FlyBase Allele Report: Dmel\Delta[UAS.cHa]

General Information

Symbol

Dmel\Delta^UAS.cHa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Haenlin

FlyBase ID

FBal0059140

Feature type

allele

Associated gene

Dmel\Delta

Associated Insertion(s)

Carried in Construct

P{UAS-Delta.H}

Also Known As

UAS-Dl, UAS-Dl.H, UASDl, UAS-Delta

Key Links

Transgenic product class

wild_type

(Seugnet et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Seugnet et al., 1997)

Mutagen

in vitro construct

(Jacobsen et al., 1998, Klein and Martinez Arias, 1998, Seugnet et al., 1997, Jonsson and Knust, 1996)

Progenitor genotype

Carried in construct

P{UAS-Delta.H}

Cytology

Description

UASt regulatory sequences drive expression of Dl.

(Seugnet et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Delta

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity, with Scer\GAL4^hs.2sev

(Fanto and Mlodzik, 1999)

lethal - all die before end of P-stage, with Scer\GAL4^ptc-559.1

(Jonsson and Knust, 1996)

some die during pharate adult stage, with Scer\GAL4^ptc-559.1

(Jonsson and Knust, 1996)

visible, with Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

visible, with Scer\GAL4^ptc-559.1

(Lawrence et al., 2000)

Phenotype Manifest In

cone cell | increased number

(Nagaraj and Banerjee, 2009)

egg chamber, with Scer\GAL4^upd1-E132

(Assa-Kunik et al., 2007)

eye, with Scer\GAL4^ey.PH

(Reynolds-Kenneally and Mlodzik, 2005)

leg disc, with Scer\GAL4^ptc-559.1

(Jonsson and Knust, 1996)

ommatidium, with Scer\GAL4^hs.2sev

(Fanto and Mlodzik, 1999)

photoreceptor cell R1

(Nagaraj and Banerjee, 2009)

photoreceptor cell R1, with Scer\GAL4^hs.2sev

(Fanto and Mlodzik, 1999)

photoreceptor cell R2, with Scer\GAL4^hs.2sev

(Fanto and Mlodzik, 1999)

photoreceptor cell R3, with Scer\GAL4^hs.2sev

(Fanto and Mlodzik, 1999)

photoreceptor cell R4, with Scer\GAL4^hs.2sev

(Fanto and Mlodzik, 1999)

photoreceptor cell R5, with Scer\GAL4^hs.2sev

(Fanto and Mlodzik, 1999)

photoreceptor cell R6

(Nagaraj and Banerjee, 2009)

photoreceptor cell R6, with Scer\GAL4^hs.2sev

(Fanto and Mlodzik, 1999)

photoreceptor cell R7

(Nagaraj and Banerjee, 2009)

polar follicle cell | increased number, with Scer\GAL4^neur-P72

(Assa-Kunik et al., 2007)

posterior supraalar bristle & trichogen cell, with Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

primary pigment cell | increased number

(Nagaraj and Banerjee, 2009)

sensory mother cell & filopodium, with Scer\GAL4^neur-P72

(De Joussineau et al., 2003)

stalk follicle cell, with Scer\GAL4^upd1-E132

(Assa-Kunik et al., 2007)

wing, with Scer\GAL4^ptc-559.1

(Lawrence et al., 2000)

wing disc, with Scer\GAL4^ptc-559.1

(Jonsson and Knust, 1996)

wing margin | ectopic, with Scer\GAL4^ptc-559.1

(Lawrence et al., 2000)

Detailed Description

Statement

Reference

Expression of Dl^Scer\UAS.cHa in the third instar eye discs (under the control of Scer\GAL4^elav-C155) results in a loss in the specification of R1, R6 and R7 and an over-specification of non-neuronal cone and pigment cell types.

(Nagaraj and Banerjee, 2009)

Expression of Dl^Scer\UAS.cHa under the control of Scer\GAL4^neur-P72 results in extra polar follicle cells.

Expression of Dl^Scer\UAS.cHa under the control of Scer\GAL4^upd-E132 results in loss of the stalk between egg chambers and fusion of neighbouring chambers.

(Assa-Kunik et al., 2007)

Expression of Dl^Scer\UAS.cHa in a Ubx^lac1-GAL4 background (the transgene expression is driven by the P{GawB} in the insertion that generates the Ubx^lac1-GAL4 allele) enhances the haltere phenotype of Ubx^lac1-GAL4 flies, leading to a large number of wing-type bristle and trichomes on the haltere.

(Mohit et al., 2006)

Expression of Dl^Scer\UAS.cHa, under the control of Scer\GAL4^ey.PH, causes adult flies to have larger eyes than wild type.

(Reynolds-Kenneally and Mlodzik, 2005)

Expression of Dl^Scer\UAS.cHa under the control of Scer\GAL4^bi-omb-Gal4 does not affect eye size.

(Singh et al., 2004)

The number and length of filopodia formed by sensory organ precursors in wing disc cells at the time puparium formation is dramatically increased in Dl^Scer\UAS.cHa; Scer\GAL4^neur-P72 flies compared to wild-type.

(De Joussineau et al., 2003)

Expression of Dl^Scer\UAS.cHa under the control of Scer\GAL4^ptc-559.1 results in a small wing with an ectopic margin along the anterior/posterior boundary on both surfaces of the wing.

(Lawrence et al., 2000)

Flies expressing Dl^Scer\UAS.cHa under the control of Scer\GAL4^hs.2sev develop many symmetrical ommatidial clusters showing the R4/R4 phenotype. The development of outer photoreceptors R1 to R6 is also affected.

(Fanto and Mlodzik, 1999)

Transformation of the trichogen to a tormogen fate is seen in notal macrochaetae of flies expressing Dl^Scer\UAS.cHa under the control of Scer\GAL4^{l(3)31-1-31-1}, including the anterior dorsocentral and posterior supraalar bristles.

(Jacobsen et al., 1998)

Scer\GAL4^ptc-559.1-mediated expression in wild type at 18^oC causes pharate adult lethality, mutants exhibit severe defects in the wings and legs which often do not evert and are occasionally duplicated. In less severe cases wings have extra tissue on the ventral and dorsal sides, with a new margin on both sides which runs perpendicular to and crosses the endogenous margin. Wings do not exhibit margin nicks. Ectopic wings develop in the head.

(Jonsson and Knust, 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Delta^UAS.cHa has visible phenotype, suppressible by Scer\GAL4^ptc-559.1/N^ECN.UAS

(Lawrence et al., 2000)

NOT suppressed by

Statement

Reference

Delta^UAS.cHa has visible phenotype, non-suppressible by Scer\GAL4^ptc-559.1/N^{ECNΔ10-12.UAS}

(Lawrence et al., 2000)

Delta^UAS.cHa has visible phenotype, non-suppressible by Scer\GAL4^ptc-559.1/N^{ECNΔ17-19.24-26.UAS}

(Lawrence et al., 2000)

Enhancer of

Statement

Reference

Delta^UAS.cHa/Scer\GAL4^{Ubx-lac1-Gal4} is an enhancer of visible | homeotic phenotype of Ubx^lac1-GAL4

(Mohit et al., 2006)

Suppressor of

Statement

Reference

Delta^UAS.cHa/Scer\GAL4^dpp.blk1 is a suppressor of visible | recessive phenotype of ap^UGO35

(Klein and Martinez Arias, 1998)

Phenotype Manifest In

Suppressed by

Statement

Reference

Delta^UAS.cHa, Scer\GAL4^ey.PH has eye phenotype, suppressible by Df(1)N-54l9/+

(Reynolds-Kenneally and Mlodzik, 2005)

Delta^UAS.cHa, Scer\GAL4^ey.PH has eye phenotype, suppressible by hop[+]/hop²⁵

(Reynolds-Kenneally and Mlodzik, 2005)

Delta^UAS.cHa, Scer\GAL4^ey.PH has eye phenotype, suppressible by upd1⁵⁴/os[+]

(Reynolds-Kenneally and Mlodzik, 2005)

Delta^UAS.cHa, Scer\GAL4^ey.PH has eye phenotype, suppressible by eyg⁶⁸⁵/eyg[+]

(Reynolds-Kenneally and Mlodzik, 2005)

Delta^UAS.cHa has wing margin | ectopic phenotype, suppressible by Scer\GAL4^ptc-559.1/N^ECN.UAS

(Lawrence et al., 2000)

Delta^UAS.cHa has wing phenotype, suppressible by Scer\GAL4^ptc-559.1/N^ECN.UAS

(Lawrence et al., 2000)

NOT suppressed by

Statement

Reference

Delta^UAS.cHa, Scer\GAL4^ey.PH has eye phenotype, non-suppressible by dome[+]/dome^G0218

(Reynolds-Kenneally and Mlodzik, 2005)

Delta^UAS.cHa, Scer\GAL4^ey.PH has eye phenotype, non-suppressible by dome[+]/dome^G0282

(Reynolds-Kenneally and Mlodzik, 2005)

Delta^UAS.cHa has wing phenotype, non-suppressible by Scer\GAL4^ptc-559.1/N^{ECNΔ17-19.24-26.UAS}

(Lawrence et al., 2000)

Delta^UAS.cHa has wing margin | ectopic phenotype, non-suppressible by Scer\GAL4^ptc-559.1/N^{ECNΔ10-12.UAS}

(Lawrence et al., 2000)

Delta^UAS.cHa has wing margin | ectopic phenotype, non-suppressible by Scer\GAL4^ptc-559.1/N^{ECNΔ17-19.24-26.UAS}

(Lawrence et al., 2000)

Delta^UAS.cHa has wing phenotype, non-suppressible by Scer\GAL4^ptc-559.1/N^{ECNΔ10-12.UAS}

(Lawrence et al., 2000)

Enhancer of

Statement

Reference

Delta^UAS.cHa/Scer\GAL4^{Ubx-lac1-Gal4} is an enhancer of haltere phenotype of Ubx^lac1-GAL4

(Mohit et al., 2006)

Delta^UAS.cHa/Scer\GAL4^{Ubx-lac1-Gal4} is an enhancer of wing hair | ectopic phenotype of Ubx^lac1-GAL4

(Mohit et al., 2006)

Delta^UAS.cHa/Scer\GAL4^{Ubx-lac1-Gal4} is an enhancer of wing margin bristle | ectopic phenotype of Ubx^lac1-GAL4

(Mohit et al., 2006)

Suppressor of

Statement

Reference

Delta^UAS.cHa/Scer\GAL4^h-H10 is a suppressor of photoreceptor cell R8 | somatic clone | third instar larval stage phenotype of Rbf^15aΔ, rno³

(Steele et al., 2009)

Delta^UAS.cHa/Scer\GAL4^dpp.blk1 is a suppressor of wing disc phenotype of Ser^CS94/Ser^RX106

(Klein and Martinez Arias, 1999)

Delta^UAS.cHa/Scer\GAL4^dpp.blk1 is a suppressor of wing phenotype of ap^UGO35

(Klein and Martinez Arias, 1998)

NOT Suppressor of

Statement

Reference

Scer\GAL4^pros.PMG/Delta^UAS.cHa is a non-suppressor of U neuron phenotype of spdo^G104

(O'Connor-Giles and Skeath, 2003)

Scer\GAL4^pros.PMG/Delta^UAS.cHa is a non-suppressor of larval RP2 motor neuron phenotype of spdo^G104

(O'Connor-Giles and Skeath, 2003)

Delta^UAS.cHa/Scer\GAL4^dpp.blk1 is a non-suppressor of wing disc phenotype of Psn^I2/Psn^B3

(Klein et al., 2000)

Delta^UAS.cHa/Scer\GAL4^dpp.blk1 is a non-suppressor of wing margin phenotype of Psn^I2/Psn^B3

(Klein et al., 2000)

Delta^UAS.cHa/Scer\GAL4^dpp.blk1 is a non-suppressor of wing disc phenotype of Su(H)⁸/Su(H)²

(Klein et al., 2000)

Other

Statement

Reference

Delta^UAS.cHa, Scer\GAL4^bi-omb-Gal4, tsh^UAS.cGa has eye | ventral phenotype

(Singh et al., 2004)

Delta^UAS.cHa, Scer\GAL4^bi-omb-Gal4, tsh^UAS.cGa has eye disc | dorsal phenotype

(Singh et al., 2004)

Delta^UAS.cHa, Scer\GAL4^bi-omb-Gal4, tsh^UAS.cGa has eye disc | ventral phenotype

(Singh et al., 2004)

Delta^UAS.cHa, Scer\GAL4^bi-omb-Gal4, tsh^UAS.cGa has eye | dorsal phenotype

(Singh et al., 2004)

Delta^UAS.cHa, Scer\GAL4^Act5C.PI, tsh^UAS.cGa has eye disc | dorsal | somatic clone phenotype

(Singh et al., 2004)

Delta^UAS.cHa, Scer\GAL4^Act5C.PI, tsh^UAS.cGa has eye disc | ventral | somatic clone phenotype

(Singh et al., 2004)

Delta^UAS.cHa, Scer\GAL4^Act5C.PI, tsh^UAS.cGa has eye | dorsal | somatic clone phenotype

(Singh et al., 2004)

Delta^UAS.cHa, Scer\GAL4^Act5C.PI, tsh^UAS.cGa has eye | ventral | somatic clone phenotype

(Singh et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Dl^Scer\UAS.cHa under the control of Scer\GAL4^h-H10 significantly suppresses the multiple photoreceptor-R8 phenotype in rno³; Rbf^15aΔ somatic clones in the eye.

(Steele et al., 2009)

The following heterozygous mutations suppress the large eye phenotype of Scer\GAL4^ey.PH > Dl^Scer\UAS.cHa flies: eyg⁶⁸⁵, Df(1)N-54l9, upd1⁵⁴ and hop²⁵. Heterozygosity for either dome^G0218, dome^G0282 or Stat92E^j6C8 fails to suppress this phenotype.

(Reynolds-Kenneally and Mlodzik, 2005)

Co-expression of tsh^Scer\UAS.cGa and Dl^Scer\UAS.cHa under the control of Scer\GAL4^bi-omb-Gal4 results in eye enlargements on both dorsal and ventral margins in both the eye disc and the adult eye. Ventrally and dorsally located clones in the eye co-expressing tsh^Scer\UAS.cGa and Dl^Scer\UAS.cHa under the control of Scer\GAL4^Act5C.PI result in overgrowth.

(Singh et al., 2004)

The ectopic margin caused by expression of Dl^Scer\UAS.cHa under the control of Scer\GAL4^ptc-559.1 is suppressed by coexpression of N^ECN.Scer\UAS, except for a small patch on the dorsal side, and the wing is almost wild type in shape. The wing phenotype caused by expression of Dl^Scer\UAS.cHa under the control of Scer\GAL4^ptc-559.1 is not suppressed by coexpression of N^{ECNΔ10-12.Scer\UAS} or N^{ECNΔ17-19;24-26.Scer\UAS}.

(Lawrence et al., 2000)

The loss of wing disc tissue seen in Ser^RX106/Ser^CS94 animals is rescued by Dl^Scer\UAS.cHa expressed under the control of Scer\GAL4^dpp.blk1.

(Klein and Martinez Arias, 1999)

The loss of wing seen in ap^UGO35 homozygotes is partially rescued by Dl^Scer\UAS.cHa expressed under the control of Scer\GAL4^dpp.blk1.

(Klein and Martinez Arias, 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

Delta^UAS.cHa/Scer\GAL4^VP16.arm fails to rescue Delta^RevF10

(Seugnet et al., 1997)

Comments

Scer\GAL4^{arm.T:Hsim\VP16} mediated expression fails to rescue Dl^RevF10 embryos.

(Seugnet et al., 1997)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

5614

w¹¹¹⁸; P{UAS-Delta.H}MH1

Kyoto

108332

w¹¹¹⁸; P{UAS-Delta.H}MH1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Delta^UAS.cHa

Dl^Scer\UAS.cHa

Dl^UAS.cHa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Haenlin

(Jonsson and Knust, 1996)

Secondary FlyBase IDs

References (31)

Report Sections

Open Close