FlyBase Allele Report: Dmel\mib1[EY09780]

General Information

Symbol

Dmel\mib1^EY09780

Species

D. melanogaster

Name

FlyBase ID

FBal0318875

Feature type

allele

Associated gene

Dmel\mib1

Associated Insertion(s)

P{EPgy2}mib1^EY09780

Carried in Construct

Also Known As

mib1^EY9780, mib^EY9780, D-mib¹

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Wang and Struhl, 2005)

loss of function allele

(Le Borgne et al., 2005, Pitsouli and Delidakis, 2005)

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{EPgy2}mib1^EY09780

Cytology

Description

The P{EPgy2} insertion is within the first exon of mib1.

(Le Borgne et al., 2005)

P{EPgy2}mib1^EY09780 is inserted 96 nucleotides upstream of the putative mib1 translation start site, although the promoter of the EP transgene points away from the mib1 coding sequence.

(Wang and Struhl, 2005)

Allele components

Component

Use(s)

Inserted element

P{EPgy2}

misexpression element

Regulatory region(s)

UASp

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:16,011,745..16,011,745 [+]3L:16,011,745..16,011,745 [+]

(Kalodimou et al., 2023, Berndt et al., 2017, Cooper and Kennison, 2011, Monastirioti et al., 2010, Lai et al., 2005, Le Borgne et al., 2005, Wang and Struhl, 2005, Gene Disruption Project members, 2001-, FlyBase, 1992-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye

(Lai et al., 2005)

eye | pharate adult stage

(Pitsouli and Delidakis, 2005)

eye disc

(Wang and Struhl, 2005)

eye disc (with Df(3L)brm11)

(Wang and Struhl, 2005)

leg

(Lai et al., 2005, Le Borgne et al., 2005)

leg | pharate adult stage

(Pitsouli and Delidakis, 2005)

macrochaeta | ectopic

(Le Borgne et al., 2005)

macrochaeta | ectopic | somatic clone

(Wang and Struhl, 2005)

macrochaeta | pharate adult stage

(Pitsouli and Delidakis, 2005)

microchaeta | ectopic | somatic clone

(Wang and Struhl, 2005)

microchaeta | pharate adult stage

(Pitsouli and Delidakis, 2005)

scutum & macrochaeta | somatic clone

(Wang and Struhl, 2005)

scutum & microchaeta | somatic clone

(Wang and Struhl, 2005)

wing

(Lai et al., 2005)

wing | pharate adult stage

(Pitsouli and Delidakis, 2005)

wing | P-stage

(Le Borgne et al., 2005)

wing disc

(Lai et al., 2005, Wang and Struhl, 2005)

wing disc (with Df(3L)brm11)

(Wang and Struhl, 2005)

wing disc (with mib1³)

(Le Borgne et al., 2005)

Detailed Description

Statement

Reference

Pharate mib1^EY97600 flies are eyeless, have vestigial wings, and display squat legs lacking joints. mib1^EY97600 mutant wing discs display a severely reduced wing pouch. Sensory organ precursors are singularized normally.

(Lai et al., 2005)

Mutant legs show growth and elongation defects that result in short unsegmented legs.

(Le Borgne et al., 2005)

mib1^EY09780 mutant clones do not exhibit lateral inhibition defects in third instar nota. mib1^EY09780 homozygous mutants survive to the pupal stage and have a good percentage of pharate adult escapers. These have small, almost non-existent, eyes and wings, and short legs. mib1^EY09780 pharate adults exhibit a mild increase in microchaete density and only occasional macrochaete duplications.

(Pitsouli and Delidakis, 2005)

mib1^EY09780 homozygous larvae, as well as mib1^EY09780/Df(3L)brm11 hemizygotes have abnormally small wing and eye imaginal discs and die as pupae. mib1^EY09780 mutant clones form normal patterns of macro- and microchaetes, except that the density of microchaetes is somewhat higher than normal and some macrochaetes (particularly scutellar bristles) are occasionally duplicated. However, each bristle organ itself appears morphologically normal.

(Wang and Struhl, 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

mib1³/mib1^EY09780 has wing disc phenotype, suppressible by N^{FLN.ΔCdc10.UAS}/Scer\GAL4^Ser.PGF

(Le Borgne et al., 2005)

mib1³/mib1^EY09780 has wing disc phenotype, suppressible by Scer\GAL4^Ser.PGF/neur^UAS.cPa

(Le Borgne et al., 2005)

NOT suppressed by

Statement

Reference

mib1³/mib1^EY09780 has wing disc phenotype, non-suppressible by Scer\GAL4^Ser.PGF/Ser^UAS.cSa

(Le Borgne et al., 2005)

Enhancer of

Statement

Reference

mib1^EY09780 is an enhancer of sensory mother cell | third instar larval stage | ectopic | somatic clone phenotype of Delta^RevF10

(Pitsouli and Delidakis, 2005)

mib1^EY09780 is an enhancer of sensory mother cell | ectopic | somatic clone phenotype of neur¹

(Pitsouli and Delidakis, 2005)

mib1^EY09780 is an enhancer of sensory mother cell | ectopic phenotype of neur¹

(Pitsouli and Delidakis, 2005)

Additional Comments

Genetic Interactions

Statement

Reference

mib1^EY09780 Ser^RX106 double mutant clones do not exhibit lateral inhibition defects in third instar nota. mib1^EY09780 neur¹ third instar nota clones exhibit at least eight sensory organ precursors (SOPs) in approximately 82.5% of SOP positions scored. Approximately 17.5% of SOP positions display between four and eight ectopic SOPs. No wild-type SOPs are found. mib1^EY09780 Dl^RevF10 third instar nota clones exhibit at least eight sensory organ precursors (SOPs) in approximately 40% of SOP positions scored. Approximately 45% of SOP positions display between four and eight ectopic SOPs, while approximately 5% exhibit between one and four SOPs, with 10% exhibiting one SOP, as in wild-type. Induction of neur¹ mutant clones in a homozygous mib1^EY09780 background results in a large number of ectopic sensory organ precursors.

(Pitsouli and Delidakis, 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

mib1⁴

(Le Borgne et al., 2005)

mib1³

(Le Borgne et al., 2005)

Rescued by

Df(3L)mib1²/mib1^EY09780 is rescued by mib1^UAS.cLa

(Le Borgne et al., 2005)

mib1^EY09780 is rescued by Scer\GAL4^nub-AC-62/mib1^UAS.cWa

(Wang and Struhl, 2005)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

17603

y¹ w^67c23; P{EPgy2}mib1^EY09780/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

CG5841^EY09780

D-mib¹

(Lai et al., 2005, Le Borgne et al., 2005)

mib1^EY09780

(Berndt et al., 2017, McMillan et al., 2015)

mib1^EY09870

(Troost et al., 2023)

mib1^EY97600

mib1^EY9780

(Monastirioti et al., 2010, Pitsouli and Delidakis, 2005)

mib^EY9780

(Kalodimou et al., 2023, Daskalaki et al., 2011)

mind-bomb^EY09780

Name Synonyms

Secondary FlyBase IDs

FBal0161232
FBal0178894

References (14)

Report Sections

Open Close