin | ectopic phenotype, suppressible by Scer\GAL4ptc-559.1/fngUAS.cKa

      (Klein and Martinez Arias, 1998)

      Scer\GAL4Bx-MS1096, SerUAS.cSa has wing vein phenotype, suppressible by Scer\GAL4Bx-MS1096/fngUAS.cKa

      (Klein and Martinez Arias, 1998)

      Scer\GAL4dpp.blk1, SerUAS.cSa has wing phenotype, suppressible | partially by apUGO35/apUGO35

      (Klein and Martinez Arias, 1998)

      Scer\GAL4ptc-559.1, SerUAS.cSa has wing disc phenotype, suppressible by sno71e1

      (Majumdar et al., 1997)
      NOT suppressed by
      Enhancer of
      NOT Enhancer of
      Statement
      Reference

      Scer\GAL4dpp.blk1/SerUAS.cSa is a non-enhancer of wing phenotype of vg83b27-R

      (Klein and Martinez Arias, 1998)
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4Ser.PGF/SerUAS.cSa is a non-suppressor of wing disc phenotype of mib13/mib1EY09780

      (Le Borgne et al., 2005)

      Scer\GAL4dpp.blk1, SerUAS.cSa, vgUAS.cKa is a non-suppressor of wing disc phenotype of Su(H)8/Su(H)2

      (Klein et al., 2000)
      Other
      Statement
      Reference

      Scer\GAL4bi-omb-Gal4, SerUAS.cSa, tshUAS.cGa has eye | dorsal phenotype

      (Singh et al., 2004)

      Scer\GAL4bi-omb-Gal4, SerUAS.cSa, tshUAS.cGa has eye phenotype

      (Singh et al., 2004)

      Scer\GAL4bi-omb-Gal4, SerUAS.cSa, tshUAS.cGa has eye disc phenotype

      (Singh et al., 2004)

      Scer\GAL4bi-omb-Gal4, SerUAS.cSa, tshUAS.cGa has eye | ventral phenotype

      (Singh et al., 2004)

      Scer\GAL4bi-omb-Gal4, SerUAS.cSa, tshUAS.cGa has eye disc | dorsal phenotype

      (Singh et al., 2004)

      Scer\GAL4bi-omb-Gal4, SerUAS.cSa, tshUAS.cGa has eye disc | ventral phenotype

      (Singh et al., 2004)

      Chie5.5, Scer\GAL4Bx-MS1096, SerUAS.cSa has wing disc phenotype

      (Milan and Cohen, 2000)

      Scer\GAL4ap-md544, SerUAS.cSa, apmd544/apUGO35, fngEP3082 has wing phenotype

      (Milan and Cohen, 1999)

      Scer\GAL4ap-md544, SerUAS.cSa, apmd544/apUGO35 has wing disc phenotype

      (Milan and Cohen, 1999)

      Scer\GAL4ap-md544, SerUAS.cSa, apmd544/apUGO35 has wing margin bristle | ectopic phenotype

      (Milan and Cohen, 1999)

      Scer\GAL4dpp.blk1, SerUAS.cSa, vg83b27-R, vgUAS.cKa has wing phenotype

      (Klein and Martinez Arias, 1998)

      Scer\GAL4dpp.blk1, SerUAS.cSa, apUGO35 has wing | ectopic phenotype

      (Klein et al., 1998)

      Scer\GAL4ptc-559.1, SerUAS.cSa, fngUAS.cKa has wing phenotype

      (Panin et al., 1997)

      Scer\GAL4ptc-559.1, SerUAS.cSa, fngD4 has wing | ventral phenotype

      (Panin et al., 1997)

      Scer\GAL4ptc-559.1, SerUAS.cSa, wgl-12.UAS has eye-antennal disc phenotype

      (Jonsson and Knust, 1996)

      Scer\GAL4ptc-559.1, SerUAS.cSa, wgl-12.UAS has wing disc phenotype

      (Jonsson and Knust, 1996)

      Scer\GAL4ptc-559.1, SerUAS.cSa, wgl-12.UAS has leg disc phenotype

      (Jonsson and Knust, 1996)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of SerScer\UAS.cSa suppresses the loss of ventral eye tissue caused by expression of mir-8SC1 under the control of Scer\GAL4ey.PH.

      (Vallejo et al., 2011)

      The additional expression of mib1Scer\UAS.T:Zzzz\His6,T:Hsap\MYC in wing discs expressing SerScer\UAS.cSa under the control of Scer\GAL4dpp.blk1 does not change the wing disc phenotype. In contrast, the wing phenotype caused by SerScer\UAS.cSa under the control of Scer\GAL4dpp.blk1 is completely suppressed by mib1ΔRF.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC coexpression.

      (Lai et al., 2005)

      The following heterozygous mutations suppress the large eye phenotype of Scer\GAL4ey.PH > SerScer\UAS.cSa flies: eyg685, Df(1)N-54l9, upd154 and hop25. Heterozygosity for either domeG0218, domeG0282 or Stat92Ej6C8 fails to suppress this phenotype.

      (Reynolds-Kenneally and Mlodzik, 2005)

      The Bx1 scalloped wing phenotype is suppressed by expression of SerScer\UAS.cSa under the control of Scer\GAL4ap-md544.

      (Milan et al., 2004)

      Co-expression of both tshScer\UAS.cGa and SerScer\UAS.cSa under the control of Scer\GAL4bi-omb-Gal4 suppresses eye development in both the dorsal and ventral margins of the eye disc and adult eye. However, despite this suppression of photoreceptor differentiation, the eye disc shows overall enlargement and the adult eye is also enlarged and folded.

      (Singh et al., 2004)

      The wing phenotype caused by expression of SerScer\UAS.cSa under the control of Scer\GAL4ptc-559.1 is suppressed by coexpression of NECN.Scer\UAS, NECNΔ10-12.Scer\UAS or NECNΔ17-19;24-26.Scer\UAS (although suppression is not always complete in the case of NECNΔ17-19;24-26.Scer\UAS).

      (Lawrence et al., 2000)

      Chie5.5 clones in the wing disc of animals expressing SerScer\UAS.cSa under the control of Scer\GAL4Bx-MS1096 cause axis bifurcation. The severity of the BxhdpR590/Y wing phenotype is enhanced by expression of SerScer\UAS.cSa under the control of Scer\GAL4Bx-hdpR590.

      (Milan and Cohen, 2000)

      apmd544/apUGO35 flies show reduction of the wing and complete loss of wing margin. Expression of SerScer\UAS.cSa under the control of Scer\GAL4ap-md544 in these flies restores the wing margin and supports growth of the wing. Patches of ectopic wing margin are found in both surfaces of the rescued wings and overgrowth of the wing can be seen. Groups of cells appear to violate the dorsoventral boundary in apmd544/apUGO35 wing discs which are also expressing SerScer\UAS.cSa under the control of Scer\GAL4ap-md544. The mispositioned patches of dorsal or ventral cells form relatively smooth interfaces with surrounding cells. Expression of both SerScer\UAS.cSa and fngEP3082 under the control of Scer\GAL4ap-md544 in apmd544/apUGO35 flies results in well-developed wings which differentiate ventral features on both surfaces; the wing margin, veins and sense organs on the topologically dorsal surface have exclusively ventral identity.

      (Milan and Cohen, 1999)

      The ectopic wing margin structures seen in flies expressing SerScer\UAS.cSa under the control of Scer\GAL4ptc-559.1 are reduced to a posterior stripe with characteristics of the posterior compartment if the flies are also co-expressing fngScer\UAS.cKa. The loss of wing seen in apUGO35 homozygotes is partially rescued by SerScer\UAS.cSa expressed under the control of Scer\GAL4dpp.blk1. The extra vein phenotype seen in flies expressing SerScer\UAS.cSa under the control of Scer\GAL4Bx-MS1096 is suppressed if the flies are also expressing fngScer\UAS.cKa.

      (Klein and Martinez Arias, 1998)

      Mediated expression in apUGO35 mutants elicits an ectopic wing. Wing consists of dorsal and ventral cells and the wing margin cells are on the dorsal side.

      (Klein et al., 1998)

      Coexpression of NECN.Scer\UAS and SerScer\UAS.cSa under the control of Scer\GAL4sd-SG29.1 demonstrates Ser can partially suppress the effect of N : wings are smaller than wild type, the dorsal surface is smaller than the ventral surface. Coexpression of wgl-12.Scer\UAS and SerScer\UAS.cSa with Scer\GAL4sd-SG29.1 the resulting flies bear large wings that are covered in bristles. The wings lack a defined margin and are more round that elongated along the PD axis. Coexpression with Scer\GAL4ptc-559.1 leads to the production of extra wings off the proximal dorsal side of the wing. Coexpression of SerScer\UAS.cSa and NScer\UAS.cBb under the control of Scer\GAL4sd-SG29.1 generates very large wings with a clearly defined margin with an antineurogenic phenotype. Coexpression under the control of Scer\GAL4Bx-MS1096 results in normally shaped wings in which the dorsal surface has overgrown the ventral one. Coexpression under the control of Scer\GAL4ptc-559.1 causes the ectopic ventral double margin characteristic of SerScer\UAS.cSa alone to be transformed into a single thick one, and the ventral margin and associated overgrowth are extended into the dorsal surface of the wing.

      (Klein et al., 1997)

      Introduction of sno71e1 entirely suppresses the outgrowth seen in flies expressing SerScer\UAS.cSa under the control of Scer\GAL4ptc-559.1.

      (Majumdar et al., 1997)

      The ectopic wing margin phenotype caused by expression of SerScer\UAS.cSa under the control of Scer\GAL4ptc-559.1 is partly suppressed in flies also carrying fngD4. Co-expression of fngScer\UAS.cKa with SerScer\UAS.cSa in the wing (using Scer\GAL4ptc-559.1) stimulates ectopic wing margin formation.

      (Panin et al., 1997)

      Scer\GAL4ptc-559.1-mediated coexpression with NECN.Scer\UAS allows most wild-type flies to hatch at 25oC, both phenotypes provoked by SerScer\UAS.cSa alone are strongly suppressed. Scer\GAL4ptc-559.1-mediated coexpression with wgl-12.Scer\UAS causes outgrowth on the ventral side of the wing discs and a small amount on the dorsal side but the nick in the wing margin is repressed. Ectopic wing tissue is also found in the compound eye and legs. Scer\GAL4h-540.3-mediated expression of SerScer\UAS.cSa in apunspecified second/early third larval instar partially restores wing formation (about half normal length but lack any marginal differentiation).

      (Jonsson and Knust, 1996)

      Scer\GAL4ptc-559.1 induced co-expression of wgl-12.Scer\UAS and SerScer\UAS.cSa causes a duplicated pair of extra wings that arise from the proximal dorsal side of the endogenous wings. The endogenous wings may exhibit extra margin material. Wing tissues appear, with characteristic marginal bristles, from the proximal dorsal side in all legs. Scer\GAL4h-1J3 induced coexpression of wgl-12.Scer\UAS and SerScer\UAS.cSa fails to cause ectopic wings, expression of NScer\UAS.cBb with both wgl-12.Scer\UAS and SerScer\UAS.cSa causes ectopic wing development.

      (Couso et al., 1995)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      SerUAS.cSa/Scer\GAL4tin.CΔ4 rescues Df(2L)Exel7007

      (Kim et al., 2010)

      SerUAS.cSa/Scer\GAL4tin.CΔ4 rescues SerVDRC.cUa

      (Kim et al., 2010)

      SerUAS.cSa/Scer\GAL4vg.MQ.Exel rescues SerVDRC.cUa

      (Kim et al., 2010)
      Partially rescues

      Scer\GAL4klu-G410/SerUAS.cSa partially rescues Serunspecified

      (Klein and Martinez Arias, 1998)

      Scer\GAL4hry-540.3/SerUAS.cSa partially rescues SerRX82/Df(3R)D605

      (Jonsson and Knust, 1996)
      Comments

      Expression of SerScer\UAS.cSa under the control of Scer\GAL4tin.CΔ4 rescues the abnormal cardiac function of Df(2L)Exel7007 flies.

      Co-expression of SerScer\UAS.cSa in flies expressing SerVDRC.cUa under the control of Scer\GAL4tin.CΔ4 rescues the abnormal cardiac function phenotype.

      Co-expression of SerScer\UAS.cSa in flies expressing SerVDRC.cUa under the control of Scer\GAL4vg.MQ.Exel rescues the notched-wing phenotype.

      (Kim et al., 2010)

      Scer\GAL4dpp.blk1-mediated coexpression with vgScer\UAS.cKa in vg83b27-R mutants leads to significant induction of an ectopic wing margin.

      (Klein and Martinez Arias, 1998)

      Scer\GAL4h-540.3-mediated expression of SerScer\UAS.cSa in SerRX82/Df(3R)D605 or second/early third larval instar partially restores wing formation.

      (Jonsson and Knust, 1996)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Ectopic activation of Ser by Scer\GAL4ptc-559.1 or Scer\GAL4salm-459.2 results in ectopic activation of N along the boundary of Ser expression in the ventral part of the wing disc.

      (de Celis et al., 1996)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      SerScer\UAS.cSa
      SerUAS.cSa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Speicher
      Secondary FlyBase IDs
        References (69)