FlyBase Allele Report: Dmel\sqh[T20A.S21A.UAS]

General Information

Symbol

Dmel\sqh^{T20A.S21A.UAS}

Species

D. melanogaster

Name

FlyBase ID

FBal0266156

Feature type

allele

Associated gene

Dmel\sqh

Associated Insertion(s)

Carried in Construct

P{UAS-sqh.AA}

Key Links

Transgenic product class

missense_variant

(Dorsten et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Dorsten et al., 2007)

Mutagen

in vitro construct

(Dorsten et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-sqh.AA}

Cytology

Description

UASt regulatory sequences drive expression of a mutant sqh carrying amino acid substitutions T20A and S21A.

(Dorsten et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

sqh

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

short lived | calorie restriction conditional, with Scer\GAL4^Cg.PA

(Tang et al., 2011)

Phenotype Manifest In

Detailed Description

Statement

Reference

Compared with wild-type flies, those expressing sqh^{T20A.S21A.Scer\UAS} under the control of Scer\GAL4^Cg.PA show a significantly shortened lifespan under starvation conditions.

(Tang et al., 2011)

Stage 16 embryos overexpressing sqh^{T20A.S21A.Scer\UAS} driven by Scer\GAL4^ftz.ng rarely display axon bundles that cross the midline inappropriately.

(Dorsten et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, sqh^{T20A.S21A.UAS} has visible phenotype, suppressible by Scer\GAL4^ptc-559.1, sqh^{T20A.S21A.UAS}

(Tang et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, sqh^{T20A.S21A.UAS} has visible phenotype, non-suppressible by Atg1^RNAi.UAS, Scer\GAL4^ptc-559.1

(Tang et al., 2011)

Suppressor of

Statement

Reference

Scer\GAL4^ptc-559.1, sqh^{T20A.S21A.UAS} is a suppressor of visible phenotype of Scer\GAL4^ptc-559.1, sqh^{T20A.S21A.UAS}

(Tang et al., 2011)

sqh^{T20A.S21A.UAS}, Scer\GAL4^ftz.ng is a suppressor of abnormal neuroanatomy | embryonic stage 16 phenotype of Rho1^V14.UAS, Scer\GAL4^ftz.ng, fra^UAS.cKa

(Dorsten et al., 2007)

sqh^{T20A.S21A.UAS}, Scer\GAL4^ftz.ng is a suppressor of abnormal neuroanatomy | embryonic stage 16 phenotype of Ggal\MLCK^ct.UAS, Rho1^V14.UAS, Scer\GAL4^ftz.ng

(Dorsten et al., 2007)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, sqh^{T20A.S21A.UAS} has anterior crossvein phenotype, suppressible by Scer\GAL4^ptc-559.1, sqh^{T20A.S21A.UAS}

(Tang et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, sqh^{T20A.S21A.UAS} has anterior crossvein phenotype, non-suppressible by Atg1^RNAi.UAS, Scer\GAL4^ptc-559.1

(Tang et al., 2011)

Suppressor of

Statement

Reference

Scer\GAL4^ptc-559.1, sqh^{T20A.S21A.UAS} is a suppressor of anterior crossvein phenotype of Scer\GAL4^ptc-559.1, sqh^{T20A.S21A.UAS}

(Tang et al., 2011)

sqh^{T20A.S21A.UAS}, Scer\GAL4^Act5C.PP is a suppressor of fat body | somatic clone | calorie restriction conditional phenotype of Atg12^RNAi.UAS, Scer\GAL4^Act5C.PP

(Tang et al., 2011)

sqh^{T20A.S21A.UAS}, Scer\GAL4^Act5C.PP is a suppressor of autophagosome | somatic clone | calorie restriction conditional phenotype of Atg12^RNAi.UAS, Scer\GAL4^Act5C.PP

(Tang et al., 2011)

sqh^{T20A.S21A.UAS}, Scer\GAL4^ftz.ng is a suppressor of commissure | embryonic stage 16 phenotype of Rho1^V14.UAS, Scer\GAL4^ftz.ng, fra^UAS.cKa

(Dorsten et al., 2007)

sqh^{T20A.S21A.UAS}, Scer\GAL4^ftz.ng is a suppressor of larval ventral nerve cord commissure | embryonic stage 16 phenotype of Rho1^V14.UAS, Scer\GAL4^ftz.ng, fra^UAS.cKa

(Dorsten et al., 2007)

sqh^{T20A.S21A.UAS}, Scer\GAL4^ftz.ng is a suppressor of commissure | embryonic stage 16 phenotype of Ggal\MLCK^ct.UAS, Rho1^V14.UAS, Scer\GAL4^ftz.ng

(Dorsten et al., 2007)

sqh^{T20A.S21A.UAS}, Scer\GAL4^ftz.ng is a suppressor of larval ventral nerve cord commissure | embryonic stage 16 phenotype of Ggal\MLCK^ct.UAS, Rho1^V14.UAS, Scer\GAL4^ftz.ng

(Dorsten et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The sqa^Scer\UAS-induced anterior crossvein defects can be suppressed by co-expressing sqh^{T20A.S21A.Scer\UAS} under the control of Scer\GAL4^ptc-559.1.

The sqa^Scer\UAS-induced anterior crossvein defects are not suppressed by co-expressing Atg1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ptc-559.1.

Co-expression of sqh^{T20A.S21A.Scer\UAS} with Atg12^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PP strongly suppresses the Atg12^{dsRNA.Scer\UAS}-induced fat body phenotype.

(Tang et al., 2011)

Co-expression of sqh^{T20A.S21A.Scer\UAS} under the control of Scer\GAL4^ftz.ng suppresses the midline crossing phenotype in embryos expressing both Rho1^V14.Scer\UAS and fra^Scer\UAS.cKa.

Co-expression of sqh^{T20A.S21A.Scer\UAS} under the control of Scer\GAL4^ftz.ng suppresses the midline crossing phenotype in embryos expressing both Rho1^V14.Scer\UAS and Ggal\MLCK^ct.Scer\UAS.

(Dorsten et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

sqh^{T20A.S21A.Scer\UAS}

sqh^{T20A.S21A.UAS}

Name Synonyms

Secondary FlyBase IDs

References (2)

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