FlyBase Allele Report: Dmel\N[ECNΔ17-19.24-26.UAS]

General Information

Symbol

Dmel\N^{ECNΔ17-19.24-26.UAS}

Species

D. melanogaster

Name

FlyBase ID

FBal0104078

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

P{UAS-N.ECNΔ17-19.24-26}

Key Links

Transgenic product class

inframe_deletion

(Brennan et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Brennan et al., 1999)

Mutagen

in vitro construct

(Brennan et al., 1999)

Progenitor genotype

N^ECN.UAS

(Brennan et al., 1999)

Carried in construct

P{UAS-N.ECNΔ17-19.24-26}

Cytology

Description

A deletion of amino acids 652-803 inclusive removes EGF-like repeats 17 to 19. A second deletion of amino acids 921-1072 inclusive removes EGF-like repeats 24 to 26.

(Brennan et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^ptc-559.1

(Lawrence et al., 2000)

visible, with Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

visible | neurogenic phenotype, with Scer\GAL4^ptc-559.1

(Brennan et al., 1999)

Phenotype Manifest In

macrochaeta | ectopic, with Scer\GAL4^ptc-559.1

(Brennan et al., 1999)

wing, with Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

wing disc, with Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

wing margin bristle, with Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

wing vein L3, with Scer\GAL4^ptc-559.1

(Lawrence et al., 2000)

wing vein L4, with Scer\GAL4^ptc-559.1

(Lawrence et al., 2000)

Detailed Description

Statement

Reference

Expression of N^{ECNΔ17-19;24-26.Scer\UAS} under the control of Scer\GAL4^ptc-559.1 results in a fusion between wing veins 3 and 4.

(Lawrence et al., 2000)

When expression of N^{ECNΔ17-19;24-26.Scer\UAS} is driven by Scer\GAL4^sd-SG29.1 the wing disc has a small wing pouch. Adult wings lack the majority of both the anterior and posterior wing margin.

(Brennan et al., 1999)

Expression of N^{ECNΔ17-19;24-26.Scer\UAS} driven by Scer\GAL4^ptc-559.1 at 25^oC results in a neurogenic phenotype with many more scutellar bristles differentiating than wild-type.

(Brennan et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

N^{ECNΔ17-19.24-26.UAS}, Scer\GAL4^ptc-559.1 is a suppressor | partially of visible phenotype of Scer\GAL4^ptc-559.1, Ser^UAS.cSa

(Lawrence et al., 2000)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ptc-559.1/N^{ECNΔ17-19.24-26.UAS} is a non-suppressor of visible phenotype of Delta^UAS.cHa

(Lawrence et al., 2000)

Phenotype Manifest In

Suppressed by

Statement

Reference

N^{ECNΔ17-19.24-26.UAS}, Scer\GAL4^sd-SG29.1 has wing disc phenotype, suppressible | partially by wg^l-12.UAS, Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

N^{ECNΔ17-19.24-26.UAS}, Scer\GAL4^sd-SG29.1 has wing phenotype, suppressible | partially by wg^l-12.UAS, Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

Suppressor of

Statement

Reference

N^{ECNΔ17-19.24-26.UAS}, Scer\GAL4^ptc-559.1 is a suppressor | partially of wing margin | ectopic phenotype of Scer\GAL4^ptc-559.1, Ser^UAS.cSa

(Lawrence et al., 2000)

N^{ECNΔ17-19.24-26.UAS}, Scer\GAL4^ptc-559.1 is a suppressor | partially of wing phenotype of Scer\GAL4^ptc-559.1, Ser^UAS.cSa

(Lawrence et al., 2000)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ptc-559.1/N^{ECNΔ17-19.24-26.UAS} is a non-suppressor of wing margin | ectopic phenotype of Delta^UAS.cHa

(Lawrence et al., 2000)

Scer\GAL4^ptc-559.1/N^{ECNΔ17-19.24-26.UAS} is a non-suppressor of wing phenotype of Delta^UAS.cHa

(Lawrence et al., 2000)

Other

Statement

Reference

N^{ECNΔ17-19.24-26.UAS}, Scer\GAL4^sd-SG29.1, wg^UAS.cGa has sensory mother cell & dorsal mesothoracic disc | ectopic phenotype

(Brennan et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

The wing phenotype caused by expression of Ser^Scer\UAS.cSa under the control of Scer\GAL4^ptc-559.1 is suppressed by coexpression of N^{ECNΔ17-19;24-26.Scer\UAS}, although suppression is not always complete. The wing phenotype caused by expression of Dl^Scer\UAS.cHa under the control of Scer\GAL4^ptc-559.1 is not suppressed by coexpression of N^{ECNΔ17-19;24-26.Scer\UAS}.

(Lawrence et al., 2000)

The addition of wg^{l-12.Scer\UAS} in animals expressing N^{ECNΔ17-19;24-26.Scer\UAS} driven by Scer\GAL4^sd-SG29.1 restores the wing pouch almost to wild-type, and partially suppresses the adult wing phenotype, though the wing margin remains severely disrupted. When expression of N^{ECNΔ17-19;24-26.Scer\UAS} and wg^Scer\UAS.cGa are driven by Scer\GAL4^sd-SG29.1, the whole of the anterior compartment of the wing disc is covered in SOPs (sensory organ precursors). However the spacing normally seen between SOPs is abolished.

(Brennan et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

N^{ECNΔ17-19.24-26.Scer\UAS}

N^{ECNΔ17-19.24-26.UAS}

N^{ECNΔ17-19;24-26.Scer\UAS}

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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