Caf1^short/Caf1^med flies expressing Caf1^Scer\UAS.cAa under the control of Scer\GAL4^Ubi and raised at 18[o]C during the third larval instar stage have disorganised kidney-shaped eyes with abnormalities in the arrangement of rhabdomeres (including loss of small central rhabdomeres) and show homeotic transformation of the arista to leg-like structures that occasionally end in claws. These defects are not seen if the animals are raised at 25[o]C during the third larval instar stage.

The eyes of flies expressing Caf1^Scer\UAS.cAa under the control of Scer\GAL4^ey.PU and raised at 25[o]C are disorganised, containing reduced numbers of interommatidial bristles and being slightly smaller than those of controls. The phenotype is more severe at 30[o]C or with increased copy number of the Caf1^Scer\UAS.cAa and Scer\GAL4^ey.PU constructs.

Third larval instar eye discs expressing Caf1^Scer\UAS.cAa under the control of Scer\GAL4^ey.PU show significant apoptosis in the anterior region of the disc.