FlyBase Allele Report: Dmel\neur[UAS.cPa]

General Information

Symbol

Dmel\neur^UAS.cPa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Pavlopoulos

FlyBase ID

FBal0141022

Feature type

allele

Associated gene

Dmel\neur

Associated Insertion(s)

Carried in Construct

P{UAS-neur.P}

Also Known As

UAS-neur

Key Links

Transgenic product class

cDNA

(Pavlopoulos et al., 2001)

wild_type

(Pavlopoulos et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Pavlopoulos et al., 2001)

wild_type

(Pavlopoulos et al., 2001)

Mutagen

in vitro construct

(Klueg et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-neur.P}

Cytology

Description

Expression of nucleotides 247-2637 of the neur cDNA (includes 30 bp of 5'UTR and 95 bp of 3'UTR) is under the control of UASt sequences.

(Pavlopoulos et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

neur

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory, with Scer\GAL4^c747, Scer\GAL80^ts.αTub84B

(Pavlopoulos et al., 2008)

abnormal memory, with Scer\GAL4^Mef2.247

(Pavlopoulos et al., 2008)

abnormal memory | temperature conditional, with Scer\GAL4^c747

(Pavlopoulos et al., 2008)

Phenotype Manifest In

Detailed Description

Statement

Reference

Overexpression of neur^Scer\UAS.cPa in the mushroom bodies (under the control of Scer\GAL4^Mef2.247 or Scer\GAL4^c747) does not affect the response to conditioned or unconditioned stimuli, nor the gross morphology of the mushroom bodies. Ninety-minute memory is the same in overexpression and wild-type flies. In contrast, memory assessed 260 minutes later is significantly higher in neur^Scer\UAS.cPa-expressing flies. In addition, elevation of neur in the mushroom bodies results in higher 24-hour memory assessed after conditioning with a single cycle of 12 conditioned-stimuli/unconditioned stimuli pairings.

Expression of neur^Scer\UAS.cPa in the γ-lobes under the control of Scer\GAL4^H24 does not affect memory formation.

Neither 260-minute nor 24-hour memory is affected in flies expressing neur^Scer\UAS.cPa in agr;/β-lobe neurons.

Restricting expression of neur^Scer\UAS.cPa to the adult α and β mushroom body lobes, through Scer\GAL4^c747 and Scer\GAL80^ts.αTub84B does not affect the ability of neur^Scer\UAS.cPa overexpression to enhance 24 hour memory.

Flies overexpressing neur^Scer\UAS.cPa under the control of Scer\GAL4^c747 display enhanced 24-hour memory when kept at 29[o]C either for the entire period or specifically for the first 3 hours after training. Cold-shock, by training the animals with a single conditioned stimuli/unconditioned stimuli cycle, and then a 10 minute cold shock 2 hours after training, collapses the enhanced 260-minute memory of neur^Scer\UAS.cPa-overexpressing flies. The enhanced 24 hour memory in neur^Scer\UAS.cPa-overexpressing flies is eliminated by the protein synthesis inhibitor cycloheximide.

(Pavlopoulos et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Scer\GAL4^pros.PMG, Delta^UAS.cLa, neur^UAS.cPa is a non-suppressor of abnormal neuroanatomy phenotype of spdo^G104

(Babaoglan et al., 2009)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^twi.PG, Delta^UAS.cLa, neur^UAS.cPa is a suppressor of embryonic pericardial cell phenotype of spdo^G104

(Babaoglan et al., 2009)

Scer\GAL4^Ser.PGF/neur^UAS.cPa is a suppressor of wing disc phenotype of mib1³/mib1^EY09780

(Le Borgne et al., 2005)

NOT Suppressor of

Statement

Reference

Scer\GAL4^pros.PMG, Delta^UAS.cLa, neur^UAS.cPa is a non-suppressor of dMP2 neuron | ectopic phenotype of spdo^G104

(Babaoglan et al., 2009)

Scer\GAL4^pros.PMG, Delta^UAS.cLa, neur^UAS.cPa is a non-suppressor of vMP2 neuron phenotype of spdo^G104

(Babaoglan et al., 2009)

Scer\GAL4^pros.PMG, Delta^UAS.cLa, neur^UAS.cPa is a non-suppressor of embryonic pericardial cell phenotype of spdo^G104

(Babaoglan et al., 2009)

Scer\GAL4^{kirre-rP298-G4}/neur^UAS.cPa is a non-suppressor of embryonic/larval somatic muscle cell | embryonic stage 16 phenotype of mib2¹

(Nguyen et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of neur^Scer\UAS.cPa and Dl^Scer\UAS.cLa under the control of Scer\GAL4^twi.PG has no effect on eve-positive pericardial cell numbers.

Expression of neur^Scer\UAS.cPa and Dl^Scer\UAS.cLa under the control of Scer\GAL4^twi.PG in a spdo^G104 mutant background does not alter the number of eve-positive pericardial cells compared to spdo^G104 mutant embryos.

Co-expression of neur^Scer\UAS.cPa and Dl^Scer\UAS.cLa under the control of Scer\GAL4^twi.PG in a spdo^G104 mutant background partially restore svp-positive pericardial cell development by promoting more asymmetric divisions of progenitor cells.

(Babaoglan et al., 2009)

The muscle defects seen in stage 16 homozygous mib2¹ embryos are not rescued by expression of neur^Scer\UAS.cPa under the control of Scer\GAL4^kirre-rP298.

(Nguyen et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^c747/neur^UAS.cPa rescues neur¹

(Pavlopoulos et al., 2008)

Comments

Expression of neur^Scer\UAS.cPa under the control of Scer\GAL4^c747 fully rescues 260-minute memory after conditioned and unconditioned stimuli pairings in neur¹ mutants.

(Pavlopoulos et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

26688

y¹ w^67c23; P{UAS-neur.P}2

Notes on Origin

Discoverer

Comments

Carried in plasmid " pUAST:NeurWT ", transfected into S2 cells and used in cell-cell aggregation assays.

(Klueg et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

neur^Scer\UAS.cPa

neur^UAS.cPa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Pavlopoulos

(Klueg et al., 2002)

Secondary FlyBase IDs

References (8)

Report Sections

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